Patho-anatomical studies on african swine fever in Uganda
African Swine Fever (ASF) is one of the most serious transboundary swine diseases because of its high lethality for pigs, socioeconomic consequences, rapid and international spread and the absence of either treatment or vaccine. This Sida Minor Field Study (MFS) was carried out during fall 2010, as...
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| Formato: | L3 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2012
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| Materias: |
| _version_ | 1855570705989500928 |
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| author | Ganowiak, Justine |
| author_browse | Ganowiak, Justine |
| author_facet | Ganowiak, Justine |
| author_sort | Ganowiak, Justine |
| collection | Epsilon Archive for Student Projects |
| description | African Swine Fever (ASF) is one of the most serious transboundary swine diseases because of its high lethality for pigs, socioeconomic consequences, rapid and international spread and the absence of either treatment or vaccine. This Sida Minor Field Study (MFS) was carried out during fall 2010, as part of a larger collaborative research project called ASFUganda, focusing on the epidemiology of the disease in Uganda. The aim with this study was to get more knowledge about the pathology in cases of ASF in Uganda, by studying macroscopic and microscopic lesions in pigs with acute and chronic ASF and to detect the ASF virus (ASFV) in tissues by immunohistochemistry.
The pigs were selected from the two different geographical locations in Uganda, Mityana and Gulu district, both with on going confirmed outbreaks of ASF. Necropsies were performed in the field, and the laboratory procedures at the JICA (Japan International Cooperation Agency) Veterinary Pathology Laboratory, Entebbe, Uganda, and at the Department of BVF, SLU, Uppsala, Sweden. Three pigs from an outbreak of ASF in Mityana district showed both the history and the clinical symptoms typical for ASF. The macroscopic and histopathological findings were consistent with the lesions described in the literature for acute ASF. ASFV antigen was detected by immunohistochemistry in the lymphoid tissues from these pigs, indicating that the lesions were the direct result of the virus infection. Three pigs from Gulu district did not show any clinical symptoms of ASF disease before slaughter but were positive for ASFV on RT-PCR and ELISA. The macroscopic and histopathological findings resembled the lesions previously described for subacute and chronic ASF. |
| format | L3 |
| id | RepoSLU4585 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU45852012-10-04T08:14:09Z Patho-anatomical studies on african swine fever in Uganda Patologanatomiska studier av afrikansk svinpest i Uganda Ganowiak, Justine African Swine Fever pathology Uganda African Swine Fever (ASF) is one of the most serious transboundary swine diseases because of its high lethality for pigs, socioeconomic consequences, rapid and international spread and the absence of either treatment or vaccine. This Sida Minor Field Study (MFS) was carried out during fall 2010, as part of a larger collaborative research project called ASFUganda, focusing on the epidemiology of the disease in Uganda. The aim with this study was to get more knowledge about the pathology in cases of ASF in Uganda, by studying macroscopic and microscopic lesions in pigs with acute and chronic ASF and to detect the ASF virus (ASFV) in tissues by immunohistochemistry. The pigs were selected from the two different geographical locations in Uganda, Mityana and Gulu district, both with on going confirmed outbreaks of ASF. Necropsies were performed in the field, and the laboratory procedures at the JICA (Japan International Cooperation Agency) Veterinary Pathology Laboratory, Entebbe, Uganda, and at the Department of BVF, SLU, Uppsala, Sweden. Three pigs from an outbreak of ASF in Mityana district showed both the history and the clinical symptoms typical for ASF. The macroscopic and histopathological findings were consistent with the lesions described in the literature for acute ASF. ASFV antigen was detected by immunohistochemistry in the lymphoid tissues from these pigs, indicating that the lesions were the direct result of the virus infection. Three pigs from Gulu district did not show any clinical symptoms of ASF disease before slaughter but were positive for ASFV on RT-PCR and ELISA. The macroscopic and histopathological findings resembled the lesions previously described for subacute and chronic ASF. Afrikansk svinpest (ASF) är en av de mest allvarliga sjukdomarna hos svin på grund av dess höga dödlighet, socioekonomiska konsekvenser, snabba och internationella spridning samt frånvaron av varken behandling eller vaccin. Det här Sida-finanserade MFS-arbetet (MFS=Minor Field Study) utfördes hösten 2010 som en del av ett större samarbetsprojekt kallat ASFUganda, med fokus på sjukdomens epidemiologi i Uganda. Syftet med studien var att få ökad kunskap om patologiska förändringar vid fall av ASF, genom att undersöka makroskopiska och mikroskopiska förändringar hos svin med akut och kronisk ASF samt att påvisa ASF viruset (ASFV) i vävnader med hjälp av immunohistokemi. Grisarna i studien var utvalda från två olika geografiska platser i Uganda, distriktet Mityana och distriktet Gulu, båda med verifierade pågående utbrott av ASF. Obduktioner utfördes i fält och laboratoriearbetet på JICAs (Japan International Cooperation Agency) Veterinary Pathology Laboratory i Entebbe, Uganda, samt på institutionen för biomedicin och veterinär folkhälsovetenskap (BVF), SLU, Uppsala, Sverige. Tre grisar från ett pågående utbrott av ASF i distriktet Mityana (oktober-december 2010) visade både anamnes och kliniska symptom typiska för akut ASF. De makroskopiska och histopatologiska fynden överensstämde med de förändringar beskrivna i litteraturen för akut ASF. Påvisandet av ASFV via immunohistokemi i den lymfatiska vävnaden från dessa grisar indikerar att lesionerna orsakats av virusinfektionen. Tre grisar från distriktet Gulu visade inte några kliniska symptom på ASF före slakt men var RT-PCR-positiva och ELISA-positiva för ASFV. De makroskopiska och histopatologiska fynden liknade de förändringar beskrivna i litteraturen för subakut och kronisk ASF. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2012 L3 eng swe https://stud.epsilon.slu.se/4585/ |
| spellingShingle | African Swine Fever pathology Uganda Ganowiak, Justine Patho-anatomical studies on african swine fever in Uganda |
| title | Patho-anatomical studies on african swine fever in Uganda |
| title_full | Patho-anatomical studies on african swine fever in Uganda |
| title_fullStr | Patho-anatomical studies on african swine fever in Uganda |
| title_full_unstemmed | Patho-anatomical studies on african swine fever in Uganda |
| title_short | Patho-anatomical studies on african swine fever in Uganda |
| title_sort | patho-anatomical studies on african swine fever in uganda |
| topic | African Swine Fever pathology Uganda |