Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.)
Fitotecnia
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| Otros Autores: | |
| Formato: | Tesis |
| Lenguaje: | Inglés |
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Zamorano: Escuela Agrícola Panamericana
2025
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| Acceso en línea: | https://hdl.handle.net/11036/7940 |
| _version_ | 1854968422421495808 |
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| author | Castillo D., Ivanna K. |
| author2 | Avellaneda, Carolina |
| author_browse | Avellaneda, Carolina Castillo D., Ivanna K. |
| author_facet | Avellaneda, Carolina Castillo D., Ivanna K. |
| author_sort | Castillo D., Ivanna K. |
| collection | Biblioteca Digital Zamorano |
| description | Fitotecnia |
| format | Thesis |
| id | ZAMORANO7940 |
| institution | Universidad Zamorano |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Zamorano: Escuela Agrícola Panamericana |
| publisherStr | Zamorano: Escuela Agrícola Panamericana |
| record_format | dspace |
| spelling | ZAMORANO79402025-01-16T15:25:08Z Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) Castillo D., Ivanna K. Avellaneda, Carolina Vogel, Gregory DeJong, Darlene Artificial inoculation Disease resistance Eggplant Plant breeding Solanum aethiopicum Solanum melongena Fitotecnia Verticillium wilt is a significant soilborne disease affecting eggplant (Solanum melongena L.) production worldwide. This study aims to establish a standardized protocol for the artificial inoculation of V. dahliae on eggplant to facilitate disease resistance screening in breeding programs. The research was conducted at the Guterman Greenhouse Complex, Cornell University, and involved multiple experiments to optimize inoculation conditions. Three V. dahliae isolates were tested for pathogenicity on two eggplant cultivars, and the effects of spore concentration, immersion time in the spore suspension, root cutting, and inoculation method (dipping vs. drenching) were evaluated. The study also compared the susceptibility of 24 eggplant varieties, including 12 Solanum aethiopicum varieties. Results indicated significant differences in disease severity and progression based on isolate virulence and interaction between genotype and isolate, as well as some inoculation parameters. It was concluded that 105 conidia mL-1 is a high enough concentration to produce severe symptoms, as well as 5 minutes of immersion in the spore suspension. Cutting the tips of the roots does not influence disease severity when performing artificial inoculations, and dipping the roots causes more severe symptoms in less time than root drenching. All the varieties inoculated were found to be highly susceptible to Verticillium wilt. The established protocol provides a reliable method for screening eggplant germplasm for resistance to Verticillium wilt, contributing to the development of resistant cultivars. 2025-01-16T21:18:06Z 2025-01-16T21:18:06Z 2024 Thesis https://hdl.handle.net/11036/7940 eng Copyright Escuela Agrícola Panamericana, Zamorano https://creativecommons.org/licenses/by-nc-nd/3.0/es/ application/pdf Zamorano: Escuela Agrícola Panamericana |
| spellingShingle | Artificial inoculation Disease resistance Eggplant Plant breeding Solanum aethiopicum Solanum melongena Castillo D., Ivanna K. Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title | Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title_full | Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title_fullStr | Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title_full_unstemmed | Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title_short | Establishing a protocol for the artificial inoculation of Verticillium dahliae on eggplant (Solanum melongena L.) |
| title_sort | establishing a protocol for the artificial inoculation of verticillium dahliae on eggplant solanum melongena l |
| topic | Artificial inoculation Disease resistance Eggplant Plant breeding Solanum aethiopicum Solanum melongena |
| url | https://hdl.handle.net/11036/7940 |
| work_keys_str_mv | AT castillodivannak establishingaprotocolfortheartificialinoculationofverticilliumdahliaeoneggplantsolanummelongenal |