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The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)

The associations of fungal endophytes with trees are poorly understood, especially within tree seeds. Recent molecular studies show that tree seeds harbour highly diverse fungal communities, including potential plant pathogens that could be introduced to new areas when seeds are exchanged. Howeve...

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Main Author: Stolarek, Kinga
Format: Second cycle, A2E
Language:Swedish
Inglés
Published: 2022
Subjects:
Forestry production
Online Access:https://stud.epsilon.slu.se/17770/
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author Stolarek, Kinga
author_browse Stolarek, Kinga
author_facet Stolarek, Kinga
author_sort Stolarek, Kinga
collection Epsilon Archive for Student Projects
description The associations of fungal endophytes with trees are poorly understood, especially within tree seeds. Recent molecular studies show that tree seeds harbour highly diverse fungal communities, including potential plant pathogens that could be introduced to new areas when seeds are exchanged. However, only a small fraction of these fungi can be detected when traditional culturing is used. Traditional culturing is widely used for assessing fungal diversity in plant tissues because it allows the detection of viable fungi and provides pure isolates for future experiments. However, various nutrient media have been used for fungal culturing and it is unknown if the choice of nutrient media affects the captured diversity. This study assesses the overall diversity of endophytes in seeds of European beech (Fagus sylvatica L.) and tests the effect of media and seed origins on endophyte diversity by growing fungi from seeds from six locations on three commonly used types of media that differ in carbon sources (Malt Extract Agar, Potato Dextrose Agar and Water Agar). The results show that almost all tested seeds of European beech yielded fungi, and reveal high diversity of fungal endophytes, including plant pathogens. Captured diversity and community composition stayed unchanged irrespective of the medium type, but it differed across locations of seed origin. The results show that similar communities are obtained using three most commonly used media which indicates that most of the fungi are able to utilise simple and complex carbohydrates and allows comparison of diversity assessed by those media. The results also indicate that dangerous fungal pathogens could be moved within the seeds exchanged for tree reforestation purposes which calls for the use of local seeds for reforestation.
format Second cycle, A2E
id RepoSLU17770
institution Swedish University of Agricultural Sciences
language Swedish
Inglés
publishDate 2022
publishDateSort 2022
record_format eprints
spelling RepoSLU177702022-05-21T01:03:31Z https://stud.epsilon.slu.se/17770/ The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.) Stolarek, Kinga Forestry production The associations of fungal endophytes with trees are poorly understood, especially within tree seeds. Recent molecular studies show that tree seeds harbour highly diverse fungal communities, including potential plant pathogens that could be introduced to new areas when seeds are exchanged. However, only a small fraction of these fungi can be detected when traditional culturing is used. Traditional culturing is widely used for assessing fungal diversity in plant tissues because it allows the detection of viable fungi and provides pure isolates for future experiments. However, various nutrient media have been used for fungal culturing and it is unknown if the choice of nutrient media affects the captured diversity. This study assesses the overall diversity of endophytes in seeds of European beech (Fagus sylvatica L.) and tests the effect of media and seed origins on endophyte diversity by growing fungi from seeds from six locations on three commonly used types of media that differ in carbon sources (Malt Extract Agar, Potato Dextrose Agar and Water Agar). The results show that almost all tested seeds of European beech yielded fungi, and reveal high diversity of fungal endophytes, including plant pathogens. Captured diversity and community composition stayed unchanged irrespective of the medium type, but it differed across locations of seed origin. The results show that similar communities are obtained using three most commonly used media which indicates that most of the fungi are able to utilise simple and complex carbohydrates and allows comparison of diversity assessed by those media. The results also indicate that dangerous fungal pathogens could be moved within the seeds exchanged for tree reforestation purposes which calls for the use of local seeds for reforestation. 2022-05-18 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/17770/3/stolarek-k-20220520.pdf Stolarek, Kinga, 2022. The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.). Second cycle, A2E. Alnarp: (S) > Southern Swedish Forest Research Centre <https://stud.epsilon.slu.se/view/divisions/OID-295.html> urn:nbn:se:slu:epsilon-s-17770 eng
spellingShingle Forestry production
Stolarek, Kinga
The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title_full The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title_fullStr The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title_full_unstemmed The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title_short The effect of various nutrient media on the captured diversity of culturable seed endophytes in European beech (Fagus sylvatica L.)
title_sort effect of various nutrient media on the captured diversity of culturable seed endophytes in european beech (fagus sylvatica l.)
topic Forestry production
url https://stud.epsilon.slu.se/17770/
https://stud.epsilon.slu.se/17770/

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