Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia

Occurrence, characterization, detection of enterotoxin and antimicrobial susceptibility profile in staphylococci isolated from yak and cattle in Mongolia were investigated. Staphylococci were isolated from 72 (74%) of the 97 raw milk samples investigated. Of the samples containing staphylococci, 69%...

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Autor principal: Tsegmed, Uranchimeg
Formato: Second cycle, A1N, A1F or AXX
Lenguaje:Inglés
Inglés
Publicado: 2011
Materias:
Acceso en línea:https://stud.epsilon.slu.se/3336/
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author Tsegmed, Uranchimeg
author_browse Tsegmed, Uranchimeg
author_facet Tsegmed, Uranchimeg
author_sort Tsegmed, Uranchimeg
collection Epsilon Archive for Student Projects
description Occurrence, characterization, detection of enterotoxin and antimicrobial susceptibility profile in staphylococci isolated from yak and cattle in Mongolia were investigated. Staphylococci were isolated from 72 (74%) of the 97 raw milk samples investigated. Of the samples containing staphylococci, 69% (50/72) were from yak, whereas 31% (22/72) were from cattle. Of the samples containing staphylococci, S. aureus was detected in 14% (7/50) of yak milk samples and in 68% (15/22) of cattle samples. Staphylococcal enterotoxin C (SEC) was detected in 19% (5/26) of the S. aureus strains investigated. Three of the enterotoxigenic strains were from yak and two from cattle. None of the S. aureus strains tested produced SEA, SEB, or SED. The MICs of 12 antimicrobial agents for 45 isolates of Staphylococcus spp. from yak and cattle were determined. Broth microdilution was used for the susceptibility testing and because of high oxacillin MICs all isolates were also subjected to oxacillin agar screening and PCR for the mecA gene. Nitrocefin test was used to determine β-lactamase production. The proportion of resistance to β-lactamse based on β-lactamase production was high (37-84%). However, no mecA gene was detected. Resistance to erythromycin, clindamycin, chloramphenicol, tetracycline, trimethoprim and fusidic acid was recorded among isolates from both yak and cattle. Cephalothin resistance was found only among coagulase-negative staphylococci from yak.
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Inglés
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spelling RepoSLU33362012-04-20T14:22:50Z https://stud.epsilon.slu.se/3336/ Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia Tsegmed, Uranchimeg Animal diseases Occurrence, characterization, detection of enterotoxin and antimicrobial susceptibility profile in staphylococci isolated from yak and cattle in Mongolia were investigated. Staphylococci were isolated from 72 (74%) of the 97 raw milk samples investigated. Of the samples containing staphylococci, 69% (50/72) were from yak, whereas 31% (22/72) were from cattle. Of the samples containing staphylococci, S. aureus was detected in 14% (7/50) of yak milk samples and in 68% (15/22) of cattle samples. Staphylococcal enterotoxin C (SEC) was detected in 19% (5/26) of the S. aureus strains investigated. Three of the enterotoxigenic strains were from yak and two from cattle. None of the S. aureus strains tested produced SEA, SEB, or SED. The MICs of 12 antimicrobial agents for 45 isolates of Staphylococcus spp. from yak and cattle were determined. Broth microdilution was used for the susceptibility testing and because of high oxacillin MICs all isolates were also subjected to oxacillin agar screening and PCR for the mecA gene. Nitrocefin test was used to determine β-lactamase production. The proportion of resistance to β-lactamse based on β-lactamase production was high (37-84%). However, no mecA gene was detected. Resistance to erythromycin, clindamycin, chloramphenicol, tetracycline, trimethoprim and fusidic acid was recorded among isolates from both yak and cattle. Cephalothin resistance was found only among coagulase-negative staphylococci from yak. 2011-10-26 Second cycle, A1N, A1F or AXX NonPeerReviewed application/pdf eng https://stud.epsilon.slu.se/3336/1/Tsegmed_U_111012.pdf Tsegmed, Uranchimeg, 2006. Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia : studies on the occurrence, characterization, detection of enterotoxin and antimicrobial susceptibility profile of the isolates. Second cycle, A1N, A1F or AXX ( AXX). Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-660 eng
spellingShingle Animal diseases
Tsegmed, Uranchimeg
Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title_full Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title_fullStr Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title_full_unstemmed Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title_short Staphylococci Isolated from Raw Milk of Yak and Cattle in Mongolia
title_sort staphylococci isolated from raw milk of yak and cattle in mongolia
topic Animal diseases
url https://stud.epsilon.slu.se/3336/
https://stud.epsilon.slu.se/3336/