Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"

Bacillus amyloliquefaciens strain UCMB5113 is a Gram negative rhizo-bacterium, that produces antimicrobial compounds and induce plant basal defense with unknown genetic mechanism that suppress soil-borne plants and pathogens. Paired-end reads of length 75 bp with insert size of ~230 bp were sequence...

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Autor principal: Niazi, Adnan
Formato: H2
Lenguaje:Inglés
Publicado: SLU/Dept. of Animal Breeding and Genetics (until 231231) 2010
Materias:
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author Niazi, Adnan
author_browse Niazi, Adnan
author_facet Niazi, Adnan
author_sort Niazi, Adnan
collection Epsilon Archive for Student Projects
description Bacillus amyloliquefaciens strain UCMB5113 is a Gram negative rhizo-bacterium, that produces antimicrobial compounds and induce plant basal defense with unknown genetic mechanism that suppress soil-borne plants and pathogens. Paired-end reads of length 75 bp with insert size of ~230 bp were sequenced from the genome of UCMB5113 by Illumina multiplex technology. Reference-guided assembly of the paired-end reads was performed using Bacillus amyloliquefaciens FZB42 as reference sequence which was found to be closely related to UCMB5113 strain. A draft of UCMB5113 genome obtained after mapping the reads with reference sequence containing 4,169 genes excluding 90 tRNA and 29 rRNA genes. The draft genome of UCMB5113 harbors cluster of genes, which were found in B. amyloliquefaciens FZB42 and involved in synthesis of secondary metabolites like surfactin, fengycin, bacillomycinD, bacillibactin, and bacilysin, by pathways not involving ribosomes. Initial analysis reveals that UCMB5113 genome lacks an operon NRS involved in synthesis of putative secondary metabolite. The determination of draft genome sequence of UCMB5113 provided very basic analysis of set of genes involved in plant bacterium interaction and plant growth and protection. Further analysis is required to understand the genetic mechanism of interaction and protection mediated by CMB5113.
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id RepoSLU4854
institution Swedish University of Agricultural Sciences
language Inglés
publishDate 2010
publishDateSort 2010
publisher SLU/Dept. of Animal Breeding and Genetics (until 231231)
publisherStr SLU/Dept. of Animal Breeding and Genetics (until 231231)
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spelling RepoSLU48542013-07-16T23:15:12Z Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113" Niazi, Adnan genome assembly Bacillus amyloliquefaciens UCMB5113 plant growth and protection bacterial biocontrol antimicrobial genes Bacillus amyloliquefaciens strain UCMB5113 is a Gram negative rhizo-bacterium, that produces antimicrobial compounds and induce plant basal defense with unknown genetic mechanism that suppress soil-borne plants and pathogens. Paired-end reads of length 75 bp with insert size of ~230 bp were sequenced from the genome of UCMB5113 by Illumina multiplex technology. Reference-guided assembly of the paired-end reads was performed using Bacillus amyloliquefaciens FZB42 as reference sequence which was found to be closely related to UCMB5113 strain. A draft of UCMB5113 genome obtained after mapping the reads with reference sequence containing 4,169 genes excluding 90 tRNA and 29 rRNA genes. The draft genome of UCMB5113 harbors cluster of genes, which were found in B. amyloliquefaciens FZB42 and involved in synthesis of secondary metabolites like surfactin, fengycin, bacillomycinD, bacillibactin, and bacilysin, by pathways not involving ribosomes. Initial analysis reveals that UCMB5113 genome lacks an operon NRS involved in synthesis of putative secondary metabolite. The determination of draft genome sequence of UCMB5113 provided very basic analysis of set of genes involved in plant bacterium interaction and plant growth and protection. Further analysis is required to understand the genetic mechanism of interaction and protection mediated by CMB5113. SLU/Dept. of Animal Breeding and Genetics (until 231231) 2010 H2 eng https://stud.epsilon.slu.se/4854/
spellingShingle genome assembly
Bacillus amyloliquefaciens UCMB5113
plant growth and protection
bacterial biocontrol
antimicrobial genes
Niazi, Adnan
Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title_full Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title_fullStr Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title_full_unstemmed Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title_short Whole genome assembly, annotation and bioinformatics analysis of Bacillus amyloliquefaciens strain UCMB5113"
title_sort whole genome assembly, annotation and bioinformatics analysis of bacillus amyloliquefaciens strain ucmb5113"
topic genome assembly
Bacillus amyloliquefaciens UCMB5113
plant growth and protection
bacterial biocontrol
antimicrobial genes