Cryopreservation of Abies alba embryogenic tissues by slow-freezing method

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liqui...

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Bibliographic Details
Main Authors: Salaj, Terezia, Panis, Bartholomeus, Klubicová, Katarina, Salaj, Jan
Format: Journal Article
Language:Inglés
Published: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca 2022
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Online Access:https://hdl.handle.net/10568/126396

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