Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids
A developed Near Infrared Reflectance Spectroscopy (NIRS) calibration equation was used for determining provitamin A carotenoids contents of different trials of fresh yellow root cassava genotypes using a total of 50 cassava genotypes scanned twice by NIRS from 400 nm to 2498 nm. The NIRS calibratio...
| Main Authors: | , , , , |
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| Format: | Book Chapter |
| Language: | Inglés |
| Published: |
IM Publications Open LLP
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/101680 |
| _version_ | 1855524621386776576 |
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| author | Alamu, Emmanuel Oladeji Maziya-Dixon, B.B. Felde, T. zum Kulakow, Peter A. Parkes, Elizabeth Y. |
| author_browse | Alamu, Emmanuel Oladeji Felde, T. zum Kulakow, Peter A. Maziya-Dixon, B.B. Parkes, Elizabeth Y. |
| author_facet | Alamu, Emmanuel Oladeji Maziya-Dixon, B.B. Felde, T. zum Kulakow, Peter A. Parkes, Elizabeth Y. |
| author_sort | Alamu, Emmanuel Oladeji |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A developed Near Infrared Reflectance Spectroscopy (NIRS) calibration equation was used for determining provitamin A carotenoids contents of different trials of fresh yellow root cassava genotypes using a total of 50 cassava genotypes scanned twice by NIRS from 400 nm to 2498 nm. The NIRS calibration equations were used to predict the β-cryptoxanthin, 13-cis β-carotene, trans β-carotene, 9-cis β -carotene, total β-carotene and total carotenoid concentrations of the samples. The predicted values for total carotenoids (TC-pred) ranged from 3.93 μg g–1 to 10.51 μg g–1 with mean of 7.07 ± 2.55 μg g–1 for International Collaborative Trials (ICT), 7.97–11.03 μg g–1 fresh weight with mean of 9.40 ± 0.76 μg g–1 for yellow root trial 8 (Multi-location Uniform Yield Trial) and 6.38–10.44 μg g–1 with mean of 8.74 ± 1.07 μg g–1 for yellow root trial 9 (Multilocation Advanced Yield Trial). Total carotenoids results using reference spectrophotometric method (TC-spec) ranged from 2.57 μg g–1 to 9.97 μg g–1 with mean of 5.66 ± 2.99 μg g–1 for ICT, 6.55–8.74 μg g–1 with mean of 7.74 ± 0.64 μg g–1 for yellow root trial 8 and 4.22–11.00 μg g–1 with mean of 7.57 ± 1.54 μg g–1 for yellow root trail 9. There is significant (P ≤ 0.001) positive correlation (r = 0.55) between TC-pred by NIRS and TC-spec. Also, significant (P ≤ 0.001) positive correlation (r = 0.52) exist between trans β-carotene predicted by NIRS and high-performance liquid chromatography reference. The developed NIRS calibration equations could be used to predict total carotenoids and trans β-carotene content of yellow root cassava and serve as rapid and cost-effective screening method for large cassava sample sets. |
| format | Book Chapter |
| id | CGSpace101680 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | IM Publications Open LLP |
| publisherStr | IM Publications Open LLP |
| record_format | dspace |
| spelling | CGSpace1016802025-03-11T12:14:31Z Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids Alamu, Emmanuel Oladeji Maziya-Dixon, B.B. Felde, T. zum Kulakow, Peter A. Parkes, Elizabeth Y. cassava carotenoids carotenes provitamins genotypes near infrared spectroscopy A developed Near Infrared Reflectance Spectroscopy (NIRS) calibration equation was used for determining provitamin A carotenoids contents of different trials of fresh yellow root cassava genotypes using a total of 50 cassava genotypes scanned twice by NIRS from 400 nm to 2498 nm. The NIRS calibration equations were used to predict the β-cryptoxanthin, 13-cis β-carotene, trans β-carotene, 9-cis β -carotene, total β-carotene and total carotenoid concentrations of the samples. The predicted values for total carotenoids (TC-pred) ranged from 3.93 μg g–1 to 10.51 μg g–1 with mean of 7.07 ± 2.55 μg g–1 for International Collaborative Trials (ICT), 7.97–11.03 μg g–1 fresh weight with mean of 9.40 ± 0.76 μg g–1 for yellow root trial 8 (Multi-location Uniform Yield Trial) and 6.38–10.44 μg g–1 with mean of 8.74 ± 1.07 μg g–1 for yellow root trial 9 (Multilocation Advanced Yield Trial). Total carotenoids results using reference spectrophotometric method (TC-spec) ranged from 2.57 μg g–1 to 9.97 μg g–1 with mean of 5.66 ± 2.99 μg g–1 for ICT, 6.55–8.74 μg g–1 with mean of 7.74 ± 0.64 μg g–1 for yellow root trial 8 and 4.22–11.00 μg g–1 with mean of 7.57 ± 1.54 μg g–1 for yellow root trail 9. There is significant (P ≤ 0.001) positive correlation (r = 0.55) between TC-pred by NIRS and TC-spec. Also, significant (P ≤ 0.001) positive correlation (r = 0.52) exist between trans β-carotene predicted by NIRS and high-performance liquid chromatography reference. The developed NIRS calibration equations could be used to predict total carotenoids and trans β-carotene content of yellow root cassava and serve as rapid and cost-effective screening method for large cassava sample sets. 2019-02-19 2019-06-25T11:00:21Z 2019-06-25T11:00:21Z Book Chapter https://hdl.handle.net/10568/101680 en Open Access application/pdf IM Publications Open LLP Alamu, E.O., Maziya-Dixon, B., Zum Felde, T., Kulakow, P. & Parkes, E. (2019). Application of near infra-red reflectance spectroscopy (NIRS) in screening of fresh cassava (Manihot esculenta) storage roots for provitamin A carotenoids. In S.B. Engelsen, K.M. Sørensen and F. van den Berg, Proceedings of the 18th International Conference on Near Infrared Spectroscopy. Chichester, UK: IM Publications, (p. 91-97). |
| spellingShingle | cassava carotenoids carotenes provitamins genotypes near infrared spectroscopy Alamu, Emmanuel Oladeji Maziya-Dixon, B.B. Felde, T. zum Kulakow, Peter A. Parkes, Elizabeth Y. Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title | Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title_full | Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title_fullStr | Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title_full_unstemmed | Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title_short | Application of near infrared reflectance spectroscopy in screening of fresh cassava (Manihot esculenta crantz) storage roots for provitamin A carotenoids |
| title_sort | application of near infrared reflectance spectroscopy in screening of fresh cassava manihot esculenta crantz storage roots for provitamin a carotenoids |
| topic | cassava carotenoids carotenes provitamins genotypes near infrared spectroscopy |
| url | https://hdl.handle.net/10568/101680 |
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