Evaluation of GM trees expressing a fungal enzyme
The fungi enzyme glucuronoyl esterase can be used to change the cell wall composition in plants for easier extraction of cellulosic biomass compounds for the production of biomaterials and biofuels. However, previous studies have shown that expression of glucuronoyl esterase in plants causes stress...
| Autores principales: | , |
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| Formato: | First cycle, G2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/14766/ |
| Sumario: | The fungi enzyme glucuronoyl esterase can be used to change the cell wall composition in plants for easier extraction of cellulosic biomass compounds for the production of biomaterials and biofuels. However, previous studies have shown that expression of glucuronoyl esterase in plants causes stress response, mediated by jasmonate signaling, and growth penalty. In this study, the GUS reporter system for jasmonate signaling was used to test if the transgenic plants react to the activity of glucuronoyl esterase or to the presence of foreign protein.
Arabidopsis line expressing JAZ10:GUS construct was used to test effects of glucuronoyl esterase applications on seven days old seedlings. The results obtained indicate that the fungal enzyme is recognized by the plant as pathogenic. The hypothesis that the activity contributes to the stress response cannot be rejected, therefore, further testing is needed to prove that. It was also concluded that the experiment require high number of plants samples for clear results, and that small disturbances of the seedlings can induce temporary stress response. Therefore, the time lag period between the selection of seedlings and the application of the enzyme should be at least 24 hours to avoid unwanted stress response. |
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