Bakterier och svampar påvisade i ögonprover från svenska hästar samt bakteriernas antibiotikakänslighet
Ocular infections are a frequent encountered condition in horses and veterinarians in both field and hospital practice are involved in therapy decisions. Before treatment of a suspected ocular infection, cytology along with culture and susceptibility testing is recommended to guide the choice of app...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco sueco |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/13521/ |
| Sumario: | Ocular infections are a frequent encountered condition in horses and veterinarians in both field and hospital practice are involved in therapy decisions. Before treatment of a suspected ocular infection, cytology along with culture and susceptibility testing is recommended to guide the choice of appropriate topical therapy. However, treatment can be required before results from these tests are available and the choice of treatment strategy is therefore often empiric. Empiric choice of antibiotics relies on the knowledge of the normal and pathogen microbial flora in horse eyes. The prevalence of these bacteria has, however, been shown to differ in multiple aspects, for example in geographic location.
The purpose of this study was to describe the bacterial and fungal isolates recovered from ophthalmologic samples in Swedish horses. The study was retrospective and descriptive, consisting of previous sample results collected from the register of the Swedish National Veterinary Institute (Statens Veterinärmedicinska Anstalt, SVA). The study material consisted of sample results from microbial culture performed at SVA between the years 2012 and 2016. The sample material was collected from horses’ eyes in association with veterinary examination. The microbial results and MIC values are described and interpreted for the isolates that was tested. These MIC values were interpreted with breakpoints to establish if the bacteria was wild type or resistant, the wild type was classified as a treatable bacteria.
A total of 329 horses were included in this study, and as sample-taking was performed in more than one occasion for several horses there were 385 samples overall. A total of 331 aerobic bacterial isolates were recovered. The most frequently encountered aerobic bacterial species were Streptococcus spp. (24 %), Moraxella spp. (7 %) and Staphylococcus spp. (6 %). No specific infection could be detected for 160 samples and no bacterial growth was detected in 59 samples. Anaerobic bacterial culture was performed in 88 samples but there were no anaerobic bacteria detected in these samples. A majority (75 %) of the samples yielded a mixed growth. Fungal culture was performed in 45 samples, either in combination with aerobic bacterial culture (n = 34) or fungal culture alone (n = 11). Yeast was most frequently encountered (n = 14) and only one sample of Aspergillus spp. was detected.
The majority of the susceptibility tested bacterial isolates were found to be mainly of wild type. Streptococcus spp., the most prevalent group of bacterial species recovered, were in general of wild type in tests of all included antibiotics (77-100% wild type). On the other hand Staphylococcus spp. differed as when tested against fusidic acid it showed only 27 % (3/11) were wild type, whereas when testing against penicillin there were 75 % (15/20) wild type and for the rest of the antibiotics 85-100% were wild type.
This study describes microbial isolates from ophthalmologic samples from horses in Sweden, as well as some of their anticipated antibiotic sensitivities. However, there is a need of further studies to evaluate if the microbes recovered were related to ocular disease or merely a reflection of the normal microbial flora. The findings from this study, along with further studies, may provide guidance in the empirical choices of therapy and potentially improve treatment outcomes and as well as reduce inappropriate use of antibiotics. |
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