PLA som metod för detektion av bornavirusinfektion hos katt
A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein,...
| Autor principal: | |
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| Formato: | H3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2009
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| Materias: |
| _version_ | 1855570196268318720 |
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| author | Lundqvist, Urban |
| author_browse | Lundqvist, Urban |
| author_facet | Lundqvist, Urban |
| author_sort | Lundqvist, Urban |
| collection | Epsilon Archive for Student Projects |
| description | A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein, as a method for detection of the minute quantities of antigen and antibodies that are present in the tissues and body fluids of a bornaviraly infected cat. For this purpose reliable comparative assays, in the form of an ELISA and an IFA, had to be established. When this proved impossible within the time frame of the project, the aim of the study had to be abandoned, further underscoring the need for improved means of detec-tion of bornaviral antibodies and antigen. |
| format | H3 |
| id | RepoSLU494 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU4942012-04-20T14:09:51Z PLA som metod för detektion av bornavirusinfektion hos katt Lundqvist, Urban Bornavirus Katt Vingelsjuka Diagnostik PLA A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein, as a method for detection of the minute quantities of antigen and antibodies that are present in the tissues and body fluids of a bornaviraly infected cat. For this purpose reliable comparative assays, in the form of an ELISA and an IFA, had to be established. When this proved impossible within the time frame of the project, the aim of the study had to be abandoned, further underscoring the need for improved means of detec-tion of bornaviral antibodies and antigen. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2009 H3 swe eng https://stud.epsilon.slu.se/494/ |
| spellingShingle | Bornavirus Katt Vingelsjuka Diagnostik PLA Lundqvist, Urban PLA som metod för detektion av bornavirusinfektion hos katt |
| title | PLA som metod för detektion av bornavirusinfektion hos katt |
| title_full | PLA som metod för detektion av bornavirusinfektion hos katt |
| title_fullStr | PLA som metod för detektion av bornavirusinfektion hos katt |
| title_full_unstemmed | PLA som metod för detektion av bornavirusinfektion hos katt |
| title_short | PLA som metod för detektion av bornavirusinfektion hos katt |
| title_sort | pla som metod för detektion av bornavirusinfektion hos katt |
| topic | Bornavirus Katt Vingelsjuka Diagnostik PLA |