Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells
Salinity tolerance is associated with Na ‘exclusion’ from, or ‘tissue tolerance’ in, leaves. We investigated whether two contrasting chickpea genotypes, salt-tolerant Genesis836 and salt-sensitive Rupali, differ in leaf tissue tolerance to NaCl. We used X-ray microanalysis to evaluate cellular Na, C...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/105719 |
| _version_ | 1855518333019881472 |
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| author | Kotula, Lukasz Clode, Peta L. Jiménez Serna, Juan de la Cruz Colmer, Timothy D. |
| author_browse | Clode, Peta L. Colmer, Timothy D. Jiménez Serna, Juan de la Cruz Kotula, Lukasz |
| author_facet | Kotula, Lukasz Clode, Peta L. Jiménez Serna, Juan de la Cruz Colmer, Timothy D. |
| author_sort | Kotula, Lukasz |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Salinity tolerance is associated with Na ‘exclusion’ from, or ‘tissue tolerance’ in, leaves. We investigated whether two contrasting chickpea genotypes, salt-tolerant Genesis836 and salt-sensitive Rupali, differ in leaf tissue tolerance to NaCl. We used X-ray microanalysis to evaluate cellular Na, Cl, and K concentrations in various cell types within leaflets and also in secretory trichomes of the two chickpea genotypes in relation to photosynthesis in control and saline conditions. TEM was used to assess the effects of salinity on the ultrastructure of chloroplasts. Genesis836 maintained net photosynthetic rates (A) for the 21 d of salinity treatment (60 mM NaCl), whereas A in Rupali substantially decreased after 11 d. Leaflet tissue [Na] was low in Genesis836 but had increased markedly in Rupali. In Genesis836, Na was accumulated in epidermal cells but was low in mesophyll cells, whereas in Rupali cellular [Na] was high in both cell types. The excessive accumulation of Na in mesophyll cells of Rupali corresponded to structural damage to the chloroplasts. Maintenance of photosynthesis and thus salinity tolerance in Genesis836 was associated with an ability to ‘exclude’ Na from leaflets and in particular from the photosynthetically active mesophyll cells, and to compartmentalize Na in epidermal cells. |
| format | Journal Article |
| id | CGSpace105719 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1057192025-03-13T09:44:02Z Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells Kotula, Lukasz Clode, Peta L. Jiménez Serna, Juan de la Cruz Colmer, Timothy D. cicer arietinum salinity sodium chloroplasts cloroplasto physiology Salinity tolerance is associated with Na ‘exclusion’ from, or ‘tissue tolerance’ in, leaves. We investigated whether two contrasting chickpea genotypes, salt-tolerant Genesis836 and salt-sensitive Rupali, differ in leaf tissue tolerance to NaCl. We used X-ray microanalysis to evaluate cellular Na, Cl, and K concentrations in various cell types within leaflets and also in secretory trichomes of the two chickpea genotypes in relation to photosynthesis in control and saline conditions. TEM was used to assess the effects of salinity on the ultrastructure of chloroplasts. Genesis836 maintained net photosynthetic rates (A) for the 21 d of salinity treatment (60 mM NaCl), whereas A in Rupali substantially decreased after 11 d. Leaflet tissue [Na] was low in Genesis836 but had increased markedly in Rupali. In Genesis836, Na was accumulated in epidermal cells but was low in mesophyll cells, whereas in Rupali cellular [Na] was high in both cell types. The excessive accumulation of Na in mesophyll cells of Rupali corresponded to structural damage to the chloroplasts. Maintenance of photosynthesis and thus salinity tolerance in Genesis836 was associated with an ability to ‘exclude’ Na from leaflets and in particular from the photosynthetically active mesophyll cells, and to compartmentalize Na in epidermal cells. 2019-09-24 2019-11-13T15:35:09Z 2019-11-13T15:35:09Z Journal Article https://hdl.handle.net/10568/105719 en Open Access Oxford University Press Kotula, Lukasz; Clode, Peta L.; Jimenez, Juan De La Cruz & Colmer, Timothy D. (2019). Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells. Journal of Experimental Botany, 70 (18): 4991-5002 |
| spellingShingle | cicer arietinum salinity sodium chloroplasts cloroplasto physiology Kotula, Lukasz Clode, Peta L. Jiménez Serna, Juan de la Cruz Colmer, Timothy D. Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title | Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title_full | Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title_fullStr | Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title_full_unstemmed | Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title_short | Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells |
| title_sort | salinity tolerance in chickpea is associated with the ability to exclude na from leaf mesophyll cells |
| topic | cicer arietinum salinity sodium chloroplasts cloroplasto physiology |
| url | https://hdl.handle.net/10568/105719 |
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