Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies.
Global meat production is expected to increase by 2030, driven by factors such as population growth, income levels, and urbanization with poultry leading the growth due to its short production cycle. Salmonella, a major cause of gastrointestinal illness, is closely linked to poultry contamination, h...
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| Format: | Thesis |
| Language: | Inglés |
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Zamorano: Escuela Agrícola Panamericana
2025
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| Online Access: | https://hdl.handle.net/11036/7836 |
| _version_ | 1854967916760399872 |
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| author | Espinal D., María F. |
| author2 | Luna, Ligia |
| author_browse | Espinal D., María F. Luna, Ligia |
| author_facet | Luna, Ligia Espinal D., María F. |
| author_sort | Espinal D., María F. |
| collection | Biblioteca Digital Zamorano |
| description | Global meat production is expected to increase by 2030, driven by factors such as population growth, income levels, and urbanization with poultry leading the growth due to its short production cycle. Salmonella, a major cause of gastrointestinal illness, is closely linked to poultry contamination, highlighting the need for its control in the industry. This study evaluated the performance of three different chicken sampling methodologies, comparing the traditional Rinsate method with MicroTally® Swab and MicroTally® Mitt, and the evaluation of three quantification methods (GeneUp® Quant Salmonella, Spread Plate, and Spiral Plate) across different concentrations of Salmonella. Chicken breasts were inoculated with four different Salmonella cocktails, and samples were collected using the three sampling methods. The quantification methods included non-incubation and 4-hour incubation protocols for GeneUp® Quant, and XLT4 agar plating using Spread Plate and Spiral Plate techniques. Statistical analyses were performed using SAS software to determine significant differences between methods at each concentration level, providing insights into reliable sampling techniques for detecting and quantifying Salmonella in chicken samples. The study concluded that MicroTally® Mitt was the method more comparable with Rinsate (reference method), which consistently yielded comparable or higher quantifications. While MicroTally® Swab performed similarly behavior, but its results showed more variability. Spread Plating had higher quantifications of bacteria at low concentrations, whereas Spiral Plating at higher concentrations. GeneUp 0 Hours of Incubation had higher quantifications at low concentrations, while GeneUp 4 Hours, at higher concentrations, highlighting the importance of incubation time in molecular quantification. |
| format | Thesis |
| id | ZAMORANO7836 |
| institution | Universidad Zamorano |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Zamorano: Escuela Agrícola Panamericana |
| publisherStr | Zamorano: Escuela Agrícola Panamericana |
| record_format | dspace |
| spelling | ZAMORANO78362025-01-16T15:22:57Z Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. Espinal D., María F. Luna, Ligia Sánchez, Marcos GeneUp Incubation MicroTally® Mitt Rinsate Swab Global meat production is expected to increase by 2030, driven by factors such as population growth, income levels, and urbanization with poultry leading the growth due to its short production cycle. Salmonella, a major cause of gastrointestinal illness, is closely linked to poultry contamination, highlighting the need for its control in the industry. This study evaluated the performance of three different chicken sampling methodologies, comparing the traditional Rinsate method with MicroTally® Swab and MicroTally® Mitt, and the evaluation of three quantification methods (GeneUp® Quant Salmonella, Spread Plate, and Spiral Plate) across different concentrations of Salmonella. Chicken breasts were inoculated with four different Salmonella cocktails, and samples were collected using the three sampling methods. The quantification methods included non-incubation and 4-hour incubation protocols for GeneUp® Quant, and XLT4 agar plating using Spread Plate and Spiral Plate techniques. Statistical analyses were performed using SAS software to determine significant differences between methods at each concentration level, providing insights into reliable sampling techniques for detecting and quantifying Salmonella in chicken samples. The study concluded that MicroTally® Mitt was the method more comparable with Rinsate (reference method), which consistently yielded comparable or higher quantifications. While MicroTally® Swab performed similarly behavior, but its results showed more variability. Spread Plating had higher quantifications of bacteria at low concentrations, whereas Spiral Plating at higher concentrations. GeneUp 0 Hours of Incubation had higher quantifications at low concentrations, while GeneUp 4 Hours, at higher concentrations, highlighting the importance of incubation time in molecular quantification. 2025-01-16T20:28:15Z 2025-01-16T20:28:15Z 2024 Thesis https://hdl.handle.net/11036/7836 eng Copyright Escuela Agrícola Panamericana, Zamorano https://creativecommons.org/licenses/by-nc-nd/3.0/es/ application/pdf Zamorano: Escuela Agrícola Panamericana |
| spellingShingle | GeneUp Incubation MicroTally® Mitt Rinsate Swab Espinal D., María F. Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title | Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title_full | Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title_fullStr | Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title_full_unstemmed | Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title_short | Different Salmonella spp. loads to evaluate the performance of chicken sampling methodologies and quantification methodologies. |
| title_sort | different salmonella spp loads to evaluate the performance of chicken sampling methodologies and quantification methodologies |
| topic | GeneUp Incubation MicroTally® Mitt Rinsate Swab |
| url | https://hdl.handle.net/11036/7836 |
| work_keys_str_mv | AT espinaldmariaf differentsalmonellaspploadstoevaluatetheperformanceofchickensamplingmethodologiesandquantificationmethodologies |