Infectious bronchitis in Brazil
Infectious Bronchitis (IB) is a highly contagious viral respiratory and production depressant disease distributed world wide. The disease is caused by the Infectious Bronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within the genus Gammacoronavirus. Due to a combination of high mutati...
| Autor principal: | |
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| Formato: | Second cycle, A1N, A1F or AXX |
| Lenguaje: | sueco Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/3940/ |
| _version_ | 1855570613294333952 |
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| author | Olofsson, Cecilia |
| author_browse | Olofsson, Cecilia |
| author_facet | Olofsson, Cecilia |
| author_sort | Olofsson, Cecilia |
| collection | Epsilon Archive for Student Projects |
| description | Infectious Bronchitis (IB) is a highly contagious viral respiratory and production
depressant disease distributed world wide. The disease is caused by the Infectious
Bronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within the
genus Gammacoronavirus. Due to a combination of high mutation rate and a
tendency of recombination a constant emergence of new genotypes and serotypes
is seen. In Brazil, a country with an extensive poultry production and major
problems with disease control, the diversity of circulating strains are wide and
outbreaks of clinical disease despite intensive use of vaccines are common. The
aim of this study was to investigate the presence of IBV and to identify genotypes
of isolated IBVs in a limited number of layer flocks in Bastos, São Paulo, as part
of the screening for circulating genotypes in the country. As a minor part the
Biosecurity measures (precautions) was observed. In addition, a literature study
was performed on IB and IBV. Organ samples from four farms were collected as
pools of trachea, lungs, kidneys, reproductive tract and enteric content and were
analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).
Over fifty percent of the samples were positive for IBV and three samples were
identified as 4/91-genotype using Genotype-specific PCR. Due to problems with
the RT-PCR that was targeting part of the S1-gene, the genotypes of the rest of the
positive samples could not be determined using DNA sequencing. They were
instead identified as not belonging to Massachusetts, D274 or 4/91 genotypes
using Genotype-specific RT-PCR. Considering the results of previous studies in
Brazil stating that a national Brazilian Variant dominates, the possibility that the
unidentified genotypes belonged to the national genotype were considered high. |
| format | Second cycle, A1N, A1F or AXX |
| id | RepoSLU3940 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2012 |
| publishDateSort | 2012 |
| record_format | eprints |
| spelling | RepoSLU39402012-04-20T14:25:29Z https://stud.epsilon.slu.se/3940/ Infectious bronchitis in Brazil Olofsson, Cecilia Human medicine, health, and safety Infectious Bronchitis (IB) is a highly contagious viral respiratory and production depressant disease distributed world wide. The disease is caused by the Infectious Bronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within the genus Gammacoronavirus. Due to a combination of high mutation rate and a tendency of recombination a constant emergence of new genotypes and serotypes is seen. In Brazil, a country with an extensive poultry production and major problems with disease control, the diversity of circulating strains are wide and outbreaks of clinical disease despite intensive use of vaccines are common. The aim of this study was to investigate the presence of IBV and to identify genotypes of isolated IBVs in a limited number of layer flocks in Bastos, São Paulo, as part of the screening for circulating genotypes in the country. As a minor part the Biosecurity measures (precautions) was observed. In addition, a literature study was performed on IB and IBV. Organ samples from four farms were collected as pools of trachea, lungs, kidneys, reproductive tract and enteric content and were analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Over fifty percent of the samples were positive for IBV and three samples were identified as 4/91-genotype using Genotype-specific PCR. Due to problems with the RT-PCR that was targeting part of the S1-gene, the genotypes of the rest of the positive samples could not be determined using DNA sequencing. They were instead identified as not belonging to Massachusetts, D274 or 4/91 genotypes using Genotype-specific RT-PCR. Considering the results of previous studies in Brazil stating that a national Brazilian Variant dominates, the possibility that the unidentified genotypes belonged to the national genotype were considered high. 2012-03-05 Second cycle, A1N, A1F or AXX NonPeerReviewed application/pdf swe https://stud.epsilon.slu.se/3940/7/olofsson_c_%202012.pdf Olofsson, Cecilia, 2012. Infectious bronchitis in Brazil : a minor field study in Bastos municipality, Sao Paulo. Second cycle, A1N, A1F or AXX ( AXX). Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-984 eng |
| spellingShingle | Human medicine, health, and safety Olofsson, Cecilia Infectious bronchitis in Brazil |
| title | Infectious bronchitis in Brazil |
| title_full | Infectious bronchitis in Brazil |
| title_fullStr | Infectious bronchitis in Brazil |
| title_full_unstemmed | Infectious bronchitis in Brazil |
| title_short | Infectious bronchitis in Brazil |
| title_sort | infectious bronchitis in brazil |
| topic | Human medicine, health, and safety |
| url | https://stud.epsilon.slu.se/3940/ https://stud.epsilon.slu.se/3940/ |