Blood compatibility of biomaterials made of recombinant spider silk
Native spider silk is an attractive lightweight natural polymeric material with a unique combination of strength, extendibility and flexibility. Synthetic, recombinant spider silk is an attractive biomaterial with several possible fields of application. When a foreign material is introduced to bloo...
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| Formato: | H2 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231)
2013
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| Materias: |
| _version_ | 1855570875055603712 |
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| author | Segall, Michael |
| author_browse | Segall, Michael |
| author_facet | Segall, Michael |
| author_sort | Segall, Michael |
| collection | Epsilon Archive for Student Projects |
| description | Native spider silk is an attractive lightweight natural polymeric material with a unique combination of strength, extendibility and flexibility. Synthetic, recombinant spider silk is an attractive biomaterial with several possible fields of application.
When a foreign material is introduced to blood, this can for example cause unwanted coagulation and complement activation.
The aim of this study was to investigate if coatings of heparin or albumin could improve the blood compatibility of films of recombinant spider silk and to develop an initial method to do so. Hypothetically, objects like pacemakers, stents and dental implants could be covered with spider silk films of high blood compatibility to reduce potentially harmful foreign body reactions caused by the immune defense. Increased blood compatibility would likely lead to improvements for both the individual patients and the society thanks to reduced load on healthcare institutions.
Our objective was to assess the blood compatibility of both functionalized and non-functionalized recombinant spider silk by evaluating coagulation and activation of the complement system of the immune defense.
Coagulation was evaluated visually on glass slides and complement activation was analyzed by measuring the concentration of the complement system mediator C3a in blood samples that had been exposed to the different materials.
This study introduces a non-toxic and easy performed method of improving the blood compatibility of recombinant spider silk films by adding a heparin coating.
Heparin coated surfaces had statistically significantly higher blood compatibility than uncoated surfaces. The effects of albumin coating of films were less clear compared to the results of heparin coated films.
Complement activation could not be evaluated because of high activation in all samples prior contact with the materials that were included in the study. |
| format | H2 |
| id | RepoSLU5618 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| publisherStr | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| record_format | eprints |
| spelling | RepoSLU56182013-05-29T10:15:31Z Blood compatibility of biomaterials made of recombinant spider silk Blodkompatibilitet hos biomaterial av rekombinant spindeltråd Segall, Michael recombinant spider silk biomaterials blood compatibility heparin albumin 4RepCT coating rekombinant spindeltråd biomaterial blodkompatibilitet beläggning Native spider silk is an attractive lightweight natural polymeric material with a unique combination of strength, extendibility and flexibility. Synthetic, recombinant spider silk is an attractive biomaterial with several possible fields of application. When a foreign material is introduced to blood, this can for example cause unwanted coagulation and complement activation. The aim of this study was to investigate if coatings of heparin or albumin could improve the blood compatibility of films of recombinant spider silk and to develop an initial method to do so. Hypothetically, objects like pacemakers, stents and dental implants could be covered with spider silk films of high blood compatibility to reduce potentially harmful foreign body reactions caused by the immune defense. Increased blood compatibility would likely lead to improvements for both the individual patients and the society thanks to reduced load on healthcare institutions. Our objective was to assess the blood compatibility of both functionalized and non-functionalized recombinant spider silk by evaluating coagulation and activation of the complement system of the immune defense. Coagulation was evaluated visually on glass slides and complement activation was analyzed by measuring the concentration of the complement system mediator C3a in blood samples that had been exposed to the different materials. This study introduces a non-toxic and easy performed method of improving the blood compatibility of recombinant spider silk films by adding a heparin coating. Heparin coated surfaces had statistically significantly higher blood compatibility than uncoated surfaces. The effects of albumin coating of films were less clear compared to the results of heparin coated films. Complement activation could not be evaluated because of high activation in all samples prior contact with the materials that were included in the study. Naturlig spindeltråd är ett lätt material som är både flexibelt, elastiskt och starkt. Syntetisk rekombinant spindeltråd har flera möjliga användningsområden som biomaterial. När ett främmande material kommer i kontakt med blod kan det leda till ogynnsam koagulation och komplementaktivering. Syftet med den här studien var att undersöka om blodkompatibiliteten på filmer av rekombinant spindeltråd kunde förbättras med hjälp av beläggningar av heparin eller albumin som anlagts med enkla och giftfria metoder. Hypotetiskt skulle biomaterial och implantat kunna täckas av blodkompatibla filmer för att minska kroppens reaktion mot det som inte är kroppseget och på så sätt leda till ökat välbefinnande för den enskilda patienten och även för samhället i stort genom minskat vårdbehov. Vårt mål var att undersöka blodkompatibiliteten hos både rekombinant spindeltråd med extra egenskaper och vanlig spindeltråd genom att övervaka aktiveringen av koagulationsprocessen och komplementsystemet. Koagulationspåverkan studerades på objektsglas och komplementaktiveringen undersöktes genom att analysera koncentrationen av komplementfaktorn C3a. Här presenteras en icke-toxisk och enkel metod för att öka blodkompatibiliteten hos spindeltrådsfilmer genom att belägga dessa med heparin. Albuminbeläggningens effekt på blodkompatibiliteten var mindre tydliga än effekten av heparinbeläggningarna, vars effekt gick att säkerställa statistiskt. Aktiveringen av komplementsystemet kunde inte utvärderas på grund av för hög aktivitetsnivå i alla prover som analyserades. SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) 2013 H2 eng swe https://stud.epsilon.slu.se/5618/ |
| spellingShingle | recombinant spider silk biomaterials blood compatibility heparin albumin 4RepCT coating rekombinant spindeltråd biomaterial blodkompatibilitet beläggning Segall, Michael Blood compatibility of biomaterials made of recombinant spider silk |
| title | Blood compatibility of biomaterials made of recombinant spider silk |
| title_full | Blood compatibility of biomaterials made of recombinant spider silk |
| title_fullStr | Blood compatibility of biomaterials made of recombinant spider silk |
| title_full_unstemmed | Blood compatibility of biomaterials made of recombinant spider silk |
| title_short | Blood compatibility of biomaterials made of recombinant spider silk |
| title_sort | blood compatibility of biomaterials made of recombinant spider silk |
| topic | recombinant spider silk biomaterials blood compatibility heparin albumin 4RepCT coating rekombinant spindeltråd biomaterial blodkompatibilitet beläggning |