Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/108663 |
| _version_ | 1855519558090096640 |
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| author | Nkere, C.K. Oyekanmi, J.O. Silva, G. Bömer, M. Atiri, G.I. Onyeka, J. Maroya, N.G. Seal, S.E. Kumar, P. Lava |
| author_browse | Atiri, G.I. Bömer, M. Kumar, P. Lava Maroya, N.G. Nkere, C.K. Onyeka, J. Oyekanmi, J.O. Seal, S.E. Silva, G. |
| author_facet | Nkere, C.K. Oyekanmi, J.O. Silva, G. Bömer, M. Atiri, G.I. Onyeka, J. Maroya, N.G. Seal, S.E. Kumar, P. Lava |
| author_sort | Nkere, C.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity. |
| format | Journal Article |
| id | CGSpace108663 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1086632025-11-11T10:09:05Z Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) Nkere, C.K. Oyekanmi, J.O. Silva, G. Bömer, M. Atiri, G.I. Onyeka, J. Maroya, N.G. Seal, S.E. Kumar, P. Lava yams viruses plant viruses plant condition plant diseases production virology A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity. 2018-04 2020-07-03T08:48:56Z 2020-07-03T08:48:56Z Journal Article https://hdl.handle.net/10568/108663 en Open Access application/pdf Springer Nkere, C.K., Oyekanmi, J.O., Silva, G., Bömer, M., Atiri, G.I., Onyeka, J., ... & Kumar, P.L. (2018). Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP). Archives of Virology, 163(4), 1057-1061. |
| spellingShingle | yams viruses plant viruses plant condition plant diseases production virology Nkere, C.K. Oyekanmi, J.O. Silva, G. Bömer, M. Atiri, G.I. Onyeka, J. Maroya, N.G. Seal, S.E. Kumar, P. Lava Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title | Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title_full | Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title_fullStr | Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title_full_unstemmed | Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title_short | Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) |
| title_sort | chromogenic detection of yam mosaic virus by closed tube reverse transcription loop mediated isothermal amplification ct rt lamp |
| topic | yams viruses plant viruses plant condition plant diseases production virology |
| url | https://hdl.handle.net/10568/108663 |
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