The effect of differential phosphorylation of YB-1 on apoptosis and cell cycle regulation
The purpose of this study was to investigate whether different phosphorylated forms of Y-box binding protein (YB-1) can affect the regulation of the cell cycle and/or apoptosis of cancer cells. Recent studies have suggested a key role for YB-1 in the regulation of cancer. Just like many other oncop...
| Autor principal: | |
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2016
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| Acceso en línea: | https://stud.epsilon.slu.se/8799/ |
| _version_ | 1855571409715068928 |
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| author | Hultman, Tove |
| author_browse | Hultman, Tove |
| author_facet | Hultman, Tove |
| author_sort | Hultman, Tove |
| collection | Epsilon Archive for Student Projects |
| description | The purpose of this study was to investigate whether different phosphorylated forms of Y-box binding protein (YB-1) can affect the regulation of the cell cycle and/or apoptosis of cancer cells. Recent studies have suggested a key role for YB-1 in the regulation of cancer.
Just like many other oncoproteins YB-1 is required for vital processes in the cell and hence it is not possible to apply YB-1 in a therapeutic role. Therefore, my aim was to elucidate whether or not one or more phosphorylated forms of YB-1 play a decisive role in apoptosis.
Human colorectal cancer cells (HCT 116) were transfected with different phosphorylated forms of YB-1. These cells were subsequently analysed by flow cytometry in order to compare live/dead cells between the different mutants.
The results from this study suggest that one of the mutants (S176A) plays a more important role when it comes to protecting the cells from apoptosis and hence contribute to promoting sustained proliferation. Moreover, in opposite to S176A, the results indicate that another mutant (S167A) seems to have a more important role protecting the cells from sustained proliferation. |
| format | Second cycle, A2E |
| id | RepoSLU8799 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2016 |
| publishDateSort | 2016 |
| record_format | eprints |
| spelling | RepoSLU87992016-02-15T10:43:24Z https://stud.epsilon.slu.se/8799/ The effect of differential phosphorylation of YB-1 on apoptosis and cell cycle regulation Hultman, Tove Animal physiology and biochemistry Animal diseases The purpose of this study was to investigate whether different phosphorylated forms of Y-box binding protein (YB-1) can affect the regulation of the cell cycle and/or apoptosis of cancer cells. Recent studies have suggested a key role for YB-1 in the regulation of cancer. Just like many other oncoproteins YB-1 is required for vital processes in the cell and hence it is not possible to apply YB-1 in a therapeutic role. Therefore, my aim was to elucidate whether or not one or more phosphorylated forms of YB-1 play a decisive role in apoptosis. Human colorectal cancer cells (HCT 116) were transfected with different phosphorylated forms of YB-1. These cells were subsequently analysed by flow cytometry in order to compare live/dead cells between the different mutants. The results from this study suggest that one of the mutants (S176A) plays a more important role when it comes to protecting the cells from apoptosis and hence contribute to promoting sustained proliferation. Moreover, in opposite to S176A, the results indicate that another mutant (S167A) seems to have a more important role protecting the cells from sustained proliferation. Syftet med denna studie var att undersöka huruvida olika fosforylerade former av Y-box binding protein-1 (YB-1) påverkar celldöd och/eller reglering av cell cykeln. Tidigare studier tyder på att YB-1 har en nyckelposition gällande reglering och uppkomst av cancer. Precis som med många andra onkoproteiner krävs YB-1 för många vitala processer i cellerna och därför kan inte behandling riktas generellt mot YB-1. Med denna studie hoppas jag därför kunna finna en eller flera olika fosforylerade former av YB-1 som har en mer eller mindre avgörande roll gällande apoptos. Detta för att behandling av cancer i framtiden då skulle kunna riktas mot enbart ett eller flera av dessa fosforyleringsställen. För att undersöka detta har humana colo-rektala cancerceller transfekterats med sex olika mutationer av YB-1, dessa har sedan analyserats med hjälp av flödescytometri för att jämföra levande/döda celler mellan de olika mutationerna. 2016-02-10 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/8799/11/hultman_t_160212.pdf Hultman, Tove, 2016. The effect of differential phosphorylation of YB-1 on apoptosis and cell cycle regulation. Second cycle, A2E. Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-5125 eng |
| spellingShingle | Animal physiology and biochemistry Animal diseases Hultman, Tove The effect of differential phosphorylation of YB-1 on apoptosis and cell cycle regulation |
| title | The effect of differential phosphorylation of
YB-1 on apoptosis and cell cycle regulation |
| title_full | The effect of differential phosphorylation of
YB-1 on apoptosis and cell cycle regulation |
| title_fullStr | The effect of differential phosphorylation of
YB-1 on apoptosis and cell cycle regulation |
| title_full_unstemmed | The effect of differential phosphorylation of
YB-1 on apoptosis and cell cycle regulation |
| title_short | The effect of differential phosphorylation of
YB-1 on apoptosis and cell cycle regulation |
| title_sort | effect of differential phosphorylation of
yb-1 on apoptosis and cell cycle regulation |
| topic | Animal physiology and biochemistry Animal diseases |
| url | https://stud.epsilon.slu.se/8799/ https://stud.epsilon.slu.se/8799/ |