Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple
Texture, firmness and storability are among the most important traits for fruit quality in apple. Consumers are increasingly demanding apples with a “crispy texture”, and the market is offering a premium for a longer shelf life, which has as a result generated an increased interest from plant breede...
| Main Author: | |
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| Format: | First cycle, G2E |
| Language: | Swedish Inglés |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://stud.epsilon.slu.se/14817/ |
| _version_ | 1855572475143782400 |
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| author | Lundmark, Johan |
| author_browse | Lundmark, Johan |
| author_facet | Lundmark, Johan |
| author_sort | Lundmark, Johan |
| collection | Epsilon Archive for Student Projects |
| description | Texture, firmness and storability are among the most important traits for fruit quality in apple. Consumers are increasingly demanding apples with a “crispy texture”, and the market is offering a premium for a longer shelf life, which has as a result generated an increased interest from plant breeders to meet these demands. The traits are internally regulated by the fruit‟s inherent ethylene production, the suppression of which results in higher firmness retention. Thus far, the ethylene production genes Md-ACS1 and Md-ACO1 represent the best known candidates for enhancing firmness retention during storage. In this study, capillary electrophoresis was used to screen 255 cultivars from Nordic and Baltic germplasm collections for alleles at the Md-ACS1 and Md-ACO1 loci. This was done in order to enable DNA-marker based selections of these genes in breeding material suitable for cool climates. In addition to compiling genotype data, we performed verifications of previously screened cultivars, and additional evaluations of parentage were made in order to determine any pedigree errors. The analysis of the Md-ACO1 locus showed false positives for uncertain reasons, and therefore the true allelic compositions could not be determined. Frequencies of the normal ethylene allele Md-ACS1-1 was much higher in the Estonian (0.94), Finnish (0.95) and Latvian (0.90) collections, whereas the low ethylene allele ACS1-2 was more common in the Lithuanian (0.22), Swedish (0.33) and Norwegian (0.35) collections. Of the previously screened cultivars, 59 showed the expected genotype, with the cultivar „EvaLotta‟ constituting the only exception. In addition, this cultivar was the only genotype that showed any inconsistencies when comparing it to the presumed parents. |
| format | First cycle, G2E |
| id | RepoSLU14817 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2019 |
| publishDateSort | 2019 |
| record_format | eprints |
| spelling | RepoSLU148172020-05-29T13:15:35Z https://stud.epsilon.slu.se/14817/ Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple Lundmark, Johan Plant genetics and breeding Texture, firmness and storability are among the most important traits for fruit quality in apple. Consumers are increasingly demanding apples with a “crispy texture”, and the market is offering a premium for a longer shelf life, which has as a result generated an increased interest from plant breeders to meet these demands. The traits are internally regulated by the fruit‟s inherent ethylene production, the suppression of which results in higher firmness retention. Thus far, the ethylene production genes Md-ACS1 and Md-ACO1 represent the best known candidates for enhancing firmness retention during storage. In this study, capillary electrophoresis was used to screen 255 cultivars from Nordic and Baltic germplasm collections for alleles at the Md-ACS1 and Md-ACO1 loci. This was done in order to enable DNA-marker based selections of these genes in breeding material suitable for cool climates. In addition to compiling genotype data, we performed verifications of previously screened cultivars, and additional evaluations of parentage were made in order to determine any pedigree errors. The analysis of the Md-ACO1 locus showed false positives for uncertain reasons, and therefore the true allelic compositions could not be determined. Frequencies of the normal ethylene allele Md-ACS1-1 was much higher in the Estonian (0.94), Finnish (0.95) and Latvian (0.90) collections, whereas the low ethylene allele ACS1-2 was more common in the Lithuanian (0.22), Swedish (0.33) and Norwegian (0.35) collections. Of the previously screened cultivars, 59 showed the expected genotype, with the cultivar „EvaLotta‟ constituting the only exception. In addition, this cultivar was the only genotype that showed any inconsistencies when comparing it to the presumed parents. Textur, fasthet och lagringsförmåga är bland de mest avgörande egenskaperna för ett äpples fruktkvalitet. Konsumenter kräver alltmer "krispig konsistens" i sina äpplen, och marknaden erbjuder högre pris för längre hållbarhet. Detta har resulterat i ett ökat intresse från växtförädlare för att möta kraven som ställs. Kvalitetskraven påverkas av äpplets egna etenproduktion, som vid nedreglering ökar äpplets förmåga att bevara fasthet vid lagring. Hittills representerar etenproduktionsgenerna Md-ACS1 och Md-ACO1 de bästa kandidaterna för att förbättra lagringsförmågan. I den här studien undersöktes Md-ACS1 och MdACO1 alleler i 255 sorter från nordiska och baltiska samlingar, med hjälp av kapillärelektrofores. Detta gjordes för att möjliggöra DNA-markörbaserade selekteringar för dessa gener i sorter som är lämpliga för kalla klimat. Förutom att sammanställa genotypdata utförde vi verifieringar av tidigare undersökta sorter. Ytterligare utvärderingar av föräldraskap gjordes för att avgöra eventuellt felbestämda härkomster. Av osäkra skäl visade analysen falskt positiva resultat i Md-ACO1 locuset och allelsammansättningen kunde därför inte bestämmas korrekt. Frekvenserna för allelen Md-ACS1-1, som är associerad med normal etenproduktion, var mycket högre i de estniska (0,94), finska (0,95) och lettiska (0,90) samlingarna, medan Md-ACS1-2 allelen, associerad med låg etenproduktion, var vanligare i litauiska (0,22), svenska (0,33) och norska (0,35) samlingar. Av de tidigare undersökta sorterna upvisade 59 den förväntade genotypen. Sorten "Eva-Lotta" utgjorde det enda undantaget. Dessutom var "Eva-Lotta" den enda sort vars genotyp inte överensstämde med de kända föräldrarnas genotyper. 2019-07-10 First cycle, G2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/14817/7/lundmark_j_190708.pdf Lundmark, Johan, 2019. Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple. First cycle, G2E. Alnarp: (LTJ, LTV) > Dept. of Plant Breeding (from 130101) <https://stud.epsilon.slu.se/view/divisions/4818.html> urn:nbn:se:slu:epsilon-s-10753 eng |
| spellingShingle | Plant genetics and breeding Lundmark, Johan Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title | Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title_full | Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title_fullStr | Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title_full_unstemmed | Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title_short | Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple |
| title_sort | genotyping ethylene production genes md-acs1 and md-aco1 for markerassisted selection in apple |
| topic | Plant genetics and breeding |
| url | https://stud.epsilon.slu.se/14817/ https://stud.epsilon.slu.se/14817/ |