Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2

COVID-19 is the most rapidly growing pandemic in modern time, and the need for serological testing is most urgent. Although the diagnostics of acute patients by RT-PCR is both efficient and specific, we are also crucially in need of serological tools for investigating antibody responses and assessin...

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Autores principales: Hoffman, T., Nissen, K., Krambrich, J., Rönnberg, B., Akaberi, D., Esmaeilzadeh, M., Salaneck, E., Lindahl, Johanna F., Lundkvist, Å.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Informa UK Limited 2020
Materias:
Acceso en línea:https://hdl.handle.net/10568/107986
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author Hoffman, T.
Nissen, K.
Krambrich, J.
Rönnberg, B.
Akaberi, D.
Esmaeilzadeh, M.
Salaneck, E.
Lindahl, Johanna F.
Lundkvist, Å.
author_browse Akaberi, D.
Esmaeilzadeh, M.
Hoffman, T.
Krambrich, J.
Lindahl, Johanna F.
Lundkvist, Å.
Nissen, K.
Rönnberg, B.
Salaneck, E.
author_facet Hoffman, T.
Nissen, K.
Krambrich, J.
Rönnberg, B.
Akaberi, D.
Esmaeilzadeh, M.
Salaneck, E.
Lindahl, Johanna F.
Lundkvist, Å.
author_sort Hoffman, T.
collection Repository of Agricultural Research Outputs (CGSpace)
description COVID-19 is the most rapidly growing pandemic in modern time, and the need for serological testing is most urgent. Although the diagnostics of acute patients by RT-PCR is both efficient and specific, we are also crucially in need of serological tools for investigating antibody responses and assessing individual and potential herd immunity. We evaluated a commercially available test developed for rapid (within 15 minutes) detection of SARS-CoV-2-specific IgM and IgG by 29 PCR-confirmed COVID-19 cases and 124 negative controls. The results revealed a sensitivity of 69% and 93.1% for IgM and IgG, respectively, based solely on PCR-positivity due to the absence of a serological gold standard. The assay specificities were shown to be 100% for IgM and 99.2% for IgG. This indicates that the test is suitable for assessing previous virus exposure, although negative results may be unreliable during the first weeks after infection. More detailed studies on antibody responses during and post infection are urgently needed.
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spelling CGSpace1079862023-03-17T17:46:25Z Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2 Hoffman, T. Nissen, K. Krambrich, J. Rönnberg, B. Akaberi, D. Esmaeilzadeh, M. Salaneck, E. Lindahl, Johanna F. Lundkvist, Å. diagnosis zoonoses covid-19 COVID-19 is the most rapidly growing pandemic in modern time, and the need for serological testing is most urgent. Although the diagnostics of acute patients by RT-PCR is both efficient and specific, we are also crucially in need of serological tools for investigating antibody responses and assessing individual and potential herd immunity. We evaluated a commercially available test developed for rapid (within 15 minutes) detection of SARS-CoV-2-specific IgM and IgG by 29 PCR-confirmed COVID-19 cases and 124 negative controls. The results revealed a sensitivity of 69% and 93.1% for IgM and IgG, respectively, based solely on PCR-positivity due to the absence of a serological gold standard. The assay specificities were shown to be 100% for IgM and 99.2% for IgG. This indicates that the test is suitable for assessing previous virus exposure, although negative results may be unreliable during the first weeks after infection. More detailed studies on antibody responses during and post infection are urgently needed. 2020-01-01 2020-04-14T18:22:08Z 2020-04-14T18:22:08Z Journal Article https://hdl.handle.net/10568/107986 en Open Access Informa UK Limited Hoffman, T., Nissen, K., Krambrich, J., Rönnberg, B., Akaberi, D., Esmaeilzadeh, M., Salaneck, E., Lindahl, J. and Lundkvist, Å. 2020. Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2. Infection Ecology & Epidemiology 10(1): 1754538.
spellingShingle diagnosis
zoonoses
covid-19
Hoffman, T.
Nissen, K.
Krambrich, J.
Rönnberg, B.
Akaberi, D.
Esmaeilzadeh, M.
Salaneck, E.
Lindahl, Johanna F.
Lundkvist, Å.
Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title_full Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title_fullStr Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title_full_unstemmed Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title_short Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2
title_sort evaluation of a covid 19 igm and igg rapid test an efficient tool for assessment of past exposure to sars cov 2
topic diagnosis
zoonoses
covid-19
url https://hdl.handle.net/10568/107986
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