Modification of the plant retinoblastoma-related protein (RBR1) by SUMO : structural and functional studies
The retinoblastoma protein (Rb) is a transcription regulator and key component of the Rb/E2F/DP pathway which regulates progression of the cell cycle in plants and animals. Within the pathway, Rb blocks E2F transcriptional activity consequently ensuring restricted cell proliferation. Of great import...
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| Format: | H2 |
| Language: | Inglés |
| Published: |
SLU/Dept. of Forest Genetics and Plant Physiology
2009
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| Subjects: |
| _version_ | 1855570201590890496 |
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| author | Businge, Edward |
| author_browse | Businge, Edward |
| author_facet | Businge, Edward |
| author_sort | Businge, Edward |
| collection | Epsilon Archive for Student Projects |
| description | The retinoblastoma protein (Rb) is a transcription regulator and key component of the
Rb/E2F/DP pathway which regulates progression of the cell cycle in plants and animals.
Within the pathway, Rb blocks E2F transcriptional activity consequently ensuring
restricted cell proliferation. Of great importance too, is a family of posttranslational
modifiers referred to as small ubiquitin-related modifiers (SUMO), whose modification
consequences include; sub cellular localization of proteins, alteration of protein to protein
interaction and regulation of transcriptional activity.
In order to study and depict the plant retinoblastoma related protein (RBR1) as a SUMO
substrate; its modification site was mutated to address the effect of the mutation on
protein localization. Additionally, an in-vitro assay was used to further illustrate the
consequences of the mutation. In protoplasts transfected with wild type RBR1 the protein
was solely present in the nucleus while those transfected with mutated RBR1, the protein
was seen in both the nucleus and the cytosol. From the in vitro SUMOylation assay it was
evident that while wild type RBR1 could be modified by SUMO, its mutated version
could not undergo modification.
The results from this study don’t only show RBR1 as a SUMO substrate; they also
suggest that modification by SUMO could be needed for its sub-cellular localization. |
| format | H2 |
| id | RepoSLU555 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Dept. of Forest Genetics and Plant Physiology |
| publisherStr | SLU/Dept. of Forest Genetics and Plant Physiology |
| record_format | eprints |
| spelling | RepoSLU5552012-04-20T14:10:04Z Modification of the plant retinoblastoma-related protein (RBR1) by SUMO : structural and functional studies Businge, Edward SUMO Retinoblastoma Plant protein Arabidopsis The retinoblastoma protein (Rb) is a transcription regulator and key component of the Rb/E2F/DP pathway which regulates progression of the cell cycle in plants and animals. Within the pathway, Rb blocks E2F transcriptional activity consequently ensuring restricted cell proliferation. Of great importance too, is a family of posttranslational modifiers referred to as small ubiquitin-related modifiers (SUMO), whose modification consequences include; sub cellular localization of proteins, alteration of protein to protein interaction and regulation of transcriptional activity. In order to study and depict the plant retinoblastoma related protein (RBR1) as a SUMO substrate; its modification site was mutated to address the effect of the mutation on protein localization. Additionally, an in-vitro assay was used to further illustrate the consequences of the mutation. In protoplasts transfected with wild type RBR1 the protein was solely present in the nucleus while those transfected with mutated RBR1, the protein was seen in both the nucleus and the cytosol. From the in vitro SUMOylation assay it was evident that while wild type RBR1 could be modified by SUMO, its mutated version could not undergo modification. The results from this study don’t only show RBR1 as a SUMO substrate; they also suggest that modification by SUMO could be needed for its sub-cellular localization. SLU/Dept. of Forest Genetics and Plant Physiology 2009 H2 eng https://stud.epsilon.slu.se/555/ |
| spellingShingle | SUMO Retinoblastoma Plant protein Arabidopsis Businge, Edward Modification of the plant retinoblastoma-related protein (RBR1) by SUMO : structural and functional studies |
| title | Modification of the plant retinoblastoma-related protein
(RBR1) by SUMO : structural and functional studies |
| title_full | Modification of the plant retinoblastoma-related protein
(RBR1) by SUMO : structural and functional studies |
| title_fullStr | Modification of the plant retinoblastoma-related protein
(RBR1) by SUMO : structural and functional studies |
| title_full_unstemmed | Modification of the plant retinoblastoma-related protein
(RBR1) by SUMO : structural and functional studies |
| title_short | Modification of the plant retinoblastoma-related protein
(RBR1) by SUMO : structural and functional studies |
| title_sort | modification of the plant retinoblastoma-related protein
(rbr1) by sumo : structural and functional studies |
| topic | SUMO Retinoblastoma Plant protein Arabidopsis |