Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials

New trends related to consumers in food packaging include the preference for green and sustainable biopolymers. This study determined a formulation and process flow diagram for the development of an active packaging based on Pullulan and Polylactic acid (PLA), incorporating grape seed and grapefruit...

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Detalles Bibliográficos
Autor principal:	Cerón G., Mirella M.
Otros Autores:	Osorio, Luis F.
Formato:	Tesis
Lenguaje:	Inglés
Publicado:	Zamorano: Escuela Agrícola Panamericana, 2020. 2020
Materias:	Antimicrobial activity Biopolymer Pullulan PLA Physical properties
Acceso en línea:	https://bdigital.zamorano.edu/handle/11036/6932

id	ZAMORANO6932
record_format	dspace
spelling	ZAMORANO69322023-03-24T15:03:52Z Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials Cerón G., Mirella M. Osorio, Luis F. Maldonado, Luis Trinetta, Valentina Antimicrobial activity Biopolymer Pullulan PLA Physical properties New trends related to consumers in food packaging include the preference for green and sustainable biopolymers. This study determined a formulation and process flow diagram for the development of an active packaging based on Pullulan and Polylactic acid (PLA), incorporating grape seed and grapefruit seed extract as antimicrobials. Physical, mechanical, and antimicrobial properties of the created film were evaluated. Two completely randomized design with two variables to optimize film formulations was used. The independent variables were grape seed and grapefruit seed extract in two concentrations (1 and 5%). Three replicates were performed for each treatment, for a total of 24 experimental units for each statistical analysis. Evaluations performed were thickness, ultimate tensile strength (UTS), elongation at break (EL), elasticity (YM), color (L, a, b*), and antimicrobial activity. Results for mechanical properties indicated that there was an interaction between the extract and concentrations, meaning that, depending the concentration of the extract, the mechanical properties will change. In contrast, antimicrobial properties Listeria monocytogenes, Salmonella Infantis and Seftenberg; Escherichia coli O26 and O157:H7 are susceptible to films with grapefruit seed extract. However, the inhibitory effect on bacteria growth will remain the same regardless of concentration. This point out that Pullulan with grapefruit seed extract can be used as an active packaging, inhibiting Gram-positive (Listeria monocytogenes) and Gram-negative (Salmonella spp and Escherichia coli) foodborne pathogens. 2020-12-01T13:50:42Z 2020-12-01T13:50:42Z 2020 Thesis https://bdigital.zamorano.edu/handle/11036/6932 eng 19 p. Copyright Escuela Agrícola Panamericana, Zamorano, 2020 http://creativecommons.org/licenses/by-nc-nd/3.0/es openAccess application/pdf application/pdf Zamorano Zamorano: Escuela Agrícola Panamericana, 2020.
institution	Universidad Zamorano
collection	Biblioteca Digital Zamorano
language	Inglés
topic	Antimicrobial activity Biopolymer Pullulan PLA Physical properties
spellingShingle	Antimicrobial activity Biopolymer Pullulan PLA Physical properties Cerón G., Mirella M. Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
description	New trends related to consumers in food packaging include the preference for green and sustainable biopolymers. This study determined a formulation and process flow diagram for the development of an active packaging based on Pullulan and Polylactic acid (PLA), incorporating grape seed and grapefruit seed extract as antimicrobials. Physical, mechanical, and antimicrobial properties of the created film were evaluated. Two completely randomized design with two variables to optimize film formulations was used. The independent variables were grape seed and grapefruit seed extract in two concentrations (1 and 5%). Three replicates were performed for each treatment, for a total of 24 experimental units for each statistical analysis. Evaluations performed were thickness, ultimate tensile strength (UTS), elongation at break (EL), elasticity (YM), color (L, a, b*), and antimicrobial activity. Results for mechanical properties indicated that there was an interaction between the extract and concentrations, meaning that, depending the concentration of the extract, the mechanical properties will change. In contrast, antimicrobial properties Listeria monocytogenes, Salmonella Infantis and Seftenberg; Escherichia coli O26 and O157:H7 are susceptible to films with grapefruit seed extract. However, the inhibitory effect on bacteria growth will remain the same regardless of concentration. This point out that Pullulan with grapefruit seed extract can be used as an active packaging, inhibiting Gram-positive (Listeria monocytogenes) and Gram-negative (Salmonella spp and Escherichia coli) foodborne pathogens.
author2	Osorio, Luis F.
author_facet	Osorio, Luis F. Cerón G., Mirella M.
format	Tesis
author	Cerón G., Mirella M.
author_sort	Cerón G., Mirella M.
title	Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
title_short	Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
title_full	Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
title_fullStr	Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
title_full_unstemmed	Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
title_sort	development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials
publisher	Zamorano: Escuela Agrícola Panamericana, 2020.
publishDate	2020
url	https://bdigital.zamorano.edu/handle/11036/6932
work_keys_str_mv	AT cerongmirellam developmentofbiobasedactivepackagingmaterialevaluatinggrapefruitseedandgrapeseedextractasantimicrobials
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Development of bio-based active packaging material evaluating grapefruit seed and grape seed extract as antimicrobials

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