Urea’s ability to break the life cycle of Nematodirus spp. in situ

Nematodirus spp., along with other gastrointestinal nematodes, are a common cause of disease among sheep and remains a major animal health concern, and cause of reduced productivity for sheep farmers around the world. As the resistance against anthelmintics is substantial among gastrointestinal n...

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Autor principal: Falkman, Carolina
Formato: Second cycle, A2E
Lenguaje:sueco
Inglés
Publicado: 2020
Materias:
Acceso en línea:https://stud.epsilon.slu.se/15726/
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author Falkman, Carolina
author_browse Falkman, Carolina
author_facet Falkman, Carolina
author_sort Falkman, Carolina
collection Epsilon Archive for Student Projects
description Nematodirus spp., along with other gastrointestinal nematodes, are a common cause of disease among sheep and remains a major animal health concern, and cause of reduced productivity for sheep farmers around the world. As the resistance against anthelmintics is substantial among gastrointestinal nematodes, there is an urgent need of significant alternatives to anthelmintic treatment. The life cycle of Nematodirus comprises stages both within and outside the host, with the pre-parasitic development to the infective third stage, occurring on pasture. With evidence suggesting that nitrogenous fertilisers could have the ability to inhibit egg hatching and larval survival in numerous gastrointestinal nematodes, the aim of this study was to investigate urea’s ability, in situ, to break the life cycle of Nematodirus spp. outside of the host. The method used was through targeted application of liquid urea to experimental pastures at times when Nematodirus eggs are present on pasture. The study included seven experimental paddocks previously grazed by sheep with a known infestation of Nematodirus spp. and other Strongyles. The sheep were of different genetic lines, bred for either resistance or resilience against gastrointestinal nematodes. The application of liquid urea involved five repeated sprayings of 40kg N/ha every three weeks before the start of grazing season, or one single spraying of 200kg N/ha three months before start of grazing, with control paddocks left untreated. The effect of treatments was evaluated utilising three methods, i.e. observing pasture larval concentration, faecal egg counts and concentration of eggs and larvae in soil. The study had a main focus on Nematodirus spp., although “other” Strongyles were included in the results to give a better understanding of the overall effects of the treatments. Overall the results were equivocal, with absence of consistently statistically significant differences between treatments and control. However not significant, although a subject for discussion, was that urea could have a stimulating effect on egg hatching and larval development. In conclusion, the results from this field trial have demonstrated insufficient evidence that nitrogenous fertilisers provide epidemiological benefits in reducing larval contamination on pasture. It has been demonstrated that it is challenging to translate the in vitro results into the field, with the parasite having complex hatching requirements arising of the environment, thus difficult to influence.
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spelling RepoSLU157262020-07-07T01:01:39Z https://stud.epsilon.slu.se/15726/ Urea’s ability to break the life cycle of Nematodirus spp. in situ Falkman, Carolina Veterinary science and hygiene - General aspects Animal diseases Nematodirus spp., along with other gastrointestinal nematodes, are a common cause of disease among sheep and remains a major animal health concern, and cause of reduced productivity for sheep farmers around the world. As the resistance against anthelmintics is substantial among gastrointestinal nematodes, there is an urgent need of significant alternatives to anthelmintic treatment. The life cycle of Nematodirus comprises stages both within and outside the host, with the pre-parasitic development to the infective third stage, occurring on pasture. With evidence suggesting that nitrogenous fertilisers could have the ability to inhibit egg hatching and larval survival in numerous gastrointestinal nematodes, the aim of this study was to investigate urea’s ability, in situ, to break the life cycle of Nematodirus spp. outside of the host. The method used was through targeted application of liquid urea to experimental pastures at times when Nematodirus eggs are present on pasture. The study included seven experimental paddocks previously grazed by sheep with a known infestation of Nematodirus spp. and other Strongyles. The sheep were of different genetic lines, bred for either resistance or resilience against gastrointestinal nematodes. The application of liquid urea involved five repeated sprayings of 40kg N/ha every three weeks before the start of grazing season, or one single spraying of 200kg N/ha three months before start of grazing, with control paddocks left untreated. The effect of treatments was evaluated utilising three methods, i.e. observing pasture larval concentration, faecal egg counts and concentration of eggs and larvae in soil. The study had a main focus on Nematodirus spp., although “other” Strongyles were included in the results to give a better understanding of the overall effects of the treatments. Overall the results were equivocal, with absence of consistently statistically significant differences between treatments and control. However not significant, although a subject for discussion, was that urea could have a stimulating effect on egg hatching and larval development. In conclusion, the results from this field trial have demonstrated insufficient evidence that nitrogenous fertilisers provide epidemiological benefits in reducing larval contamination on pasture. It has been demonstrated that it is challenging to translate the in vitro results into the field, with the parasite having complex hatching requirements arising of the environment, thus difficult to influence. 2020-06-29 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/15726/1/falkman_c_200121.pdf Falkman, Carolina, 2020. Urea’s ability to break the life cycle of Nematodirus spp. in situ : a field study on sheep in New Zealand. Second cycle, A2E. Uppsala: (VH) > Dept. of Clinical Sciences (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-715.html> urn:nbn:se:slu:epsilon-s-15726 eng
spellingShingle Veterinary science and hygiene - General aspects
Animal diseases
Falkman, Carolina
Urea’s ability to break the life cycle of Nematodirus spp. in situ
title Urea’s ability to break the life cycle of Nematodirus spp. in situ
title_full Urea’s ability to break the life cycle of Nematodirus spp. in situ
title_fullStr Urea’s ability to break the life cycle of Nematodirus spp. in situ
title_full_unstemmed Urea’s ability to break the life cycle of Nematodirus spp. in situ
title_short Urea’s ability to break the life cycle of Nematodirus spp. in situ
title_sort urea’s ability to break the life cycle of nematodirus spp. in situ
topic Veterinary science and hygiene - General aspects
Animal diseases
url https://stud.epsilon.slu.se/15726/
https://stud.epsilon.slu.se/15726/