Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis
Streptococcus agalactiae is an obligate parasite and contagious pathogen living within the epithelial cells lining of acini of the bovine udder, causing milk disease mastitis particularly subclinical mastitis in dairy cattle. This bacterial pathogen also causes infection in pre-milking heifers, as w...
| Autor principal: | |
|---|---|
| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2012
|
| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/4874/ |
| _version_ | 1855570755410984960 |
|---|---|
| author | Zubair, Saima |
| author_browse | Zubair, Saima |
| author_facet | Zubair, Saima |
| author_sort | Zubair, Saima |
| collection | Epsilon Archive for Student Projects |
| description | Streptococcus agalactiae is an obligate parasite and contagious pathogen living within the epithelial cells lining of acini of the bovine udder, causing milk disease mastitis particularly subclinical mastitis in dairy cattle. This bacterial pathogen also causes infection in pre-milking heifers, as well as neonates. We performed whole genome sequencing of strain 09mas018883 of Streptococcus agalactiae. We carried out a reference guided assembly of short paired end reads generated from Illumina Genome Analyzer II using A909 S. agalactiae strain as a reference; as a result a draft genome sequence was generarted. The length of this draft genome sequence is 2127931 bps, consisting of 2129 protein coding genes. We made a comparison of the nickel operons nikABCDE of this genome with those of few others strains of Streptococcus agalactiae to observe the genetic diversification of this region among different strains. Along with pathogenicity islands, these nickel operons might be involved in the conversion of non-pathogenic bacteria to pathogenic through horizontal gene transfer (HGT). |
| format | Second cycle, A2E |
| id | RepoSLU4874 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2012 |
| publishDateSort | 2012 |
| record_format | eprints |
| spelling | RepoSLU48742013-07-16T23:15:13Z https://stud.epsilon.slu.se/4874/ Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis Zubair, Saima Animal genetics and breeding Animal diseases Streptococcus agalactiae is an obligate parasite and contagious pathogen living within the epithelial cells lining of acini of the bovine udder, causing milk disease mastitis particularly subclinical mastitis in dairy cattle. This bacterial pathogen also causes infection in pre-milking heifers, as well as neonates. We performed whole genome sequencing of strain 09mas018883 of Streptococcus agalactiae. We carried out a reference guided assembly of short paired end reads generated from Illumina Genome Analyzer II using A909 S. agalactiae strain as a reference; as a result a draft genome sequence was generarted. The length of this draft genome sequence is 2127931 bps, consisting of 2129 protein coding genes. We made a comparison of the nickel operons nikABCDE of this genome with those of few others strains of Streptococcus agalactiae to observe the genetic diversification of this region among different strains. Along with pathogenicity islands, these nickel operons might be involved in the conversion of non-pathogenic bacteria to pathogenic through horizontal gene transfer (HGT). 2012-10-04 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/4874/7/zubair_z_121004.pdf Zubair, Saima , 2010. Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis. Second cycle, A2E. Uppsala: (VH) > Dept. of Animal Breeding and Genetics (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-670.html> urn:nbn:se:slu:epsilon-s-1720 eng |
| spellingShingle | Animal genetics and breeding Animal diseases Zubair, Saima Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title | Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title_full | Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title_fullStr | Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title_full_unstemmed | Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title_short | Whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| title_sort | whole genome assembly, annotation and bioinformatics analysis of streptococcus agalactiae isolated from cow suffering of mastitis |
| topic | Animal genetics and breeding Animal diseases |
| url | https://stud.epsilon.slu.se/4874/ https://stud.epsilon.slu.se/4874/ |