Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil
Rotavirus and coronavirus are the two most common viral causes of neonatal calf diarrhea and their presence causes a lot of economic damage to the farmers as well as suffering to the animal. By getting better knowledge about the viruses we may help in tracing transmission and in producing new vaccin...
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| Formato: | H2 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2013
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| Materias: |
| _version_ | 1855570809364414464 |
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| author | Jakobsson, Samuel |
| author_browse | Jakobsson, Samuel |
| author_facet | Jakobsson, Samuel |
| author_sort | Jakobsson, Samuel |
| collection | Epsilon Archive for Student Projects |
| description | Rotavirus and coronavirus are the two most common viral causes of neonatal calf diarrhea and their presence causes a lot of economic damage to the farmers as well as suffering to the animal. By getting better knowledge about the viruses we may help in tracing transmission and in producing new vaccines. This paper served to study the molecular epidemiology of rotavirus and coronavirus in fecal samples collected from two different farms in the state of São Paulo, Brazil. These samples have been studied with focus on the gene VP4, VP7, NSP4 in rotavirus and the gene S1 in coronavirus. From the first farm the screening PCR showed that 12/48 samples were positive for rotavirus and 4/48 positive for coronavirus. No determination of genotypes of rotavirus was achieved and the sequences retrieved did not match any known rotavirus. Nor was any amplification and sequencing of the positive samples of coronavirus successful. From the second farm 14/22 samples tested positive for rotavirus and 2/22 samples positive for coronavirus. Initial genotyping PCR for determination of G- and P-genotype identified 3 samples as G6, 5 samples as P[1] and 1 sample as P[11]. Successful sequencing confirmed that the 3 samples were of genotype G6 but identified the, by genotyping PCR determined, P[1] samples as P[5]. From the coronavirus positive samples one sequence which clustered within the genus betacoronaviruses were retrieved. |
| format | H2 |
| id | RepoSLU5230 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU52302013-01-28T15:40:13Z Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil Diagnostik och molekylär epidemiologi av bovint rotavirus och coronavirus i Brasilien Jakobsson, Samuel molecular epidemiology rotavirus coronavirus calf diarrhea Brazil Rotavirus and coronavirus are the two most common viral causes of neonatal calf diarrhea and their presence causes a lot of economic damage to the farmers as well as suffering to the animal. By getting better knowledge about the viruses we may help in tracing transmission and in producing new vaccines. This paper served to study the molecular epidemiology of rotavirus and coronavirus in fecal samples collected from two different farms in the state of São Paulo, Brazil. These samples have been studied with focus on the gene VP4, VP7, NSP4 in rotavirus and the gene S1 in coronavirus. From the first farm the screening PCR showed that 12/48 samples were positive for rotavirus and 4/48 positive for coronavirus. No determination of genotypes of rotavirus was achieved and the sequences retrieved did not match any known rotavirus. Nor was any amplification and sequencing of the positive samples of coronavirus successful. From the second farm 14/22 samples tested positive for rotavirus and 2/22 samples positive for coronavirus. Initial genotyping PCR for determination of G- and P-genotype identified 3 samples as G6, 5 samples as P[1] and 1 sample as P[11]. Successful sequencing confirmed that the 3 samples were of genotype G6 but identified the, by genotyping PCR determined, P[1] samples as P[5]. From the coronavirus positive samples one sequence which clustered within the genus betacoronaviruses were retrieved. Rotavirus och coronavirus är de två vanligaste virala orsakerna till neonatal diarré hos kalvar och orsakar stora ekonomiska skador för djurägare samt ett lidande för djuren. Genom att få mer kunskap om hur dessa virus är uppbyggda på RNA-nivå kan man hjälpa i smittspårning och i utveckling av nya vacciner. Detta är en studie på molekylär epidemiologi hos dessa virus i fekala prover insamlade från två olika gårdar i delstaten São Paulo, Brasilien. Dessa prover har undersökts med fokus på generna VP4, VP7, NSP4 hos rotavirus samt genen S1 hos coronavirus. I proverna från första gården var 14/48 prover positiva för rotavirus och 4/48 positiva för coronavirus. Genotypning av rotavirus lyckades inte i denna uppsättning och de sekvenser som erhölls liknade inte några andra kända sekvenser av rotavirus. Amplifiering och sekvensering av coronavirus i denna uppsättning lyckades inte. Från den andra gården var 14/22 prover positiva för rotavirus och 2/22 positiva för coronavirus. I genotypande PCR för G- och P-genotyp identifierades 3 prover som G6, 5 prover som P[1] och 1 prov som P[11]. Sekvensering av dessa prover konfirmerade genotypningen som G6 men visade att de tidigare genotypade P[1] istället var av genotyp P[5]. Sekvensering av genen S1 i ett av de två positiva proverna av coronavirus lyckades och denna sekvens placerades i genus betacoronavirus vid en fylogenetisk analys. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2013 H2 eng swe https://stud.epsilon.slu.se/5230/ |
| spellingShingle | molecular epidemiology rotavirus coronavirus calf diarrhea Brazil Jakobsson, Samuel Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title | Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title_full | Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title_fullStr | Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title_full_unstemmed | Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title_short | Diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in Brazil |
| title_sort | diagnosis and molecular epidemiology of bovine rotavirus and coronarvirus in brazil |
| topic | molecular epidemiology rotavirus coronavirus calf diarrhea Brazil |