Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses
General anaesthesia in horses is often followed by postoperative complications, including myopathy. Many colic patients are compromised by preoperative stressors, such as starvation, trailer rides, physical stress and pain, plus postoperative physical stress in connection with standing up after recu...
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| Formato: | L3 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Large Animal Clinical Sciences
2004
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| Materias: |
| _version_ | 1855572121299714048 |
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| author | Cedervind, Holly |
| author_browse | Cedervind, Holly |
| author_facet | Cedervind, Holly |
| author_sort | Cedervind, Holly |
| collection | Epsilon Archive for Student Projects |
| description | General anaesthesia in horses is often followed by postoperative complications, including myopathy. Many colic patients are compromised by preoperative stressors, such as starvation, trailer rides, physical stress and pain, plus postoperative physical stress in connection with standing up after recumbency. These factors may influence muscle glycogen stores, since glycogen is an important substrate source in metabolism. The aim of this study was to investigate muscle glycogen content with histochemical techniques, before and after lengthy anaesthesia in both healthy horses and in colic patients.
Seven colic patients and 5 healthy warmblooded trotters were anaesthetized (duration > 3 hr). Patients did not eat (9-51 hr) prior to surgery and had been transported large distances to surgery (53 -320 km). Healthy horses were denied food (12 h) prior to anaesthesia. Choice of premedication and induction agents was left to the anaesthetist. Anaesthesia was maintained with isoflurane with horses in dorsal recumbency. M. gluteus medius was biopsied in all horses at induction, end of anaesthesia and in some horses, 24 hours after anaesthesia. Samples were cut in a cryostat and stained for myosin ATP-ase activity after pre-incubation at pH 4.6, to identify type I, IIA and IIB fibres, and with Periodic Acid-Schiff's reagent, in order to subjectively evaluate 'low' or 'high' glycogen content in fibres.
Healthy horses and two patients had a high glycogen content in all fibre types at all biopsy times. Three patients had a high glycogen content in most fibres at induction of anaesthesia, but had a few low staining fibres at end of anaesthesia. A low glycogen content in most fibres (primarily type I fibres) was seen at induction of anaesthesia in the other four patients. In three of these horses, type IIB fibres also had a low glycogen content. Changes in glycogen content were still evident at end of anaesthesia and even 24 hours after standing up in recovery. The horses with a poor preoperative physical status had many fibres with low glycogen content.
This study has shown that glycogen availability in muscle fibres may be an important factor to consider in anaesthesia and in the postoperative recovery period, and that this could be related to preoperative stress factors. |
| format | L3 |
| id | RepoSLU12735 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | SLU/Dept. of Large Animal Clinical Sciences |
| publisherStr | SLU/Dept. of Large Animal Clinical Sciences |
| record_format | eprints |
| spelling | RepoSLU127352017-10-19T11:55:59Z Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses Glykogeninnehåll i olika muskelfibertyper innan och efter långvarig narkos hos häst Cedervind, Holly equine anaesthesia colic muscle glycogen histochemistry General anaesthesia in horses is often followed by postoperative complications, including myopathy. Many colic patients are compromised by preoperative stressors, such as starvation, trailer rides, physical stress and pain, plus postoperative physical stress in connection with standing up after recumbency. These factors may influence muscle glycogen stores, since glycogen is an important substrate source in metabolism. The aim of this study was to investigate muscle glycogen content with histochemical techniques, before and after lengthy anaesthesia in both healthy horses and in colic patients. Seven colic patients and 5 healthy warmblooded trotters were anaesthetized (duration > 3 hr). Patients did not eat (9-51 hr) prior to surgery and had been transported large distances to surgery (53 -320 km). Healthy horses were denied food (12 h) prior to anaesthesia. Choice of premedication and induction agents was left to the anaesthetist. Anaesthesia was maintained