Fungicide resistance against fluopyram in Alternaria solani

Early blight in potato production can be devastating and to minimize crop damage the use of fungicides has been the key. New fungicides called the SDHIs have been used against the early blight pathogen, Alternaria Solani in potato fields to reduce yield losses. However, repeated fungicide applicatio...

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Autor principal: Ekdahl, Felicia
Formato: H2
Lenguaje:Inglés
Publicado: SLU/Dept. of Plant Protection Biology 2022
Materias:
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author Ekdahl, Felicia
author_browse Ekdahl, Felicia
author_facet Ekdahl, Felicia
author_sort Ekdahl, Felicia
collection Epsilon Archive for Student Projects
description Early blight in potato production can be devastating and to minimize crop damage the use of fungicides has been the key. New fungicides called the SDHIs have been used against the early blight pathogen, Alternaria Solani in potato fields to reduce yield losses. However, repeated fungicide applications run the risk of fungicide resistance development in the fungal population, through the selection of mutations in the genes that are targeted by the fungicides. Mutations that confer a loss of sensitivity to the fungicide (i.e., fungicide resistance) will be selected for in fungal populations where there is a significant selection pressure, through for example, the repeated use of one class of fungicides. To investigate whether A. solani was becoming insensitive to the new SDHI fungicide fluopyram, a growth study was conducted where 20 different strains of A.solani were grown on solid medium with five different concentrations to see if some of the strains were less susceptible than others. It was difficult to draw in depth conclusions from this growth study, however some samples showed less sensitivity to the fluopyram. A DNA analysis of the fluopyram target gene was also performed to try to determine if there were any mutations associated with resistance present. However, due to problems with the PCR and sequencing experiments it was not possible to make any firm conclusions from this.
format H2
id RepoSLU17920
institution Swedish University of Agricultural Sciences
language Inglés
publishDate 2022
publishDateSort 2022
publisher SLU/Dept. of Plant Protection Biology
publisherStr SLU/Dept. of Plant Protection Biology
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spelling RepoSLU179202022-07-08T10:32:18Z Fungicide resistance against fluopyram in Alternaria solani Fungicidresistans mot fluopyram hos Alternaria solani Ekdahl, Felicia Fluopyram Alternaria solani early blight potato Solanum tuberusum Early blight in potato production can be devastating and to minimize crop damage the use of fungicides has been the key. New fungicides called the SDHIs have been used against the early blight pathogen, Alternaria Solani in potato fields to reduce yield losses. However, repeated fungicide applications run the risk of fungicide resistance development in the fungal population, through the selection of mutations in the genes that are targeted by the fungicides. Mutations that confer a loss of sensitivity to the fungicide (i.e., fungicide resistance) will be selected for in fungal populations where there is a significant selection pressure, through for example, the repeated use of one class of fungicides. To investigate whether A. solani was becoming insensitive to the new SDHI fungicide fluopyram, a growth study was conducted where 20 different strains of A.solani were grown on solid medium with five different concentrations to see if some of the strains were less susceptible than others. It was difficult to draw in depth conclusions from this growth study, however some samples showed less sensitivity to the fluopyram. A DNA analysis of the fluopyram target gene was also performed to try to determine if there were any mutations associated with resistance present. However, due to problems with the PCR and sequencing experiments it was not possible to make any firm conclusions from this. SLU/Dept. of Plant Protection Biology 2022 H2 eng https://stud.epsilon.slu.se/17920/
spellingShingle Fluopyram
Alternaria solani
early blight
potato
Solanum tuberusum
Ekdahl, Felicia
Fungicide resistance against fluopyram in Alternaria solani
title Fungicide resistance against fluopyram in Alternaria solani
title_full Fungicide resistance against fluopyram in Alternaria solani
title_fullStr Fungicide resistance against fluopyram in Alternaria solani
title_full_unstemmed Fungicide resistance against fluopyram in Alternaria solani
title_short Fungicide resistance against fluopyram in Alternaria solani
title_sort fungicide resistance against fluopyram in alternaria solani
topic Fluopyram
Alternaria solani
early blight
potato
Solanum tuberusum