Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses?
Standardbred horses (SBs) are used in harness racing. Their common breeding history with Coldblooded trotters (CBTs) led us to the hypothesis, that the same region associated with race performance in CBTs, may be linked to the performance of SBs as well. EDN3 SNP (g.22:45748491C>T), was found to be...
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| Format: | Second cycle, A2E |
| Language: | Swedish Inglés |
| Published: |
2018
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| Online Access: | https://stud.epsilon.slu.se/13879/ |
| _version_ | 1855572316959801344 |
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| author | Novácková, Linda |
| author_browse | Novácková, Linda |
| author_facet | Novácková, Linda |
| author_sort | Novácková, Linda |
| collection | Epsilon Archive for Student Projects |
| description | Standardbred horses (SBs) are used in harness racing. Their common breeding history with Coldblooded trotters (CBTs) led us to the hypothesis, that the same region associated with race performance in CBTs, may be linked to the performance of SBs as well. EDN3 SNP (g.22:45748491C>T), was found to be associated (p<0.05) with performance traits in CBTs (Fegraeus, 2017). EDN3 SNP has a high frequency of the T-allele in breeds related to races and to the high performance (Fegraeus, 2017). EDN3 SNP is located in the vicinity of endothelin-3 (EDN3) gene. Protein endothelin-3 (EDN3) is responsible for the development of the enteric nervous system and the melanin distribution in the foetus and it is involved in the blood regulation. Custom designed TaqMan SNP Genotyping Assays (Applied Biosystems StepOnePlusTM Instrument by Life Technologies) were used to genotype 550 SBs. Four hundred and fifty-seven genotypes were obtained and no statistically significant association between racing performance traits and genotype was found. Several associations (<0.05) between performance traits and other fixed effects – sex and year of birth - were found. The T-allele frequency of EDN3 SNP is 0.87 and the frequency of the C-allele is 0.13 for 467 genotyped SBs. Whole genome sequencing of homozygotes for the C-allele and the T-allele in trotter horses is planned. The follow-up research into the EDN3 vicinity is recommended. |
| format | Second cycle, A2E |
| id | RepoSLU13879 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2018 |
| publishDateSort | 2018 |
| record_format | eprints |
| spelling | RepoSLU138792019-10-19T23:15:04Z https://stud.epsilon.slu.se/13879/ Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? Novácková, Linda Animal genetics and breeding Standardbred horses (SBs) are used in harness racing. Their common breeding history with Coldblooded trotters (CBTs) led us to the hypothesis, that the same region associated with race performance in CBTs, may be linked to the performance of SBs as well. EDN3 SNP (g.22:45748491C>T), was found to be associated (p<0.05) with performance traits in CBTs (Fegraeus, 2017). EDN3 SNP has a high frequency of the T-allele in breeds related to races and to the high performance (Fegraeus, 2017). EDN3 SNP is located in the vicinity of endothelin-3 (EDN3) gene. Protein endothelin-3 (EDN3) is responsible for the development of the enteric nervous system and the melanin distribution in the foetus and it is involved in the blood regulation. Custom designed TaqMan SNP Genotyping Assays (Applied Biosystems StepOnePlusTM Instrument by Life Technologies) were used to genotype 550 SBs. Four hundred and fifty-seven genotypes were obtained and no statistically significant association between racing performance traits and genotype was found. Several associations (<0.05) between performance traits and other fixed effects – sex and year of birth - were found. The T-allele frequency of EDN3 SNP is 0.87 and the frequency of the C-allele is 0.13 for 467 genotyped SBs. Whole genome sequencing of homozygotes for the C-allele and the T-allele in trotter horses is planned. The follow-up research into the EDN3 vicinity is recommended. Americký klusák je oblíbené dostihové plemeno. Jeho historie je spojena se Severským chladnokrevným klusákem (CBT), v jehož chovném programu byl Americký klusák využíván pro zlepšení dostihových vlastností. Tato skutečnost nás vedla k hypotéze, že stejná oblast genomu, která je spojena s výkonností CBTs, může být zodpovědná za výkonnosti Amerického klusáka. EDN3 SNP (g.22:45748491C>T) je zodpovědný (p<0.05) za výkonnost Severského chladnokrevného klusáka (Fegraeus, 2017). Frekvence T-alely v EDN3 SNP je vysoká v plemenech využívaných pro dostihový sport a náročnější jezdecký sport. EDN3 SNP se nachází v blízkosti genu endothelin-3 (EDN3). Protein endothelin-3 (EDN3) je zodpovědný za vývoj nervů trávicí soustavy a distribuci melaninu v plodu koně a také se podílí na regulaci krevního tlaku. Zakázkově navržený TaqMan SNP test genotypizace (Aplikované Biosystémy StepOnePlusTM nástrojem Life Technologies) byl použit ke genotypizaci 550 vzorků DNA Amerického klusáckého koně. Čtyři sta šedesát sedm genotypů bylo získáno, a přitom nebyla nalezena statisticky signifikantní asociace mezi žádnou proměnnou reprezentující výkonnost a genotypem. Bylo nalezeno několik asociací (<0.05) mezi výkonnostními proměnnými a fixními efekty – pohlaví a rok narození. Frekvence T-alely v EDN3 SNP je 0,8 a C-alely je 0,13. Sekvenování celého genomu je plánováno na homozygotech pro C a T alelu u vybraných koní klusáckých plemen. Navazující výzkum oblasti genu EDN3 je doporučen. 2018-10-19 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/13879/17/Novackova_L_180901.pdf Novácková, Linda, 2018. Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses?. Second cycle, A2E. Uppsala: (VH) > Dept. of Animal Breeding and Genetics (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-670.html> urn:nbn:se:slu:epsilon-s-9873 eng |
| spellingShingle | Animal genetics and breeding Novácková, Linda Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title | Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title_full | Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title_fullStr | Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title_full_unstemmed | Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title_short | Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses? |
| title_sort | is a potential regulatory region in the vicinity of edn3 linked to the performance in standardbred horses? |
| topic | Animal genetics and breeding |
| url | https://stud.epsilon.slu.se/13879/ https://stud.epsilon.slu.se/13879/ |