The significance of oxytocin in canine mammary tumours
Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissue...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2012
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| Acceso en línea: | https://stud.epsilon.slu.se/3932/ |
| _version_ | 1855570611720421376 |
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| author | Bergman, Ingrid |
| author_browse | Bergman, Ingrid |
| author_facet | Bergman, Ingrid |
| author_sort | Bergman, Ingrid |
| collection | Epsilon Archive for Student Projects |
| description | Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane.
The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells. Immunocytochemistry was used to detect possible oxytocin receptors.
The results showed that there was significant inhibition of the cell proliferation with the addition of oxytocin. In three different assays at least one cell concentration showed significant inhibition of the cell growth. The immunocytochemistry analysis showed the presence of oxytocin receptors and based on the location of the receptors it seemed to be at least two groups within the cell line. Some of the cells show marked staining in the nuclear membrane and some seemed to be unstained or had weak staining in the cell membrane or cytoplasm.
Since the location of the oxytocin receptors show that they have been activated, further studies can find out which of the cells that produce oxytocin, and if the possible synthesis leads to paracrine or autocrine stimulation.
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| format | Second cycle, A2E |
| id | RepoSLU3932 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2012 |
| publishDateSort | 2012 |
| record_format | eprints |
| spelling | RepoSLU39322012-04-23T07:46:49Z https://stud.epsilon.slu.se/3932/ The significance of oxytocin in canine mammary tumours Bergman, Ingrid Animal diseases Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane. The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells. Immunocytochemistry was used to detect possible oxytocin receptors. The results showed that there was significant inhibition of the cell proliferation with the addition of oxytocin. In three different assays at least one cell concentration showed significant inhibition of the cell growth. The immunocytochemistry analysis showed the presence of oxytocin receptors and based on the location of the receptors it seemed to be at least two groups within the cell line. Some of the cells show marked staining in the nuclear membrane and some seemed to be unstained or had weak staining in the cell membrane or cytoplasm. Since the location of the oxytocin receptors show that they have been activated, further studies can find out which of the cells that produce oxytocin, and if the possible synthesis leads to paracrine or autocrine stimulation. Oxytocin är ett hormon som spelar en viktig roll i många funktioner för reproduktion och beteende. Det påverkar många organ och vävnader, de klassiska perifera målorganen är mjölkkörtlarna vid laktation och livmodern under förlossning. Oxytocinreceptorer har nyligen beskrivits i en mängd normala vävnader och primära cellkulturer, men även i neoplastisk vävnad och etablerade neoplastiska cellinjer, som cellinjer från bröstcancertumörer. Signalvägarna och de biologiska effekterna av oxytocin/oxytocinreceptor-systemet verkar bero på art, vävnadstyp, fysiologisk kontra neoplastisk status och receptorns plats i cellmembranet. Syftet med det här projektet var att ta reda på om celltillväxten i cellinjen CMT-U27 från juvercarcinom hos hund påverkades av oxytocinstimulering samt att undersöka förekomsten av oxytocinreceptorer i dessa celler. Celltillväxten analyserades med hjälp av ett ELISA-kit, där den uppmätta absorbansen är relativ mot antalet levande celler. Immunocytokemi användes för att undersöka förekomsten av oxytocinreceptorer. Resultaten visade på signifikant inhibering av celltillväxten vid tillsats av oxytocin. I tre olika analysomgångar visade minst en cellkoncentration på signifikant inhibering av celltillväxten. Immunocytokemianalysen påvisade förekomst av oxytocinreceptorer och baserat på receptorernas placering verkade det finnas minst två grupper inom cellinjen. Några celler var starkt infärgade i kärnmembranet och några verkade helt ofärgade eller hade svagare infärgning i cellmembranet eller cytoplasman. Eftersom oxytocinreceptorernas position visar att de blivit aktiverade kan ytterligare studier ta reda på vilka celler som producerar oxytocin, samt om det leder till parakin eller autokrin stimulering. 2012-02-27 Second cycle, A2E NonPeerReviewed application/pdf swe https://stud.epsilon.slu.se/3932/1/Bergman_I_120216.pdf Bergman, Ingrid, 2012. The significance of oxytocin in canine mammary tumours. Second cycle, A2E. Uppsala: (VH) > Dept. of Anatomy, Physiology and Biochemistry (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-712.html> urn:nbn:se:slu:epsilon-s-962 eng |
| spellingShingle | Animal diseases Bergman, Ingrid The significance of oxytocin in canine mammary tumours |
| title | The significance of oxytocin in canine mammary tumours |
| title_full | The significance of oxytocin in canine mammary tumours |
| title_fullStr | The significance of oxytocin in canine mammary tumours |
| title_full_unstemmed | The significance of oxytocin in canine mammary tumours |
| title_short | The significance of oxytocin in canine mammary tumours |
| title_sort | significance of oxytocin in canine mammary tumours |
| topic | Animal diseases |
| url | https://stud.epsilon.slu.se/3932/ https://stud.epsilon.slu.se/3932/ |