The mechanisms of TK1 secretion in cancer cells
A seventy five percent increase in cancer incidence within 10 years is one of the major human health issues based on the World Health Or-ganization (WHO) evaluation. Prevention of cancer through health screening was suggested by the WHO as a means of early detection of cancer. Thymidine kinase 1 (TK...
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| Formato: | H2 |
| Lenguaje: | Inglés |
| Publicado: |
SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231)
2019
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| Materias: |
| _version_ | 1855572537096798208 |
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| author | Mohajer Shojai, Tabassom |
| author_browse | Mohajer Shojai, Tabassom |
| author_facet | Mohajer Shojai, Tabassom |
| author_sort | Mohajer Shojai, Tabassom |
| collection | Epsilon Archive for Student Projects |
| description | A seventy five percent increase in cancer incidence within 10 years is one of the major human health issues based on the World Health Or-ganization (WHO) evaluation. Prevention of cancer through health screening was suggested by the WHO as a means of early detection of cancer. Thymidine kinase 1 (TK1) is one of the key enzymes for DNA replication and cell division, which could have a role in tumor for-mation and progression. Exosomes are extracellular vesicles which were recently shown to act as messengers of tumor cells to communi-cate with cells in the body. The aim of this study was to evaluate if TK1 was excreted via exosomes in a BJ tumor (BJ-T) cell line as well as BJ normal (BJ-N) cell line. Exosomes were isolated from media superna-tants of BJ-T and BJ-N cells using ultracentrifugation and the final exo-some preparations were resuspended in phosphate buffered saline. TK1 activity and concentration in the supernatants before and after ultracen-trifugation as well as exosome samples were evaluated by [3H]-dThd phosphorylation assay and dot-blot chemiluminescence assay, respec-tively. Specific activity of BJ-T exosome-derived TK1 was 16.9 times more than BJ-N exosome-derived TK1, where there was also two times more TK1 concentration in BJ-T exosomes compared to BJ-N exo-somes. In conclusion, TK1 excretion was via exosomes and exosome-derived TK1 could be a tumor biomarker for BJ-T cells. Furthermore in vitro and in vivo investigations are needed in order to evaluate whether TK1 could be a useful biomarker for tumor tissue and/or in clinical level. |
| format | H2 |
| id | RepoSLU15182 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| publisherStr | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| record_format | eprints |
| spelling | RepoSLU151822019-11-08T15:15:55Z The mechanisms of TK1 secretion in cancer cells Mohajer Shojai, Tabassom Thymidine kinase 1 excretion cancer cell line dot-blot chemiluminescence assay exosome isola-tion [3H]-dThd phosphorylation assay A seventy five percent increase in cancer incidence within 10 years is one of the major human health issues based on the World Health Or-ganization (WHO) evaluation. Prevention of cancer through health screening was suggested by the WHO as a means of early detection of cancer. Thymidine kinase 1 (TK1) is one of the key enzymes for DNA replication and cell division, which could have a role in tumor for-mation and progression. Exosomes are extracellular vesicles which were recently shown to act as messengers of tumor cells to communi-cate with cells in the body. The aim of this study was to evaluate if TK1 was excreted via exosomes in a BJ tumor (BJ-T) cell line as well as BJ normal (BJ-N) cell line. Exosomes were isolated from media superna-tants of BJ-T and BJ-N cells using ultracentrifugation and the final exo-some preparations were resuspended in phosphate buffered saline. TK1 activity and concentration in the supernatants before and after ultracen-trifugation as well as exosome samples were evaluated by [3H]-dThd phosphorylation assay and dot-blot chemiluminescence assay, respec-tively. Specific activity of BJ-T exosome-derived TK1 was 16.9 times more than BJ-N exosome-derived TK1, where there was also two times more TK1 concentration in BJ-T exosomes compared to BJ-N exo-somes. In conclusion, TK1 excretion was via exosomes and exosome-derived TK1 could be a tumor biomarker for BJ-T cells. Furthermore in vitro and in vivo investigations are needed in order to evaluate whether TK1 could be a useful biomarker for tumor tissue and/or in clinical level. SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) 2019 H2 eng https://stud.epsilon.slu.se/15182/ |
| spellingShingle | Thymidine kinase 1 excretion cancer cell line dot-blot chemiluminescence assay exosome isola-tion [3H]-dThd phosphorylation assay Mohajer Shojai, Tabassom The mechanisms of TK1 secretion in cancer cells |
| title | The mechanisms of TK1 secretion in cancer cells |
| title_full | The mechanisms of TK1 secretion in cancer cells |
| title_fullStr | The mechanisms of TK1 secretion in cancer cells |
| title_full_unstemmed | The mechanisms of TK1 secretion in cancer cells |
| title_short | The mechanisms of TK1 secretion in cancer cells |
| title_sort | mechanisms of tk1 secretion in cancer cells |
| topic | Thymidine kinase 1 excretion cancer cell line dot-blot chemiluminescence assay exosome isola-tion [3H]-dThd phosphorylation assay |