Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liqui...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/126396 |
| _version_ | 1855538635225432064 |
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| author | Salaj, Terezia Panis, Bartholomeus Klubicová, Katarina Salaj, Jan |
| author_browse | Klubicová, Katarina Panis, Bartholomeus Salaj, Jan Salaj, Terezia |
| author_facet | Salaj, Terezia Panis, Bartholomeus Klubicová, Katarina Salaj, Jan |
| author_sort | Salaj, Terezia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently,
the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines
recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the
accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of
germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic
cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed. |
| format | Journal Article |
| id | CGSpace126396 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca |
| publisherStr | University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca |
| record_format | dspace |
| spelling | CGSpace1263962025-11-11T19:05:14Z Cryopreservation of Abies alba embryogenic tissues by slow-freezing method Salaj, Terezia Panis, Bartholomeus Klubicová, Katarina Salaj, Jan pinophyta cryopreservation regeneration embryonic development criopreservación regeneración desarrollo embrionario Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed. 2022-12-06 2022-12-29T10:41:49Z 2022-12-29T10:41:49Z Journal Article https://hdl.handle.net/10568/126396 en Open Access application/pdf University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Salaj, T.; Panis, B.; Klubicova, K.; Salaj, J. (2022) Cryopreservation of Abies alba embryogenic tissues by slow-freezing method. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50(4) : 12770 15 p. ISSN: 0255-965X |
| spellingShingle | pinophyta cryopreservation regeneration embryonic development criopreservación regeneración desarrollo embrionario Salaj, Terezia Panis, Bartholomeus Klubicová, Katarina Salaj, Jan Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title | Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title_full | Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title_fullStr | Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title_full_unstemmed | Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title_short | Cryopreservation of Abies alba embryogenic tissues by slow-freezing method |
| title_sort | cryopreservation of abies alba embryogenic tissues by slow freezing method |
| topic | pinophyta cryopreservation regeneration embryonic development criopreservación regeneración desarrollo embrionario |
| url | https://hdl.handle.net/10568/126396 |
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