Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka
In order to understand the dynamics of zoonotic disease transmission in the animal-human interface, a One Health approach is imperative. This study investigated the occurrence of the zoonotic pathogens Campylobacter spp., Salmonella spp. and rotavirus in fecal samples from free-ranging endangered to...
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| Formato: | H3 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Clinical Sciences (until 231231)
2016
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| Materias: |
| _version_ | 1855571534420115456 |
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| author | Tegner, Cecilia |
| author_browse | Tegner, Cecilia |
| author_facet | Tegner, Cecilia |
| author_sort | Tegner, Cecilia |
| collection | Epsilon Archive for Student Projects |
| description | In order to understand the dynamics of zoonotic disease transmission in the animal-human interface, a One Health approach is imperative. This study investigated the occurrence of the zoonotic pathogens Campylobacter spp., Salmonella spp. and rotavirus in fecal samples from free-ranging endangered toque macaques (Macaca sinica) and near threatened tufted gray langurs (Semnopithecus priam) in Sri Lanka. During March through May of 2015 samples were opportunistically collected at five sites in Sri Lanka where these primates come into close contact with humans. Standard culturing methods were used to screen for the bacteria and an ELISA-based quick-test was used to detect presence of type A human rotavirus. Bacterial sensitivity to selected antibiotics was analysed using VetMICTM broth microdilution panels.
From the five sites, 98 samples were obtained. All samples tested negative for human type A rotavirus. All 40 samples from gray langurs were negative for Campylobacter spp. and Salmonella spp. Fifty-eight samples were collected from toque macaques, of which ten were positive for C. jejuni, four for C. coli and two for Salmonella Virchow. In vitro resistance to ampicillin, ciprofloxacin, nalidixic acid and tetracycline was detected in C. jejuni samples. All C. coli were in vitro resistant to ampicillin. The detected Salmonella Virchow were sensitive to all the antibiotics tested for.
This study has detected C. jejuni, C. coli and Salmonella Virchow in fecal samples from endangered toque macaques in Sri Lanka with close human contact. The bacteria showed varying sensitivity to antibiotics and several C. jejuni were multidrug resistant. The presence of these bacteria in free-ranging animals could have implications both for non-human primate conservation and public health in Sri Lanka. |
| format | H3 |
| id | RepoSLU9516 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | SLU/Dept. of Clinical Sciences (until 231231) |
| publisherStr | SLU/Dept. of Clinical Sciences (until 231231) |
| record_format | eprints |
| spelling | RepoSLU95162019-06-13T23:15:04Z Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka Potentiellt zoonotiska tarmpatogener hos primater i Sri Lanka Tegner, Cecilia zoonosis salmonella Campylobacter rotavirus non-human primates antibiotic resistance In order to understand the dynamics of zoonotic disease transmission in the animal-human interface, a One Health approach is imperative. This study investigated the occurrence of the zoonotic pathogens Campylobacter spp., Salmonella spp. and rotavirus in fecal samples from free-ranging endangered toque macaques (Macaca sinica) and near threatened tufted gray langurs (Semnopithecus priam) in Sri Lanka. During March through May of 2015 samples were opportunistically collected at five sites in Sri Lanka where these primates come into close contact with humans. Standard culturing methods were used to screen for the bacteria and an ELISA-based quick-test was used to detect presence of type A human rotavirus. Bacterial sensitivity to selected antibiotics was analysed using VetMICTM broth microdilution panels. From the five sites, 98 samples were obtained. All samples tested negative for human type A rotavirus. All 40 samples from gray langurs were negative for Campylobacter spp. and Salmonella spp. Fifty-eight samples were collected from toque macaques, of which ten were positive for C. jejuni, four for C. coli and two for Salmonella Virchow. In vitro resistance to ampicillin, ciprofloxacin, nalidixic acid and tetracycline was detected in C. jejuni samples. All C. coli were in vitro resistant to ampicillin. The detected Salmonella Virchow were sensitive to all the antibiotics tested for. This study has detected C. jejuni, C. coli and Salmonella Virchow in fecal samples from endangered toque macaques in Sri Lanka with close human contact. The bacteria showed varying sensitivity to antibiotics and several C. jejuni were multidrug resistant. The presence of these bacteria in free-ranging animals could have implications both for non-human primate conservation and public health in Sri Lanka. Ett One Health perspektiv är viktigt när epidemiologi hos zoonotiska sjukdomar undersöks – både för bevarande av hotade arter och för folkhälsan. Denna studie undersökte förekomsten av de zoonotiska patogenerna Campylobacter spp., Salmonella spp. och rotavirus i träckprover från den utrotningshotade ceylonmakaken (Macaca sinica) och den nära hotade grå hulmanen (Semnopithecus priam) i Sri Lanka. Prover samlades in opportunistiskt under mars-maj 2015 från fem platser i Sri Lanka där människor och dessa primater kommer i nära kontakt med varandra. Konventionella odlingsmetoder användes för att odla fram bakterier och ett ELISA-baserat snabbtest användes för att detektera humant rotavirus typ A. Bakteriernas känslighet för utvalda antibiotika testades med VetMICTM testpaneler. Från de fem platserna samlades totalt 98 prover in. Alla prov var negativa för humant rotavirus typ A. Alla 40 prover från grå hulmaner var negativa för Campylobacter spp. och Salmonella spp. Totalt samlades 58 prover in från ceylonmakaker och av dessa var tio positiva för C. jejuni, fyra för C. coli och två för Salmonella Virchow. In vitro resistens mot ampicillin, ciprofloxacin, nalidixinsyra och tetracyklin påvisades hos C. jejuni. Alla C. coli var in vitro resistenta mot ampicillin. De två detekterade Salmonella Virchow var känsliga mot alla undersökta antibiotika. Denna studie har detekterat C. jejuni, C. coli och Salmonella Virchow i träckprover från utrotningshotade ceylonmakaker i Sri Lanka med nära kontakt med människor. Bakterierna uppvisade varierande känslighet mot antibiotika och flera C. jejuni var multidrogresistenta. Detektionen av dessa bakterier hos vilda djur kan ha konsekvenser både för bevarande av icke-mänskliga primater och folkhälsan i Sri Lanka. SLU/Dept. of Clinical Sciences (until 231231) 2016 H3 eng swe https://stud.epsilon.slu.se/9516/ |
| spellingShingle | zoonosis salmonella Campylobacter rotavirus non-human primates antibiotic resistance Tegner, Cecilia Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title | Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title_full | Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title_fullStr | Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title_full_unstemmed | Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title_short | Enteric pathogens of zoonotic concern in selected non-human primates in Sri Lanka |
| title_sort | enteric pathogens of zoonotic concern in selected non-human primates in sri lanka |
| topic | zoonosis salmonella Campylobacter rotavirus non-human primates antibiotic resistance |