En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto
This study was performed in order to evaluate if the method of collecting uterine secretion by the tampon-method is representative for use in studies of uterine secretions in mares. Uterine secretion collected by tampon in six mares was compared with secretion collected by aspiration, regarded to be...
| Autor principal: | |
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| Formato: | L3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Obstetrics and Gynaecology
2003
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| Materias: |
| _version_ | 1855572124731703296 |
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| author | Clasen, Henriette |
| author_browse | Clasen, Henriette |
| author_facet | Clasen, Henriette |
| author_sort | Clasen, Henriette |
| collection | Epsilon Archive for Student Projects |
| description | This study was performed in order to evaluate if the method of collecting uterine secretion by the tampon-method is representative for use in studies of uterine secretions in mares. Uterine secretion collected by tampon in six mares was compared with secretion collected by aspiration, regarded to be a native sample, at the Institution of Obstetrics and Gynaecology, SLU, Uppsala. Proteinanalysis was made at the Department of Farmaceutic Bioscience, toxicological unit, Uppsala University. Cytological smears and ultrasonography was used to assess if the tampon had caused an inflammatoric reaction that could influence the protein content of the samples.
The results suggest that the tampon and aspirat method do not differ when it comes to proteinconcentration, but the distribution of proteins differ between these different collection methods. This difference could not likely be explained by means of proteins due to an inflammatory reaction from the tampon, since neither the cytological nor the ultrasonographical examination gave any indications of inflammation. It is therefore suggested that uterine secretions sampled by the tampon method should be interpreted with caution. |
| format | L3 |
| id | RepoSLU12754 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | SLU/Dept. of Obstetrics and Gynaecology |
| publisherStr | SLU/Dept. of Obstetrics and Gynaecology |
| record_format | eprints |
| spelling | RepoSLU127542017-10-19T10:25:13Z En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto Clasen, Henriette sto tampong uterussekret 2DE This study was performed in order to evaluate if the method of collecting uterine secretion by the tampon-method is representative for use in studies of uterine secretions in mares. Uterine secretion collected by tampon in six mares was compared with secretion collected by aspiration, regarded to be a native sample, at the Institution of Obstetrics and Gynaecology, SLU, Uppsala. Proteinanalysis was made at the Department of Farmaceutic Bioscience, toxicological unit, Uppsala University. Cytological smears and ultrasonography was used to assess if the tampon had caused an inflammatoric reaction that could influence the protein content of the samples. The results suggest that the tampon and aspirat method do not differ when it comes to proteinconcentration, but the distribution of proteins differ between these different collection methods. This difference could not likely be explained by means of proteins due to an inflammatory reaction from the tampon, since neither the cytological nor the ultrasonographical examination gave any indications of inflammation. It is therefore suggested that uterine secretions sampled by the tampon method should be interpreted with caution. Denna studie syftade till att undersöka om prover tagna med tampong är representativa för att kunna användas i studier av uterussekret hos sto. Uterussekret samlat med tampong jämfördes med prover samlat via aspiration, vilket anses ge ett fysiologiskt representativt prov, från sex ston vid Institutionen för obstetrik och gynekologi, SLU, Uppsala, och proteinanalyser utfördes vid Institutionen för farmaceutisk biovetenskap, avd. för toxikologi, Uppsala Universitet. Cellutstryk och ultraljud användes för att undersöka om tampongen hade inducerat en inflammatorisk reaktion i uterus som skulle kunna påverka proteininnehållet i proverna. Resultaten indikerar att proteinkoncentrationen inte skiljer sig mellan tampong- och aspiratmetoden, men att proteininnehållet är olika i prover samlade med respektive metod. Denna skillnaden har troligtvis ej orsakad av proteiner från en inflammation inducerad av tampongen, då varken cytologi- eller ultraljudsundersökningar gav indikationer på inflammation. Således bör troligen uterussekret samlat med tampongmetoden tolkas med försiktighet. SLU/Dept. of Obstetrics and Gynaecology 2003 L3 swe eng https://stud.epsilon.slu.se/12754/ |
| spellingShingle | sto tampong uterussekret 2DE Clasen, Henriette En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title | En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title_full | En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title_fullStr | En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title_full_unstemmed | En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title_short | En proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| title_sort | en proteomisk utvärdering av olika metoder för att samla uterussekret hos sto |
| topic | sto tampong uterussekret 2DE |