Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration
Alcohol addiction is one of the most common drug addictions amongst societies. Alcohol abuse, can lead serious injuries, severe diseases or even death. Despite intensive research, complete knowledge about alcohol addiction and a specific "ethanol receptor" has not been found yet. However, persistent...
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| Formato: | Otro |
| Lenguaje: | sueco Inglés |
| Publicado: |
2009
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| Acceso en línea: | https://stud.epsilon.slu.se/12697/ |
| _version_ | 1855572114497601536 |
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| author | Trinh, Hung |
| author_browse | Trinh, Hung |
| author_facet | Trinh, Hung |
| author_sort | Trinh, Hung |
| collection | Epsilon Archive for Student Projects |
| description | Alcohol addiction is one of the most common drug addictions amongst societies. Alcohol abuse, can lead serious injuries, severe diseases or even death. Despite intensive research, complete knowledge about alcohol addiction and a specific "ethanol receptor" has not been found yet. However, persistent researchers discovered that ethanol consumption and abuse is associated to the brain reward pathway and acts on the receptors of the system to elicit addiction. This project aimed to identify and characterize genes involved in the vulnerability for developing alcohol addiction. mRNA expression levels of neurotransmitter receptors were correlated to ethanol self administration in 11 rat brains. Measurement of the neurotransmitter receptor's mRNA expression was carried out by using quantitative real-time PCR. The tested brain areas were the ventral tegmental area (VTA), nucleus accumbens ( NAc), substantial nigra (Sn) and the caudate putamen (CPu). These areas have important roles in the reward pathway and contain the mesocorticolimbic dopamine pathway, which is believed to be behind the reward mechanisms. We found that the iontropic glutamate AMPA receptor subtype 3 (GluR3) in VTA correlated with ethanol self-administration. Two additional correlations were found in the NAc; the glutamate AMPA receptor subtype 3 (GluR3) and the dopamine receptor subtype 2 (DR2). Glutamatergic and dopaminergic receptors in VTA and NAc have been pointed out to be important in alcohol abuse, addiction behavior and reward.
The correlated results showed a collaboration between the glutamatergic and the dopaminergic system in the VTA and NAc, which actuate alcohol addiction and behavior. |
| format | Otro |
| id | RepoSLU12697 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| record_format | eprints |
| spelling | RepoSLU126972017-10-20T11:16:10Z https://stud.epsilon.slu.se/12697/ Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration Trinh, Hung Dept. of Plant Biology and Forest Genetics (until 131231) Human medicine, health, and safety Alcohol addiction is one of the most common drug addictions amongst societies. Alcohol abuse, can lead serious injuries, severe diseases or even death. Despite intensive research, complete knowledge about alcohol addiction and a specific "ethanol receptor" has not been found yet. However, persistent researchers discovered that ethanol consumption and abuse is associated to the brain reward pathway and acts on the receptors of the system to elicit addiction. This project aimed to identify and characterize genes involved in the vulnerability for developing alcohol addiction. mRNA expression levels of neurotransmitter receptors were correlated to ethanol self administration in 11 rat brains. Measurement of the neurotransmitter receptor's mRNA expression was carried out by using quantitative real-time PCR. The tested brain areas were the ventral tegmental area (VTA), nucleus accumbens ( NAc), substantial nigra (Sn) and the caudate putamen (CPu). These areas have important roles in the reward pathway and contain the mesocorticolimbic dopamine pathway, which is believed to be behind the reward mechanisms. We found that the iontropic glutamate AMPA receptor subtype 3 (GluR3) in VTA correlated with ethanol self-administration. Two additional correlations were found in the NAc; the glutamate AMPA receptor subtype 3 (GluR3) and the dopamine receptor subtype 2 (DR2). Glutamatergic and dopaminergic receptors in VTA and NAc have been pointed out to be important in alcohol abuse, addiction behavior and reward. The correlated results showed a collaboration between the glutamatergic and the dopaminergic system in the VTA and NAc, which actuate alcohol addiction and behavior. Alkoholberoende är ett av de vanligaste drogberoendena hos olika samhällen. Alkoholmissbruk kan leda till skador, allvarliga sjukdomar och till och med dödlig utgång. Trots intensiv forskning, är kunskapen om alkoholmissbruk ofullständig och en särskild "etanol receptor" har ännu inte hittas. Men ihärdiga forskare har funnit att etanol konsumtion och missbruk är kopplade till hjärnans belöningssystem och verkar på systemets receptorer för att framkalla beroende. Med detta projekt försökte vi att identifiera och karaktärisera gener som är involverade i benägenheten för att utveckla alkoholism. Genuttrycket av de neurotransmittoriska receptorerna korrelerades med etanol självadministrering hos 11 råttor. Uttrycket av receptorerna mättes kvantitativt med realtids-PCR. De testades hjärnområden var det ventrala tegmentområdet (VTA), accumbenskärnan (NAc), substantia nigra (Sn) och caudate putamen (CPu). Dessa områden har viktiga roller i belöningssystemet och där ligger även den mesokortikolimbiska dopamin systemet som anses vara själva belöningsmekanismen. Vi fann att nivå av den ionotrop glutamat AMPA receptor subtyp 3 (GluR3) i VTA korrelerade till etanol självadministrering. I NAc fann vi även korrelation med GluR3 och dopamin receptor subtyp 2 (DR2). Glutamat och dopamin receptorer i VTA och NAc är utpekade som viktiga i alkoholmissbruk, beroende och i belöningssystemet. De korrelerande resultaten indikerar en samverkan mellan glutamat och dopamin systemet, som i sin tur påverkar alkoholberoendet och beteende. 2009-03-20 Other NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/12697/1/trinh_h_171020.pdf Trinh, Hung, 2009. Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration. UNSPECIFIED, Uppsala. Uppsala: (NL, NJ) > Dept. Of Plant Biology <https://stud.epsilon.slu.se/view/divisions/LMNR=3A480.html> urn:nbn:se:slu:epsilon-s-8083 eng |
| spellingShingle | Dept. of Plant Biology and Forest Genetics (until 131231) Human medicine, health, and safety Trinh, Hung Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title | Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title_full | Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title_fullStr | Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title_full_unstemmed | Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title_short | Identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| title_sort | identification and characterization of genes that are involved in reward mechanisms in ethanol self-administration |
| topic | Dept. of Plant Biology and Forest Genetics (until 131231) Human medicine, health, and safety |
| url | https://stud.epsilon.slu.se/12697/ https://stud.epsilon.slu.se/12697/ |