Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus
European Ash is one of the economically important tree in Europe which is threatened by an invasive pathogen namely Hymenoscyphus pseudoalbidus, causing Ash dieback disease. Our objective was to investigate genes that are expressed after inoculation with this fungus and with freezing injury. In this...
| Autor principal: | |
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| Formato: | Second cycle, A1E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2015
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| Acceso en línea: | https://stud.epsilon.slu.se/7604/ |
| _version_ | 1855571212811370496 |
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| author | Eshghi Sahraei, Shadi |
| author_browse | Eshghi Sahraei, Shadi |
| author_facet | Eshghi Sahraei, Shadi |
| author_sort | Eshghi Sahraei, Shadi |
| collection | Epsilon Archive for Student Projects |
| description | European Ash is one of the economically important tree in Europe which is threatened by an invasive pathogen namely Hymenoscyphus pseudoalbidus, causing Ash dieback disease. Our objective was to investigate genes that are expressed after inoculation with this fungus and with freezing injury. In this experiment, high-throughput cDNA sequencing (RNA-seq) data was obtained from Ash bark subjected to different experimental conditions namely control, freezing and inoculation with fungus. RNA-seq data were generated by Illumina sequencing and analyzed by TopHat and Cufflinks. 70083 transcripts were represented in the libraries which all of them belonged to Ash. 97 transcripts were significantly differentially expressed in the control and freezing comparisons, 204 transcripts in the control and inoculation comparison and 151 transcripts in the comparison between freezing and inoculation. It was found that genes responded to abscisic acid (ABA) were up regulated by inoculation. But the expression of ABA 8-hydroxylase genes, which play roles in ABA degradation, was decreased. |
| format | Second cycle, A1E |
| id | RepoSLU7604 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2015 |
| publishDateSort | 2015 |
| record_format | eprints |
| spelling | RepoSLU76042015-02-12T13:36:50Z https://stud.epsilon.slu.se/7604/ Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus Eshghi Sahraei, Shadi Plant diseases European Ash is one of the economically important tree in Europe which is threatened by an invasive pathogen namely Hymenoscyphus pseudoalbidus, causing Ash dieback disease. Our objective was to investigate genes that are expressed after inoculation with this fungus and with freezing injury. In this experiment, high-throughput cDNA sequencing (RNA-seq) data was obtained from Ash bark subjected to different experimental conditions namely control, freezing and inoculation with fungus. RNA-seq data were generated by Illumina sequencing and analyzed by TopHat and Cufflinks. 70083 transcripts were represented in the libraries which all of them belonged to Ash. 97 transcripts were significantly differentially expressed in the control and freezing comparisons, 204 transcripts in the control and inoculation comparison and 151 transcripts in the comparison between freezing and inoculation. It was found that genes responded to abscisic acid (ABA) were up regulated by inoculation. But the expression of ABA 8-hydroxylase genes, which play roles in ABA degradation, was decreased. 2015-02-10 Second cycle, A1E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/7604/8/eshghi_sahraei_s_150210.pdf Eshghi Sahraei, Shadi, 2015. Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus. Second cycle, A1E. Uppsala: (NL, NJ) > Dept. of Forest Mycology and Plant Pathology <https://stud.epsilon.slu.se/view/divisions/OID-390.html> urn:nbn:se:slu:epsilon-s-4103 eng |
| spellingShingle | Plant diseases Eshghi Sahraei, Shadi Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title | Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title_full | Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title_fullStr | Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title_full_unstemmed | Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title_short | Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus |
| title_sort | gene expression in european ash in response to inoculation with hymenoscyphus pseudoalbidus |
| topic | Plant diseases |
| url | https://stud.epsilon.slu.se/7604/ https://stud.epsilon.slu.se/7604/ |