| Sumario: | Wheat is one of the most important crops for global food security and represents a main source of food and
income for millions of smallholder farmers worldwide. Pseudomonas syringae pv. syringae (Pss) is the causal
agent of bacterial leaf blight or leaf necrosis disease with specific symptoms on wheat leaves and spikes and
in good conditions it can reach to the seeds to be seed borne disease. Yield losses caused by Pss can reach
up to 50% or more depending on the crop, time of infection, and region. Therefore, developing and
implementing effective management strategies for bacterial diseases is very important to reduce yield and
quality loss. However, accurate detection of diseases is the first essential step for effective management
strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the
occurrence and development of crop diseases. This report describes a validated conventional polymerase
chain reaction (PCR) assay for Pss detection and characterization applied by ICARDA’s GHU.
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