Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus)
The total number of goats (Capra hircus) in Sweden is small compared to the Swedish population of cattle and the Norwegian population of goats. Cheese making from goat milk is one of the productions done in Sweden, even though Swedish goats have a low percentage of caseins in the milk (2.1 %) compar...
| Autor principal: | |
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2019
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| Acceso en línea: | https://stud.epsilon.slu.se/14201/ |
| _version_ | 1855572371256115200 |
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| author | Björk, Agnes |
| author_browse | Björk, Agnes |
| author_facet | Björk, Agnes |
| author_sort | Björk, Agnes |
| collection | Epsilon Archive for Student Projects |
| description | The total number of goats (Capra hircus) in Sweden is small compared to the Swedish population of cattle and the Norwegian population of goats. Cheese making from goat milk is one of the productions done in Sweden, even though Swedish goats have a low percentage of caseins in the milk (2.1 %) compared to Nubian goats. Caseins are one factor that affect the produced cheese yield from milk. There are four different caseins; αs1-casein (αs1-CN), αS2-casein (αs2-CN), β-casein and κ-casein. Their respective coding genes are CSN1S1, CSN1S2, CSN2 and CSN3. This study focused on αs1-CN and CSN1S1 with the exon 12 due to a deletion in this exon that has been reported at a high frequency (67.6 %) in Norwegian goats. Forty-eight individual goats of the breeds Swedish Landrace goat, Jämt goat, Lapp goat and Göinge goat from all around Sweden were sequenced for exon 12 to be analysed if they carry the Norwegian deletion. In addition, milk samples from some of these goats and bulk milk have been analysed for the proportions of proteins and caseins. The results showed that the deletion did exist in all the Swedish breeds mentioned above and the allele frequency for the deletion was 94.1 %. The alleles G’ and A’ were also detected in the target region and the analysed milk samples showed that 53 % had low expression. Overall, this study gives insights about the deletion occurred at exon 12 of the CSN1S1, which affects the αs1-CN protein. |
| format | Second cycle, A2E |
| id | RepoSLU14201 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2019 |
| publishDateSort | 2019 |
| record_format | eprints |
| spelling | RepoSLU142012024-10-31T12:49:13Z https://stud.epsilon.slu.se/14201/ Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) Björk, Agnes Animal husbandry Animal genetics and breeding The total number of goats (Capra hircus) in Sweden is small compared to the Swedish population of cattle and the Norwegian population of goats. Cheese making from goat milk is one of the productions done in Sweden, even though Swedish goats have a low percentage of caseins in the milk (2.1 %) compared to Nubian goats. Caseins are one factor that affect the produced cheese yield from milk. There are four different caseins; αs1-casein (αs1-CN), αS2-casein (αs2-CN), β-casein and κ-casein. Their respective coding genes are CSN1S1, CSN1S2, CSN2 and CSN3. This study focused on αs1-CN and CSN1S1 with the exon 12 due to a deletion in this exon that has been reported at a high frequency (67.6 %) in Norwegian goats. Forty-eight individual goats of the breeds Swedish Landrace goat, Jämt goat, Lapp goat and Göinge goat from all around Sweden were sequenced for exon 12 to be analysed if they carry the Norwegian deletion. In addition, milk samples from some of these goats and bulk milk have been analysed for the proportions of proteins and caseins. The results showed that the deletion did exist in all the Swedish breeds mentioned above and the allele frequency for the deletion was 94.1 %. The alleles G’ and A’ were also detected in the target region and the analysed milk samples showed that 53 % had low expression. Overall, this study gives insights about the deletion occurred at exon 12 of the CSN1S1, which affects the αs1-CN protein. Det totala antalet getter (Capra hircus) i Sverige är lågt jämfört med den svenska populationen av nötkreatur och den norska populationen av getter. Ostproduktion av getmjölk är en av de produktioner man använder getter till i Sverige, även om svenska getter har en låg procentandel av kasein i mjölken (2,1%) jämfört med nubiska getter. Kaseiner är en faktor som påverkar ostutbytet från mjölk. Det finns fyra olika kaseiner; αs1-kasein (αs1-CN), αs2-kasein (αs2-CN), β-kasein och κ-kasein. Deras respektive kodande gener är CSN1S1, CSN1S2, CSN2 och CSN3. Denna studie fokuserade på αs1-CN och CSN1S1 med exon 12 på grund av en deletion i detta exon som har rapporterats vid en hög frekvens (67,6%) i norska getter. Ett antal av 48 enskilda getter av rasen svensk lantrasget, Jämtget, Lappget och Göingeget från hela Sverige har sekvenserats för exon 12 och analyseras för om de har den norska deletionen. Dessutom har mjölkprov från några av dessa getter och tankmjölk analyserats för proportionerna av proteiner och kaseiner. Resultaten visade att deletionen fanns i alla ovan nämnda svenska raser och allelfrekvensen för deletionen var 94,1%. Allelerna G' och A' upptäcktes även i målområdet och de analyserade mjölkproverna visade att 53% hade lågt uttryck. Sammantaget ger denna studie insikter om deletionen i exon 12 av CSN1S1 som påverkar as1-CN-proteinet. 2019-02-13 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/14201/7/Bjork_A_181219.pdf Björk, Agnes, 2018. Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus). Second cycle, A2E. Uppsala: (VH) > Dept. of Animal Breeding and Genetics (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-670.html> urn:nbn:se:slu:epsilon-s-10195 eng |
| spellingShingle | Animal husbandry Animal genetics and breeding Björk, Agnes Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title | Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title_full | Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title_fullStr | Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title_full_unstemmed | Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title_short | Detection of mutational sites in the CSN1S1 gene and analysis of αs1-casein composition of the milk in Swedish goats (Capra hircus) |
| title_sort | detection of mutational sites in the csn1s1 gene and analysis of αs1-casein composition of the milk in swedish goats (capra hircus) |
| topic | Animal husbandry Animal genetics and breeding |
| url | https://stud.epsilon.slu.se/14201/ https://stud.epsilon.slu.se/14201/ |