with isoflurane with horses in dorsal recumbency. M. gluteus medius was biopsied in all horses at induction, end of anaesthesia and in some horses, 24 hours after anaesthesia. Samples were cut in a cryostat and stained for myosin ATP-ase activity after pre-incubation at pH 4.6, to identify type I, IIA and IIB fibres, and with Periodic Acid-Schiff's reagent, in order to subjectively evaluate 'low' or 'high' glycogen content in fibres. Healthy horses and two patients had a high glycogen content in all fibre types at all biopsy times. Three patients had a high glycogen content in most fibres at induction of anaesthesia, but had a few low staining fibres at end of anaesthesia. A low glycogen content in most fibres (primarily type I fibres) was seen at induction of anaesthesia in the other four patients. In three of these horses, type IIB fibres also had a low glycogen content. Changes in glycogen content were still evident at end of anaesthesia and even 24 hours after standing up in recovery. The horses with a poor preoperative physical status had many fibres with low glycogen content. This study has shown that glycogen availability in muscle fibres may be an important factor to consider in anaesthesia and in the postoperative recovery period, and that this could be related to preoperative stress factors. Postoperativa komplikationer efter allmännarkos är vanligare hos häst jämfört med andra djurslag. En ofta förekommande komplikation är postoperativ myopati. Före en operation kan många kolikpatienter ha varit utan foder en längre tid, åkt långt i hästtransport, upplevt fysisk stress och smärta. Postoperativt kan fysisk stress förekomma i samband med att hästarna reser sig efter narkosen. Dessa faktorer skulle kunna påverka glykogenförråden i muskeln, eftersom glykogen är ett viktigt energisubstrat. Syftet med studien var att undersöka glykogeninnehållet i muskulaturens fibrer med histokemiska tekniker, innan och efter långvarig narkos, hos friska hästar och kolikpatienter. Sju kolikpatienter och 5 friska hästar ingick i studien (narkoslängd >3 h). Patienterna hade ej utfodrats (9-51 h) innan operationen och de hade åkt hästtransport (53-320 km). De friska hästarna hade ej utfodrats (12 h) innan sövningen. Anestesin inducerades med guaifenesin/thiopental eller ketamin/diazepam och underhölls med isofluran och syrgas. Hästarna låg i ryggläge och andades spontant eller ventilerades mekaniskt. Intravenösa elektrolyter administrerades och vid hypotension gavs dextran eller dobutamin. Biopsier från M. gluteus medius togs vid induktion och vid slutet av narkos samt hos vissa hästar, 24h efter narkosens slut. Prover snittades i kryostat och färgades dels för myosin ATPas aktivitet efter preinkubering vid pH 4,6, för att identifiera typ I, IIA och IIB fibrer, och dels med Periodic Acid-Schiffs reagens, för att subjektivt utvärdera högt respektive lågt glykogeninnehåll. De friska hästarna hade ett högt glykogeninnehåll i alla fibertyper vid alla biopsitagningstillfällena. Tre av patienterna hade ett högt glykogeninnehåll i de flesta fibrer vid anestesins induktion. Ett lågt glykogeninnehåll i ett flertal fibrer (framförallt typ I fibrer) sågs vid anestesins induktion hos fyra patienter. Hos tre av dessa hästar hade också en del typ IIB fibrer ett lågt glykogeninnehåll. Förändringarna i glykogeninnehåll kvarstod direkt efter narkosens slut samt även 24 timmar efter resning. De hästar som hade sämst preoperativ status var också de som uppvisade ett flertal fibrer med lågt glykogeninnehåll vid alla biopsitagningstillfällen. Den begränsade glykogentillgången i fibrerna hos vissa av kolikpatienterna är troligen relaterat till preoperativa stressfaktorer. Denna studie har visat att glykogentillgången i en del muskelfibrer är begränsad hos vissa kolikpatienter inför narkos, vilket kan vara en faktor av betydelse för narkosens förlopp och den postoperativa återhämtningen. SLU/Dept. of Large Animal Clinical Sciences 2004 L3 eng swe https://stud.epsilon.slu.se/12735/ |
| spellingShingle | equine anaesthesia colic muscle glycogen histochemistry Cedervind, Holly Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title | Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title_full | Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title_fullStr | Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title_full_unstemmed | Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title_short | Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| title_sort | glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses |
| topic | equine anaesthesia colic muscle glycogen histochemistry |