Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands
Roseroot (R. rosea L.) has gained more attention the last years, mainly because of its phy-topharmacologic properties. Even though many reports in roseroot have been published, only a few articles are dealt with the genetic diversity. The Nordic Genetic Resource Center has collected Roseroot materi...
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| Formato: | H2 |
| Lenguaje: | Inglés sueco |
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SLU/Plant Breeding and Biotechnology (until 121231)
2010
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| Materias: |
| _version_ | 1855570380861734912 |
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| author | Kylin, Madeleine |
| author_browse | Kylin, Madeleine |
| author_facet | Kylin, Madeleine |
| author_sort | Kylin, Madeleine |
| collection | Epsilon Archive for Student Projects |
| description | Roseroot (R. rosea L.) has gained more attention the last years, mainly because of its phy-topharmacologic properties. Even though many reports in roseroot have been published, only a few articles are dealt with the genetic diversity.
The Nordic Genetic Resource Center has collected Roseroot material from Sweden, Greenland and Faroe Islands under different expeditions. The samples were analysed to study the genetic diversity study. Such studies would facilitate The Nordic Genetic Re-source Center (NordGen) in conservation of roseroot in its gene bank.
In this study, the roseroot materials collected in the NGB were analysed using two dif-ferent DNA marker methods, microsatellites (SSR) and inter simple sequence repeats (ISSR) for evaluating the genetic diversity. PCR products were separated on polyacryla-mide gels and developed with fast and sensitive silver staining. Gels were read manually with replications. Co-dominant and dominant marker data were analysed separately using POPGENE 1.32; and NTSYSpc 2.11.
Four ISSR primers and four SSR primers were studied and resulted in 37 and 12 poly-morphic bands, respectively. Percentages of polymorphic bands for ISSR markers were for Sweden 83.78%, Greenland 94.59% and Faroe Islands 48.65%. Only two samples appear to be clonal material, the rest 89 samples are unique. No gender-specific or population-specific primers were found.
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| format | H2 |
| id | RepoSLU2049 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | SLU/Plant Breeding and Biotechnology (until 121231) |
| publisherStr | SLU/Plant Breeding and Biotechnology (until 121231) |
| record_format | eprints |
| spelling | RepoSLU20492012-04-20T14:16:54Z Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands Kylin, Madeleine Rhodiola rosea Roseroot genetic diversity microsatellites inter-simple sequence repeat Roseroot (R. rosea L.) has gained more attention the last years, mainly because of its phy-topharmacologic properties. Even though many reports in roseroot have been published, only a few articles are dealt with the genetic diversity. The Nordic Genetic Resource Center has collected Roseroot material from Sweden, Greenland and Faroe Islands under different expeditions. The samples were analysed to study the genetic diversity study. Such studies would facilitate The Nordic Genetic Re-source Center (NordGen) in conservation of roseroot in its gene bank. In this study, the roseroot materials collected in the NGB were analysed using two dif-ferent DNA marker methods, microsatellites (SSR) and inter simple sequence repeats (ISSR) for evaluating the genetic diversity. PCR products were separated on polyacryla-mide gels and developed with fast and sensitive silver staining. Gels were read manually with replications. Co-dominant and dominant marker data were analysed separately using POPGENE 1.32; and NTSYSpc 2.11. Four ISSR primers and four SSR primers were studied and resulted in 37 and 12 poly-morphic bands, respectively. Percentages of polymorphic bands for ISSR markers were for Sweden 83.78%, Greenland 94.59% and Faroe Islands 48.65%. Only two samples appear to be clonal material, the rest 89 samples are unique. No gender-specific or population-specific primers were found. Rosenrot (R.rosea L.) är en växt som fått mer intresse om sig de senaste åren, mestadels för dess fytofarmakologiska egenskaper. Även om många artiklar om rosenrot har blivit publicerade, så är det endast ett fåtal som handlar om dess genetiska diversitet. Nordiskt Genresurscenter har samlat in material från rosenrot ifrån Sverige, Grönland och Färöarna under olika expeditioner. Proven blev analyserade för att studera den genetiska diversiteten. Resultat från en genetisk diversitetsstudie kan hjälpa Nordiskt Genresurscen-ter i deras bevarandesyfte för sin genbank. I den här studien har insamlat material från NordGen blivit analyserade av två olika DNA markör metoder, mikrosatelliter (SSR) och inter simple sequence repeats (ISSR) för att utvärdera den genetiska diversiten. PCR produkter blev separerade på en polyacryla-midgel och framkallades med en silvermetod. Gelerna blev avlästa manuellt med replika-tioner. Co-dominant och dominant markör data blev separat analyserade med hjälp av POPGENE 1.32; och NTSYSpc 2.11. Fyra ISSR-primers och fyra SSR-primers blev studerade och resulterade i 37 (ISSR) och 12 polymorfiska band (SSR). Procent av polymorfiska band för ISSR markörer var för Sverige 83.78%, Grönland 94.59% och Färöarna 48.65%. Bara två prover visade sig vara klonmaterial, de resterande 89 proverna var unika. Inga könsspecifika eller populations-specifika primers hittades. SLU/Plant Breeding and Biotechnology (until 121231) 2010 H2 eng swe https://stud.epsilon.slu.se/2049/ |
| spellingShingle | Rhodiola rosea Roseroot genetic diversity microsatellites inter-simple sequence repeat Kylin, Madeleine Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title | Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title_full | Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title_fullStr | Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title_full_unstemmed | Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title_short | Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands |
| title_sort | genetic diversity of roseroot (rhodiola rosea l.) from sweden, greenland and faroe islands |
| topic | Rhodiola rosea Roseroot genetic diversity microsatellites inter-simple sequence repeat |