Identification of protein-interaction partners of ZBED6
ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived fr...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2012
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| Acceso en línea: | https://stud.epsilon.slu.se/5052/ |
| _version_ | 1855570781490118656 |
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| author | Zhou, Mi |
| author_browse | Zhou, Mi |
| author_facet | Zhou, Mi |
| author_sort | Zhou, Mi |
| collection | Epsilon Archive for Student Projects |
| description | ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived from domesticated DNA transposon. However, the mechanism of ZBED6-mediated transcriptional regulation is still unknown. The study of the protein interacting partners of ZBED6 is a straightforward way to help us answer the question. By using the cells with stable overexpression of ZBED6, we perform one-step pull down assay and tandem affinity purification. A protein complex, SFPQ-Nono, is identified as the protein-interaction partners of ZBED6 by using mass spectrometry. |
| format | Second cycle, A2E |
| id | RepoSLU5052 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2012 |
| publishDateSort | 2012 |
| record_format | eprints |
| spelling | RepoSLU50522012-11-23T15:12:38Z https://stud.epsilon.slu.se/5052/ Identification of protein-interaction partners of ZBED6 Zhou, Mi Animal genetics and breeding ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived from domesticated DNA transposon. However, the mechanism of ZBED6-mediated transcriptional regulation is still unknown. The study of the protein interacting partners of ZBED6 is a straightforward way to help us answer the question. By using the cells with stable overexpression of ZBED6, we perform one-step pull down assay and tandem affinity purification. A protein complex, SFPQ-Nono, is identified as the protein-interaction partners of ZBED6 by using mass spectrometry. 2012-11-09 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/5052/13/zhou_m_121123.pdf Zhou, Mi, 2012. Identification of protein-interaction partners of ZBED6. Second cycle, A2E. Uppsala: (VH) > Dept. of Animal Breeding and Genetics (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-670.html> urn:nbn:se:slu:epsilon-s-1871 eng |
| spellingShingle | Animal genetics and breeding Zhou, Mi Identification of protein-interaction partners of ZBED6 |
| title | Identification of protein-interaction partners of ZBED6 |
| title_full | Identification of protein-interaction partners of ZBED6 |
| title_fullStr | Identification of protein-interaction partners of ZBED6 |
| title_full_unstemmed | Identification of protein-interaction partners of ZBED6 |
| title_short | Identification of protein-interaction partners of ZBED6 |
| title_sort | identification of protein-interaction partners of zbed6 |
| topic | Animal genetics and breeding |
| url | https://stud.epsilon.slu.se/5052/ https://stud.epsilon.slu.se/5052/ |