Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa
In the year 2000, the National program for diversity of cultivated plants (Pom) was established, aiming to recover old, Swedish fruit and berry cultivars that historically have been important for breeding and commercial production. A collection of mandate cultivars of Ribes uva-crispa (gooseberry) c...
| Autor principal: | |
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2021
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| Acceso en línea: | https://stud.epsilon.slu.se/17432/ |
| _version_ | 1855572896883146752 |
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| author | Nordlander, Josefine |
| author_browse | Nordlander, Josefine |
| author_facet | Nordlander, Josefine |
| author_sort | Nordlander, Josefine |
| collection | Epsilon Archive for Student Projects |
| description | In the year 2000, the National program for diversity of cultivated plants (Pom) was established, aiming to recover old, Swedish fruit and berry cultivars that historically have been important for breeding and commercial production. A collection of mandate cultivars of Ribes uva-crispa (gooseberry) containing valuable accessions was created. Currently, 16 accessions from the mandate list are missing in the collection and missing mandate cultivars could be found through a national inventory of plant resources. In this study, mandate cultivars and inventory findings collected by Pom, were analyzed though molecular characterization (SSR-markers), to find duplicates, mislabelings and to analyze the genetic variability of the collection.
The present work uncovered several cases of duplication among the inventory findings and one, possible case of mislabeling. All the mandate cultivars had unique SSR-profiles, which was expected. Furthermore, of the 15 SSR markers used, 6 markers are particularly effective at describing the variability of the gooseberry germplasm. The PCoA analysis revealed the presence of a relatively more homogenous group among the analyzed plant material. These accessions display more genetic similarities, compared to the rest of the analyzed plants.
The data constitutes an important basis for evaluation and addition of accessions to the present collection of mandate cultivars and for removal of duplicates among the inventory findings. |
| format | Second cycle, A2E |
| id | RepoSLU17432 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2021 |
| publishDateSort | 2021 |
| record_format | eprints |
| spelling | RepoSLU174322021-12-17T05:03:50Z https://stud.epsilon.slu.se/17432/ Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa Nordlander, Josefine Plant genetics and breeding In the year 2000, the National program for diversity of cultivated plants (Pom) was established, aiming to recover old, Swedish fruit and berry cultivars that historically have been important for breeding and commercial production. A collection of mandate cultivars of Ribes uva-crispa (gooseberry) containing valuable accessions was created. Currently, 16 accessions from the mandate list are missing in the collection and missing mandate cultivars could be found through a national inventory of plant resources. In this study, mandate cultivars and inventory findings collected by Pom, were analyzed though molecular characterization (SSR-markers), to find duplicates, mislabelings and to analyze the genetic variability of the collection. The present work uncovered several cases of duplication among the inventory findings and one, possible case of mislabeling. All the mandate cultivars had unique SSR-profiles, which was expected. Furthermore, of the 15 SSR markers used, 6 markers are particularly effective at describing the variability of the gooseberry germplasm. The PCoA analysis revealed the presence of a relatively more homogenous group among the analyzed plant material. These accessions display more genetic similarities, compared to the rest of the analyzed plants. The data constitutes an important basis for evaluation and addition of accessions to the present collection of mandate cultivars and for removal of duplicates among the inventory findings. Under år 2000 grundades Programmet för odlad mångfald, (Pom), med syftet att bevara gamla kultursorter av bland annat frukt och bär. För att en sort ska bevaras behöver den dels inneha ett kulturhistoriskt värde, dels måste sorten ha varit viktig för Sverige antingen i förädlingssammanhang eller i kommersiell produktion. På denna grund valde man ut ett antal krusbärssorter som fick benämningen mandatsorter och de sorter som kunde hittas och valideras fick ingå i en samling i Alnarp (SLU). För tillfället saknas dock 16 accessioner från mandatlistan i samlingen. Äldre, saknade sorter kan hittas genom nationella inventeringar, där man samlar in intressant växtmaterial för analys. I denna studie genomfördes en molekylär karaktärisering med hjälp av SSR-markörer av mandatsorterna i samlingen och de inventeringsfynd som Pom samlat in. Målet med analysen var att hitta duplikat eller felmärkningar samt analysera samlingens genetiska variabilitet. Studien visade att det fanns ett flertal duplikat bland inventeringsfynden och ett fall av felmärkning. Alla mandatsorter hade unika SSR-profiler, vilket var förväntat. Det visade sig också att av de 15 SSR markörer som användes, så var 6 markörer särskilt effektiva för att beskriva variabiliteten i arvsmassan hos krusbär. PCoA analysen visade att det fanns en relativt homogen grupp bland det analyserade växtmaterialet. Dessa accessioner visade på större genetiska likheter i jämförelse med resten av de analyserade krusbärsbuskarna. Resultaten från denna studie kan ligga till grund för utvärdering av samlingen av inventeringsfynd, borttagning av duplikat och eventuell addering av accessioner till den nuvarande samlingen av mandatsorter. 2021-12-14 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/17432/1/nordlander_j_211214.pdf Nordlander, Josefine, 2021. Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa. Second cycle, A2E. Alnarp: (LTJ, LTV) > Dept. of Plant Breeding (from 130101) <https://stud.epsilon.slu.se/view/divisions/4818.html> urn:nbn:se:slu:epsilon-s-17432 eng |
| spellingShingle | Plant genetics and breeding Nordlander, Josefine Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title | Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title_full | Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title_fullStr | Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title_full_unstemmed | Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title_short | Genetic diversity within Swedish national heritage cultivars of gooseberry, Ribes uva-crispa |
| title_sort | genetic diversity within swedish national heritage cultivars of gooseberry, ribes uva-crispa |
| topic | Plant genetics and breeding |
| url | https://stud.epsilon.slu.se/17432/ https://stud.epsilon.slu.se/17432/ |