Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa
Morphological characterisation of germplasm helps in the identification of duplicate accessions, unique traits and the development of core collection. Cryopreservation serves as a complementary conservation method to field and in-vitro methods. This study aimed at evaluating the diversity of Ipomoea...
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| Formato: | H2 |
| Lenguaje: | Inglés |
| Publicado: |
SLU/Swedish Biodiversity Centre
2009
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| Materias: | |
| Acceso en línea: | http://www.cbm.slu.se/eng/mastersprog/thesis2009/Nr%2068%20Tshidi%20Manamela.pdf |
| _version_ | 1855570211268198400 |
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| author | Manamela, Machoene Tshidi |
| author_browse | Manamela, Machoene Tshidi |
| author_facet | Manamela, Machoene Tshidi |
| author_sort | Manamela, Machoene Tshidi |
| collection | Epsilon Archive for Student Projects |
| description | Morphological characterisation of germplasm helps in the identification of duplicate accessions, unique traits and the development of core collection. Cryopreservation serves as a complementary conservation method to field and in-vitro methods. This study aimed at evaluating the diversity of Ipomoea batatas landrace collection maintained in tissue culture at the National Plant Genetic Resources Centre (NPGRC) of South Africa. The response of the selected sweet potato accessions to cryopreservation as the complementary conservation method was also tested. Vegetative and storage root characters of the 51 sweet potato accessions were characterized using International Potato Centre descriptors for sweet potato and analyzed with Numerical Taxonomy System-pc software. Encapsulation-vitrification and encapsulation-dehydration protocols were tested for cryopreservation on selected accessions. The unweighted pair-group method using an arithmetic average (UPGMA) defined 22 clusters at a distance coefficient of approximately one. Four Principal Components cumulatively explained 46 percent of the variation, whereas four Principal Coordinate axes, explained 50 percent. Preliminary results presented here show that the NPGRC of South Africa conserves high morphological diversity of sweet potato landraces in the field and tissue culture. But, the results need to be verified with replicates. |
| format | H2 |
| id | RepoSLU653 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Swedish Biodiversity Centre |
| publisherStr | SLU/Swedish Biodiversity Centre |
| record_format | eprints |
| spelling | RepoSLU6532012-04-20T14:10:24Z Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa Manamela, Machoene Tshidi cryopreservation duplicate accessions germplasm morphological characterisation NPGRC phenotypic diversity sweet potato South Africa Morphological characterisation of germplasm helps in the identification of duplicate accessions, unique traits and the development of core collection. Cryopreservation serves as a complementary conservation method to field and in-vitro methods. This study aimed at evaluating the diversity of Ipomoea batatas landrace collection maintained in tissue culture at the National Plant Genetic Resources Centre (NPGRC) of South Africa. The response of the selected sweet potato accessions to cryopreservation as the complementary conservation method was also tested. Vegetative and storage root characters of the 51 sweet potato accessions were characterized using International Potato Centre descriptors for sweet potato and analyzed with Numerical Taxonomy System-pc software. Encapsulation-vitrification and encapsulation-dehydration protocols were tested for cryopreservation on selected accessions. The unweighted pair-group method using an arithmetic average (UPGMA) defined 22 clusters at a distance coefficient of approximately one. Four Principal Components cumulatively explained 46 percent of the variation, whereas four Principal Coordinate axes, explained 50 percent. Preliminary results presented here show that the NPGRC of South Africa conserves high morphological diversity of sweet potato landraces in the field and tissue culture. But, the results need to be verified with replicates. SLU/Swedish Biodiversity Centre 2009 H2 eng http://www.cbm.slu.se/eng/mastersprog/thesis2009/Nr%2068%20Tshidi%20Manamela.pdf https://stud.epsilon.slu.se/653/ |
| spellingShingle | cryopreservation duplicate accessions germplasm morphological characterisation NPGRC phenotypic diversity sweet potato South Africa Manamela, Machoene Tshidi Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title | Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title_full | Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title_fullStr | Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title_full_unstemmed | Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title_short | Morphological characterisation and cryopreservation of sweet potato, Ipomoea batatas (L.) Lam., accessions at the NPGRC of South Africa |
| title_sort | morphological characterisation and cryopreservation of sweet potato, ipomoea batatas (l.) lam., accessions at the npgrc of south africa |
| topic | cryopreservation duplicate accessions germplasm morphological characterisation NPGRC phenotypic diversity sweet potato South Africa |
| url | http://www.cbm.slu.se/eng/mastersprog/thesis2009/Nr%2068%20Tshidi%20Manamela.pdf |