Cryopreservation of Abies alba embryogenic tissues by slow-freezing method

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liqui...

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Main Authors: Salaj, Terezia, Panis, Bartholomeus, Klubicová, Katarina, Salaj, Jan
Format: Journal Article
Language:Inglés
Published: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca 2022
Subjects:
Online Access:https://hdl.handle.net/10568/126396
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author Salaj, Terezia
Panis, Bartholomeus
Klubicová, Katarina
Salaj, Jan
author_browse Klubicová, Katarina
Panis, Bartholomeus
Salaj, Jan
Salaj, Terezia
author_facet Salaj, Terezia
Panis, Bartholomeus
Klubicová, Katarina
Salaj, Jan
author_sort Salaj, Terezia
collection Repository of Agricultural Research Outputs (CGSpace)
description Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed.
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language Inglés
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spelling CGSpace1263962025-11-11T19:05:14Z Cryopreservation of Abies alba embryogenic tissues by slow-freezing method Salaj, Terezia Panis, Bartholomeus Klubicová, Katarina Salaj, Jan pinophyta cryopreservation regeneration embryonic development criopreservación regeneración desarrollo embrionario Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed. 2022-12-06 2022-12-29T10:41:49Z 2022-12-29T10:41:49Z Journal Article https://hdl.handle.net/10568/126396 en Open Access application/pdf University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Salaj, T.; Panis, B.; Klubicova, K.; Salaj, J. (2022) Cryopreservation of Abies alba embryogenic tissues by slow-freezing method. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50(4) : 12770 15 p. ISSN: 0255-965X
spellingShingle pinophyta
cryopreservation
regeneration
embryonic development
criopreservación
regeneración
desarrollo embrionario
Salaj, Terezia
Panis, Bartholomeus
Klubicová, Katarina
Salaj, Jan
Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title_full Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title_fullStr Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title_full_unstemmed Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title_short Cryopreservation of Abies alba embryogenic tissues by slow-freezing method
title_sort cryopreservation of abies alba embryogenic tissues by slow freezing method
topic pinophyta
cryopreservation
regeneration
embryonic development
criopreservación
regeneración
desarrollo embrionario
url https://hdl.handle.net/10568/126396
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AT panisbartholomeus cryopreservationofabiesalbaembryogenictissuesbyslowfreezingmethod
AT klubicovakatarina cryopreservationofabiesalbaembryogenictissuesbyslowfreezingmethod
AT salajjan cryopreservationofabiesalbaembryogenictissuesbyslowfreezingmethod