Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora

Production of ethanol using cellulosic material as feedstock is crucial for sustainable fuel ethanol production.However a production process based on cellulosic biomass involves several energy and cost intensive steps like storage of biomass, pretreatment, hydrolysis and fermentation, where pretreat...

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Autor principal: Anjaly Sukumaran Nair, Harikrishnan
Formato: H2
Lenguaje:Inglés
Publicado: SLU/Dept. of Microbiology 2011
Materias:
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author Anjaly Sukumaran Nair, Harikrishnan
author_browse Anjaly Sukumaran Nair, Harikrishnan
author_facet Anjaly Sukumaran Nair, Harikrishnan
author_sort Anjaly Sukumaran Nair, Harikrishnan
collection Epsilon Archive for Student Projects
description Production of ethanol using cellulosic material as feedstock is crucial for sustainable fuel ethanol production.However a production process based on cellulosic biomass involves several energy and cost intensive steps like storage of biomass, pretreatment, hydrolysis and fermentation, where pretreatment is the energy intensive and troublesome step. This project aimed for an integration of storage and pretreatment step, to get more energy efficiency and more ethanol yield. In the present investigation wheat strawwas used as a model and was stored in moist conditions with different fungal species (Pichia anomala, Pichia stipitis, and Anthracophyllum discolor) inoculated separately in mini-silos for 1 month at 15°C and 4°C. Simultaneous saccharification and fermentation was carried out afterthe storage period and ethanol yields were compared with dry wheat straw as a control.A7.52 % higher ethanol yield (compared to the dry wheat straw) was obtained from wheat straw incubated by P. anomala at 15°C, and 6.87 % higher ethanol yield from P.stipitis inoculated wheat straw incubated at 4°C showed ISP can result in increasing the ethanol yield. Also it was obvious from the study that, the release of sugar from integrated storage and pretreatment (ISP) sample was faster than from the traditional sample. The higher concentration of non-fermentable sugars (eg: xylose, arabinose, mannose etc.) left during fermentation of ISP samples indicate that the ISP process causes more structural damage to the cellulosic substances and produces more sugar release than the control. Moreover P. anomala and P.stipitis showed a biocontrol activity during moist storageby preventing growth of other fungi and enterobacteria in the wheat straw during the one month incubation. In conclusion, ISP acted as an efficient method of storage and resulted in higher ethanol yield.
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institution Swedish University of Agricultural Sciences
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spelling RepoSLU37922012-04-20T14:24:37Z Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora Anjaly Sukumaran Nair, Harikrishnan Biofuel wet storage Biocontrol Dilute acid pretreatment Simultaneous Pichia anomala Pichia stipitis Anthracophyllum discolor Production of ethanol using cellulosic material as feedstock is crucial for sustainable fuel ethanol production.However a production process based on cellulosic biomass involves several energy and cost intensive steps like storage of biomass, pretreatment, hydrolysis and fermentation, where pretreatment is the energy intensive and troublesome step. This project aimed for an integration of storage and pretreatment step, to get more energy efficiency and more ethanol yield. In the present investigation wheat strawwas used as a model and was stored in moist conditions with different fungal species (Pichia anomala, Pichia stipitis, and Anthracophyllum discolor) inoculated separately in mini-silos for 1 month at 15°C and 4°C. Simultaneous saccharification and fermentation was carried out afterthe storage period and ethanol yields were compared with dry wheat straw as a control.A7.52 % higher ethanol yield (compared to the dry wheat straw) was obtained from wheat straw incubated by P. anomala at 15°C, and 6.87 % higher ethanol yield from P.stipitis inoculated wheat straw incubated at 4°C showed ISP can result in increasing the ethanol yield. Also it was obvious from the study that, the release of sugar from integrated storage and pretreatment (ISP) sample was faster than from the traditional sample. The higher concentration of non-fermentable sugars (eg: xylose, arabinose, mannose etc.) left during fermentation of ISP samples indicate that the ISP process causes more structural damage to the cellulosic substances and produces more sugar release than the control. Moreover P. anomala and P.stipitis showed a biocontrol activity during moist storageby preventing growth of other fungi and enterobacteria in the wheat straw during the one month incubation. In conclusion, ISP acted as an efficient method of storage and resulted in higher ethanol yield. SLU/Dept. of Microbiology 2011 H2 eng https://stud.epsilon.slu.se/3792/
spellingShingle Biofuel
wet storage
Biocontrol
Dilute acid pretreatment
Simultaneous
Pichia anomala
Pichia stipitis
Anthracophyllum discolor
Anjaly Sukumaran Nair, Harikrishnan
Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title_full Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title_fullStr Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title_full_unstemmed Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title_short Integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
title_sort integrated storage and pretreatment of wheat straw with different fungi : impact on ethanol production and storage microflora
topic Biofuel
wet storage
Biocontrol
Dilute acid pretreatment
Simultaneous
Pichia anomala
Pichia stipitis
Anthracophyllum discolor