Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica
BACKGROUND: Crambe is an oil-crop suitable for industrial purposes and breeding aiming at producing novel oil-qualities may be achieved by genetransformation. Understanding the synthesis of fatty acids and an efficient transformation protocol is of pivotal role in this context. Two transformation...
| Autor principal: | |
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| Formato: | H2 |
| Lenguaje: | Inglés |
| Publicado: |
SLU/Dept. of Plant Breeding (from 130101)
2015
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| Materias: |
| _version_ | 1855571242553180160 |
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| author | Jeppsson, Simon |
| author_browse | Jeppsson, Simon |
| author_facet | Jeppsson, Simon |
| author_sort | Jeppsson, Simon |
| collection | Epsilon Archive for Student Projects |
| description | BACKGROUND: Crambe is an oil-crop suitable for industrial purposes and breeding aiming at producing novel oil-qualities may be achieved by genetransformation.
Understanding the synthesis of fatty acids and an efficient
transformation protocol is of pivotal role in this context.
Two transformation protocols (Agrobacterium mediated according Bang-Quang Huang and oral dip according Clough and Bent 1998) for crambe are evaluated, and a RNAi construct is produced aiming to down-regulate CaDGAT1.
RESULTS: None of the evaluated transformation protocols produce any successfully transformed plants during this evaluation. CaDGAT1 is partially sequenced and a RNAi construct for CaDGAT is produced, while the functionality
of the construct is un-validated.
CONCLUSIONS: Alternative Agrobacterium mediated transformation protocols published by Li et al. 2010 have managed to transform crambe successfully but a functional
oral dip protocol may be even more cost effective.
more studies on oral dip must be carried out to evaluate this however. |
| format | H2 |
| id | RepoSLU7782 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | SLU/Dept. of Plant Breeding (from 130101) |
| publisherStr | SLU/Dept. of Plant Breeding (from 130101) |
| record_format | eprints |
| spelling | RepoSLU77822015-04-07T09:09:35Z Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica Evaluering av två transformations protokoll för Crambe hispanica subsp. abyssinica Jeppsson, Simon Crambe transformation CaDGAT1 floral dip industrial oil crop tailored oil quality BACKGROUND: Crambe is an oil-crop suitable for industrial purposes and breeding aiming at producing novel oil-qualities may be achieved by genetransformation. Understanding the synthesis of fatty acids and an efficient transformation protocol is of pivotal role in this context. Two transformation protocols (Agrobacterium mediated according Bang-Quang Huang and oral dip according Clough and Bent 1998) for crambe are evaluated, and a RNAi construct is produced aiming to down-regulate CaDGAT1. RESULTS: None of the evaluated transformation protocols produce any successfully transformed plants during this evaluation. CaDGAT1 is partially sequenced and a RNAi construct for CaDGAT is produced, while the functionality of the construct is un-validated. CONCLUSIONS: Alternative Agrobacterium mediated transformation protocols published by Li et al. 2010 have managed to transform crambe successfully but a functional oral dip protocol may be even more cost effective. more studies on oral dip must be carried out to evaluate this however. SLU/Dept. of Plant Breeding (from 130101) 2015 H2 eng https://stud.epsilon.slu.se/7782/ |
| spellingShingle | Crambe transformation CaDGAT1 floral dip industrial oil crop tailored oil quality Jeppsson, Simon Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title | Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title_full | Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title_fullStr | Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title_full_unstemmed | Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title_short | Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica |
| title_sort | evaluation of two transformation protocols for crambe hispanica subsp. abyssinica |
| topic | Crambe transformation CaDGAT1 floral dip industrial oil crop tailored oil quality |