Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach

Pythium species are pathogens that cause root rot in hydroponic crops. The objectives of this study were to determine the optimal growth temperature of P. aphanidermatum and P. oopapillum, their pathogenicity in spinach and lettuce, and a suitable method for spinach inoculation. The mycelial growth...

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Detalles Bibliográficos
Autor principal:	Gutierrez Y., Daniela
Otros Autores:	Sierra, Alejandra
Formato:	Tesis
Lenguaje:	Español
Publicado:	Zamorano: Escuela Agrícola Panamericana, 2020 2020
Materias:	Lactuca sativa Root rot Spinacia oleracea Zoospores
Acceso en línea:	https://bdigital.zamorano.edu/handle/11036/6884

id	ZAMORANO6884
record_format	dspace
spelling	ZAMORANO68842023-03-24T15:53:09Z Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach Gutierrez Y., Daniela Sierra, Alejandra Miller, Sally Lactuca sativa Root rot Spinacia oleracea Zoospores Pythium species are pathogens that cause root rot in hydroponic crops. The objectives of this study were to determine the optimal growth temperature of P. aphanidermatum and P. oopapillum, their pathogenicity in spinach and lettuce, and a suitable method for spinach inoculation. The mycelial growth rate of P. aphanidermatum, P. oopapillum 06 and P. oopapillum 07 was determined at 15-20-25-30-35 and 40 °C measuring radial growth every 12 hours for 48 hours. Pathogenicity of these isolates was evaluated in a simulated deep-water culture hydroponic system. Lettuce and spinach inoculations were performed using mycelial infected agar discs and a concentration of 10 zoospores mL-1 was evaluated for spinach. Data collected, at 14 days for lettuce and 16 days for spinach, were root length, fresh and dry root weight, and fresh leaf weight. For spinach, root rot severity was also measured. P. aphanidermatum had the highest growth rate at 25-30 °C and P. oopapillum isolates at 30-35 °C. Inoculated lettuces showed a decrease in all measured variables compared to control. Inoculated spinach had lower fresh leaf, root weight and greater root rot severity than controls. P. aphanidermatum presented the greatest decrease in root length, fresh weight of root and leaves and the highest root rot severity (97.5%) when inoculated with zoospores. Inoculation method with the greatest effect in root length, weight and leaf weight were zoospores. P. aphanidermatum and P. oopapillum affected root development resulting in less plant growth. The use of zoospores is a suitable method of inoculation. 2020-11-30T16:29:24Z 2020-11-30T16:29:24Z 2020 Thesis https://bdigital.zamorano.edu/handle/11036/6884 spa 29 p. Copyright, Escuela Agrícola Panamericana, 2020 http://creativecommons.org/licenses/by-nc-nd/3.0/es openAccess application/pdf Zamorano Zamorano: Escuela Agrícola Panamericana, 2020
institution	Universidad Zamorano
collection	Biblioteca Digital Zamorano
language	Español
topic	Lactuca sativa Root rot Spinacia oleracea Zoospores
spellingShingle	Lactuca sativa Root rot Spinacia oleracea Zoospores Gutierrez Y., Daniela Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
description	Pythium species are pathogens that cause root rot in hydroponic crops. The objectives of this study were to determine the optimal growth temperature of P. aphanidermatum and P. oopapillum, their pathogenicity in spinach and lettuce, and a suitable method for spinach inoculation. The mycelial growth rate of P. aphanidermatum, P. oopapillum 06 and P. oopapillum 07 was determined at 15-20-25-30-35 and 40 °C measuring radial growth every 12 hours for 48 hours. Pathogenicity of these isolates was evaluated in a simulated deep-water culture hydroponic system. Lettuce and spinach inoculations were performed using mycelial infected agar discs and a concentration of 10 zoospores mL-1 was evaluated for spinach. Data collected, at 14 days for lettuce and 16 days for spinach, were root length, fresh and dry root weight, and fresh leaf weight. For spinach, root rot severity was also measured. P. aphanidermatum had the highest growth rate at 25-30 °C and P. oopapillum isolates at 30-35 °C. Inoculated lettuces showed a decrease in all measured variables compared to control. Inoculated spinach had lower fresh leaf, root weight and greater root rot severity than controls. P. aphanidermatum presented the greatest decrease in root length, fresh weight of root and leaves and the highest root rot severity (97.5%) when inoculated with zoospores. Inoculation method with the greatest effect in root length, weight and leaf weight were zoospores. P. aphanidermatum and P. oopapillum affected root development resulting in less plant growth. The use of zoospores is a suitable method of inoculation.
author2	Sierra, Alejandra
author_facet	Sierra, Alejandra Gutierrez Y., Daniela
format	Tesis
author	Gutierrez Y., Daniela
author_sort	Gutierrez Y., Daniela
title	Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
title_short	Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
title_full	Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
title_fullStr	Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
title_full_unstemmed	Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach
title_sort	pathogenicity of pythium aphanidermatum and p. oopapillum in hydroponic lettuce and spinach
publisher	Zamorano: Escuela Agrícola Panamericana, 2020
publishDate	2020
url	https://bdigital.zamorano.edu/handle/11036/6884
work_keys_str_mv	AT gutierrezydaniela pathogenicityofpythiumaphanidermatumandpoopapilluminhydroponiclettuceandspinach
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Pathogenicity of Pythium aphanidermatum and P. oopapillum in hydroponic lettuce and spinach

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