Comparative genomics : understanding regulation of hydrogenases in the nitrogen-fixing Frankia
Frankia is a facultative symbiont actinomycete living with the dicotyledonous plant worldwide. Two hydrogenase functions- uptake hydrogenase and hydrogenevolving hydrogenase- have been so far reported in Frankia species, while the presence of [Fe] hydrogenase function was surmised. Investigation of...
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| Formato: | H2 |
| Lenguaje: | Inglés |
| Publicado: |
SLU/Dept. of Forest Genetics and Plant Physiology
2009
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| Materias: |
| _version_ | 1855570201389563904 |
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| author | Kosawang, Chatchai |
| author_browse | Kosawang, Chatchai |
| author_facet | Kosawang, Chatchai |
| author_sort | Kosawang, Chatchai |
| collection | Epsilon Archive for Student Projects |
| description | Frankia is a facultative symbiont actinomycete living with the dicotyledonous
plant worldwide. Two hydrogenase functions- uptake hydrogenase and hydrogenevolving
hydrogenase- have been so far reported in Frankia species, while the
presence of [Fe] hydrogenase function was surmised. Investigation of three Frankia
strains, ACN14a, CcI3 and R43 disclosed non-existence of the [Fe] hydrogenase
function, and bidirectional hydrogenase function in two of the Frankia strains,
ACN14a and CcI3. The blast of the three available Frankia genomes with the
conserved region of [Fe] hydrogenase, subunits of cyanobacterial bidirectional
hydrogenase, hyd and hyn genes resulted in no relevant information, but uptake
hydrogenase. Regarding the gene expression studies, it is supposed that [Fe]
hydrogenase is unlikely to presence in the Frankia strains. On the other hand,
bidirectional hydrogenase function is unlikely to occur in the strains ACN14a and
CcI3 since hydrogen evolution was not detected in these strains. The hydrogen
production in the strain R43 was found both under aerobic and anaerobic condition
regardless of nickel availability. Application of hox-specific primers to all the strains
evaluated did not provide an informative dataset. It is possible that hydrogen
evolution in the strain R43 was retrieved from uptake hydrogenase function acting in
reverse direction. In addition, it was shown that uptake hydrogenase was
consecutively expressed. However, knowledge about hydrogenase in Frankia is now
like the tip of the iceberg. It is not possible to show the presence of bidirectional
hydrogenase gene in the strain R43 until its genome is sequenced. |
| format | H2 |
| id | RepoSLU554 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Dept. of Forest Genetics and Plant Physiology |
| publisherStr | SLU/Dept. of Forest Genetics and Plant Physiology |
| record_format | eprints |
| spelling | RepoSLU5542012-04-20T14:10:04Z Comparative genomics : understanding regulation of hydrogenases in the nitrogen-fixing Frankia Kosawang, Chatchai Genomics Hydrogenases Nitrogen fixation Frankia Frankia is a facultative symbiont actinomycete living with the dicotyledonous plant worldwide. Two hydrogenase functions- uptake hydrogenase and hydrogenevolving hydrogenase- have been so far reported in Frankia species, while the presence of [Fe] hydrogenase function was surmised. Investigation of three Frankia strains, ACN14a, CcI3 and R43 disclosed non-existence of the [Fe] hydrogenase function, and bidirectional hydrogenase function in two of the Frankia strains, ACN14a and CcI3. The blast of the three available Frankia genomes with the conserved region of [Fe] hydrogenase, subunits of cyanobacterial bidirectional hydrogenase, hyd and hyn genes resulted in no relevant information, but uptake hydrogenase. Regarding the gene expression studies, it is supposed that [Fe] hydrogenase is unlikely to presence in the Frankia strains. On the other hand, bidirectional hydrogenase function is unlikely to occur in the strains ACN14a and CcI3 since hydrogen evolution was not detected in these strains. The hydrogen production in the strain R43 was found both under aerobic and anaerobic condition regardless of nickel availability. Application of hox-specific primers to all the strains evaluated did not provide an informative dataset. It is possible that hydrogen evolution in the strain R43 was retrieved from uptake hydrogenase function acting in reverse direction. In addition, it was shown that uptake hydrogenase was consecutively expressed. However, knowledge about hydrogenase in Frankia is now like the tip of the iceberg. It is not possible to show the presence of bidirectional hydrogenase gene in the strain R43 until its genome is sequenced. SLU/Dept. of Forest Genetics and Plant Physiology 2009 H2 eng https://stud.epsilon.slu.se/554/ |
| spellingShingle | Genomics Hydrogenases Nitrogen fixation Frankia Kosawang, Chatchai Comparative genomics : understanding regulation of hydrogenases in the nitrogen-fixing Frankia |
| title | Comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing Frankia |
| title_full | Comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing Frankia |
| title_fullStr | Comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing Frankia |
| title_full_unstemmed | Comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing Frankia |
| title_short | Comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing Frankia |
| title_sort | comparative genomics : understanding regulation of
hydrogenases in the nitrogen-fixing frankia |
| topic | Genomics Hydrogenases Nitrogen fixation Frankia |