Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger
There are several fungal species including Aspergillus niger that are responsible for food spoilage. The conidia of Aspergillus niger contain mannitol and trehalose and these compounds act as stress protectants and keep the cells viable. In my experiments, I observed the survival after stress in tre...
| Autor principal: | |
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| Formato: | First cycle, G2E |
| Lenguaje: | Inglés Inglés |
| Publicado: |
2011
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| Acceso en línea: | https://stud.epsilon.slu.se/2512/ |
| _version_ | 1855570442786439168 |
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| author | Tajrin, Tania |
| author_browse | Tajrin, Tania |
| author_facet | Tajrin, Tania |
| author_sort | Tajrin, Tania |
| collection | Epsilon Archive for Student Projects |
| description | There are several fungal species including Aspergillus niger that are responsible for food
spoilage. The conidia of Aspergillus niger contain mannitol and trehalose and these
compounds act as stress protectants and keep the cells viable. In my experiments, I
observed the survival after stress in trehalose and mannitol deficient mutants of
Aspergillus niger and compared with wild type strains. I applied different treatments like
heat shock, freeze-drying and different energy sources on mutants and wild types spores
at different ages. The results indicate that 1-month-old spores of mutant of ΔtppB and
ΔmpdA are more vulnerable at high and low temperatures. These experiments indicate
that the trehalose deficient mutant ΔtppB and the mannitol deficient mutant ΔmpdA are
more sensitive compared to wild type and most probably due to their lower mannitol and
trehalose levels. |
| format | First cycle, G2E |
| id | RepoSLU2512 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés Inglés |
| publishDate | 2011 |
| publishDateSort | 2011 |
| record_format | eprints |
| spelling | RepoSLU25122012-04-20T14:18:58Z https://stud.epsilon.slu.se/2512/ Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger Tajrin, Tania Food science and technology Food contamination and toxicology There are several fungal species including Aspergillus niger that are responsible for food spoilage. The conidia of Aspergillus niger contain mannitol and trehalose and these compounds act as stress protectants and keep the cells viable. In my experiments, I observed the survival after stress in trehalose and mannitol deficient mutants of Aspergillus niger and compared with wild type strains. I applied different treatments like heat shock, freeze-drying and different energy sources on mutants and wild types spores at different ages. The results indicate that 1-month-old spores of mutant of ΔtppB and ΔmpdA are more vulnerable at high and low temperatures. These experiments indicate that the trehalose deficient mutant ΔtppB and the mannitol deficient mutant ΔmpdA are more sensitive compared to wild type and most probably due to their lower mannitol and trehalose levels. 2011-04-18 First cycle, G2E NonPeerReviewed application/pdf eng https://stud.epsilon.slu.se/2512/1/Tajrin_t_110418.pdf Tajrin, Tania, 2011. Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger. First cycle, G2E. Uppsala: (NL, NJ) > Dept. of Microbiology <https://stud.epsilon.slu.se/view/divisions/4024.html> urn:nbn:se:slu:epsilon-s-63 eng |
| spellingShingle | Food science and technology Food contamination and toxicology Tajrin, Tania Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger |
| title | Stress tolerance and growth of trehalose
and mannitol deficient mutants in
Aspergillus niger |
| title_full | Stress tolerance and growth of trehalose
and mannitol deficient mutants in
Aspergillus niger |
| title_fullStr | Stress tolerance and growth of trehalose
and mannitol deficient mutants in
Aspergillus niger |
| title_full_unstemmed | Stress tolerance and growth of trehalose
and mannitol deficient mutants in
Aspergillus niger |
| title_short | Stress tolerance and growth of trehalose
and mannitol deficient mutants in
Aspergillus niger |
| title_sort | stress tolerance and growth of trehalose
and mannitol deficient mutants in
aspergillus niger |
| topic | Food science and technology Food contamination and toxicology |
| url | https://stud.epsilon.slu.se/2512/ https://stud.epsilon.slu.se/2512/ |