"Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility

This study aimed to research the microbial contamination on beef carcasses in a commercial processing facility using two MicroTally® Mitt procedures for sampling. The primary objective was to evaluate the microbial loads of aerobic counts (AC), Enterobacteriaceae (EB), and Escherichia coli (EC) at v...

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Detalles Bibliográficos
Autor principal: Paz M., Nadia I.
Otros Autores: Acosta, Adela
Formato: Tesis
Lenguaje:Inglés
Publicado: Zamorano: Escuela Agrícola Panamericana 2025
Materias:
Acceso en línea:https://hdl.handle.net/11036/7834
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author Paz M., Nadia I.
author2 Acosta, Adela
author_browse Acosta, Adela
Paz M., Nadia I.
author_facet Acosta, Adela
Paz M., Nadia I.
author_sort Paz M., Nadia I.
collection Biblioteca Digital Zamorano
description This study aimed to research the microbial contamination on beef carcasses in a commercial processing facility using two MicroTally® Mitt procedures for sampling. The primary objective was to evaluate the microbial loads of aerobic counts (AC), Enterobacteriaceae (EB), and Escherichia coli (EC) at various processing stages and compare the effectiveness of dry versus pre-moist sampling methods. Samples were collected from different carcass parts, at different stages and locations including the hide, pre-evisceration, post-evisceration, cold carcass, chuck, trim, and final rail. For the microbial analysis, the TEMPO® system was used for enumeration. The results demonstrated variations in microbial loads across different processing stages and sampling methods. Post-evisceration premoisten bacteria load is significantly difference (P < 0.05) from the dry sampling method for AC, EB, and EC. Moreover, no significant was shown between the treatments in the other stages and locations. However, a significant interaction between treatments and areas was found on AC and EC, indicating that effectiveness varied depending on the area. The investigation demonstrates the presence of the microorganisms at different sample points during the processing line by using MicroTally® Mitt. The findings contribute to improved microbial monitoring and management practices in beef processing facilities to ensure food safety and quality.
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spelling ZAMORANO78342025-01-16T15:22:54Z "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility Paz M., Nadia I. Acosta, Adela Miller, Markus bacterial contamination detection samplings This study aimed to research the microbial contamination on beef carcasses in a commercial processing facility using two MicroTally® Mitt procedures for sampling. The primary objective was to evaluate the microbial loads of aerobic counts (AC), Enterobacteriaceae (EB), and Escherichia coli (EC) at various processing stages and compare the effectiveness of dry versus pre-moist sampling methods. Samples were collected from different carcass parts, at different stages and locations including the hide, pre-evisceration, post-evisceration, cold carcass, chuck, trim, and final rail. For the microbial analysis, the TEMPO® system was used for enumeration. The results demonstrated variations in microbial loads across different processing stages and sampling methods. Post-evisceration premoisten bacteria load is significantly difference (P < 0.05) from the dry sampling method for AC, EB, and EC. Moreover, no significant was shown between the treatments in the other stages and locations. However, a significant interaction between treatments and areas was found on AC and EC, indicating that effectiveness varied depending on the area. The investigation demonstrates the presence of the microorganisms at different sample points during the processing line by using MicroTally® Mitt. The findings contribute to improved microbial monitoring and management practices in beef processing facilities to ensure food safety and quality. 2025-01-16T20:28:15Z 2025-01-16T20:28:15Z 2024 Thesis https://hdl.handle.net/11036/7834 eng Copyright Escuela Agrícola Panamericana, Zamorano https://creativecommons.org/licenses/by-nc-nd/3.0/es/ application/pdf Zamorano: Escuela Agrícola Panamericana
spellingShingle bacterial contamination
detection
samplings
Paz M., Nadia I.
"Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title_full "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title_fullStr "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title_full_unstemmed "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title_short "Assessment of Microbial Indicator Loads and Salmonella spp. prevalence using MicroTally on Beef Carcasses in a Commercial Processing Facility
title_sort assessment of microbial indicator loads and salmonella spp prevalence using microtally on beef carcasses in a commercial processing facility
topic bacterial contamination
detection
samplings
url https://hdl.handle.net/11036/7834
work_keys_str_mv AT pazmnadiai assessmentofmicrobialindicatorloadsandsalmonellasppprevalenceusingmicrotallyonbeefcarcassesinacommercialprocessingfacility