Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden
Rust fungi belong to the phylum Basidiomycetes, order Uredinales cause damage on almost all plants, including cereal crops. Some of the species within the genus Puccinia causes rust disease on cereal crops. One example of a disease from this genus is Puccinia graminis, which historically has caused...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2021
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| Acceso en línea: | https://stud.epsilon.slu.se/17331/ |
| _version_ | 1855572882982174720 |
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| author | Kjellström, Clara |
| author_browse | Kjellström, Clara |
| author_facet | Kjellström, Clara |
| author_sort | Kjellström, Clara |
| collection | Epsilon Archive for Student Projects |
| description | Rust fungi belong to the phylum Basidiomycetes, order Uredinales cause damage on almost all plants, including cereal crops. Some of the species within the genus Puccinia causes rust disease on cereal crops. One example of a disease from this genus is Puccinia graminis, which historically has caused severe damage in cereal crop production. During recent years, P. graminis have re-emerged in Sweden on barley and wheat. The aim of this work was to examine if the same population of P. graminis infects both wheat and barley in Sweden. In total 204 samples were collected of the crops, 123 from barley (Hordeum vulgare), 10 from rye (Secale cereale) samples and 71 from wheat (Triticum aestivum) in 16 farmers’ fields. The genotypic diversity was examined based on microsatellite markers and the population structure and genetic relationship within and between the collected samples were analysed.
The results indicate that the P. graminis population on barley and wheat seemed to be partially divided in two clusters. However, the clusters were partially overlapping, and the main branches of barley and wheat had smaller branches of barley and wheat within them. The rye samples were spread mainly in the barley cluster or branch, but more samples are needed to draw conclusions about the rye population. This could indicate that the P. graminis population on barley and wheat are not fully genetically separated consisting of a meta-population, but that there probably is a selection on the different cereal hosts. The result did also confirm that there is a high genetic diversity within the populations, indicating that sexual recombination can be completed on the aecial host Berberis spp. in Sweden. There was no correlation with geographic and genetic distance, confirming that there is mostly wind dispersal of P. graminis. |
| format | Second cycle, A2E |
| id | RepoSLU17331 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2021 |
| publishDateSort | 2021 |
| record_format | eprints |
| spelling | RepoSLU173312021-10-29T01:02:16Z https://stud.epsilon.slu.se/17331/ Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden Kjellström, Clara Agricultural research Protection of plants - General aspects Plant diseases Rust fungi belong to the phylum Basidiomycetes, order Uredinales cause damage on almost all plants, including cereal crops. Some of the species within the genus Puccinia causes rust disease on cereal crops. One example of a disease from this genus is Puccinia graminis, which historically has caused severe damage in cereal crop production. During recent years, P. graminis have re-emerged in Sweden on barley and wheat. The aim of this work was to examine if the same population of P. graminis infects both wheat and barley in Sweden. In total 204 samples were collected of the crops, 123 from barley (Hordeum vulgare), 10 from rye (Secale cereale) samples and 71 from wheat (Triticum aestivum) in 16 farmers’ fields. The genotypic diversity was examined based on microsatellite markers and the population structure and genetic relationship within and between the collected samples were analysed. The results indicate that the P. graminis population on barley and wheat seemed to be partially divided in two clusters. However, the clusters were partially overlapping, and the main branches of barley and wheat had smaller branches of barley and wheat within them. The rye samples were spread mainly in the barley cluster or branch, but more samples are needed to draw conclusions about the rye population. This could indicate that the P. graminis population on barley and wheat are not fully genetically separated consisting of a meta-population, but that there probably is a selection on the different cereal hosts. The result did also confirm that there is a high genetic diversity within the populations, indicating that sexual recombination can be completed on the aecial host Berberis spp. in Sweden. There was no correlation with geographic and genetic distance, confirming that there is mostly wind dispersal of P. graminis. Växtsjukdomar orsakade av rostsvampar vilka tillhör fylumet Basidiomyceter och ordningen Urdinales orsakar skador på nästan alla växter inklusive spannmålsgrödor. Ett släkte som orsakar rostsjukdomar på våra spannmålsgrödor är Puccinia spp., och ett exempel en patogen i detta släkte är Puccinia graminis som historiskt orsakat stora skador på spannmålsgrödor. Under de senaste åren har infektioner av svartrost orsakade av P. graminis noterats på vete, korn och råg på flera platser i Sverige. Syftet med detta examensarbete är att undersöka om det är samma population av P. graminis som både infekterar vete och korn i Sverige. Totalt samlades 204 prov in a grödorna, 123 från korn (Hordeum vulgare), 10 prov från råg (Secale cereale) och 71 från vete (Triticum aestivum) i 16 fält. Den genotypiska diversiteten bestämdes med hjälp av mikrosattelitmarkörer och populationsstrukturen inom och mellan de insamlade proverna analyserades. Resultaten visar att populationer av P. graminis verkade vara uppdelade i två grenar eller kluster, men dessa var inte helt separerade. Huvudgrenen av korn och vete hade en mindre gren av vete eller korn inom sig. Svamppopulationen insamlad på råg var spridd främst inom kornklustret, dock behöver mer prover för att kunna dra säkra slutsatser om prover från just råg. Detta tyder på att populationerna av P. graminis inte är genetiskt separerade utan består av en meta-population, men att det troligtvis sker en selektion på de olika värdarna vete, korn och råg. Resultaten bekräftade även att P. graminis hade hög genetisk diversitet, vilket tyder på sexuell rekombination kan slutföras på den alternativa värden Berberis spp. Det fanns inget samband mellan geografiskt och genetiskt avstånd, vilket tyder på att P. graminis är huvudsakligen vindspridd. 2021-10-20 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/17331/1/kjellstrom_C_211020.pdf Kjellström, Clara, 2021. Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden. Second cycle, A2E. Uppsala: (NL, NJ) > Dept. of Forest Mycology and Plant Pathology <https://stud.epsilon.slu.se/view/divisions/OID-390.html> urn:nbn:se:slu:epsilon-s-17331 eng |
| spellingShingle | Agricultural research Protection of plants - General aspects Plant diseases Kjellström, Clara Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title | Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title_full | Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title_fullStr | Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title_full_unstemmed | Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title_short | Population structure of Puccinia graminis, the cause of stem rust on wheat, barley, and rye in Sweden |
| title_sort | population structure of puccinia graminis, the cause of stem rust on wheat, barley, and rye in sweden |
| topic | Agricultural research Protection of plants - General aspects Plant diseases |
| url | https://stud.epsilon.slu.se/17331/ https://stud.epsilon.slu.se/17331/ |