Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam
The aim of this study was to get an overview of prevalence and resistance patterns of Salmonella enterica in chicken as well as antibiotic use and knowledge of antibiotic resistance among farmers in the Can Tho region, Vietnam. Material was sampled on local farms and markets during six weeks in Octo...
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| Formato: | Otro |
| Lenguaje: | sueco Inglés |
| Publicado: |
2009
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| Acceso en línea: | https://stud.epsilon.slu.se/12340/ |
| _version_ | 1855572045398540288 |
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| author | Nilsson, Oskar |
| author_browse | Nilsson, Oskar |
| author_facet | Nilsson, Oskar |
| author_sort | Nilsson, Oskar |
| collection | Epsilon Archive for Student Projects |
| description | The aim of this study was to get an overview of prevalence and resistance patterns of Salmonella enterica in chicken as well as antibiotic use and knowledge of antibiotic resistance among farmers in the Can Tho region, Vietnam. Material was sampled on local farms and markets during six weeks in October to November of 2008. Twelve markets were selected and each market was sampled once for a total of 96 samples of neck skin of chicken. In addition 20 farms were selected and rectal swabs were collected on a total of 96 chickens. Analyses of market samples were based on the principles of the Nordic Committee on Food Analysis but adapted to local practises. The sampling from farms was based on procedures used in humans and studies of other bacteria in chicken, thus being more uncertain in its outcome. A total of 11 samples, nine from farms and two from markets, were positive or uncertain on polyvalent antiserum testing and hence sent to Sweden for final confirmation and serotyping. Surprisingly only one out of the eleven isolates was shown to belong to the Salmonella enterica species, namely S. Enteritidis. The reason to the large number of negative samples has to be contributed to the inexperience of myself as well as to the use of modified methods in a foreign laboratory environment. The positive sample was tested for antibiotic resistance showing resistance to four different antibiotics; ampicillin, streptomycin, sulfamethoxazol and tetracyclin. Farmer awareness was studied through a questionnaire and showed low knowledge of antibiotic resistance development and moreover that antibiotics are used as a prophylactic and dependent on availability rather than in regard to its effect on specific disease causing agents. |
| format | Otro |
| id | RepoSLU12340 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| record_format | eprints |
| spelling | RepoSLU123402017-10-18T11:43:09Z https://stud.epsilon.slu.se/12340/ Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam Nilsson, Oskar Animal diseases Food contamination and toxicology The aim of this study was to get an overview of prevalence and resistance patterns of Salmonella enterica in chicken as well as antibiotic use and knowledge of antibiotic resistance among farmers in the Can Tho region, Vietnam. Material was sampled on local farms and markets during six weeks in October to November of 2008. Twelve markets were selected and each market was sampled once for a total of 96 samples of neck skin of chicken. In addition 20 farms were selected and rectal swabs were collected on a total of 96 chickens. Analyses of market samples were based on the principles of the Nordic Committee on Food Analysis but adapted to local practises. The sampling from farms was based on procedures used in humans and studies of other bacteria in chicken, thus being more uncertain in its outcome. A total of 11 samples, nine from farms and two from markets, were positive or uncertain on polyvalent antiserum testing and hence sent to Sweden for final confirmation and serotyping. Surprisingly only one out of the eleven isolates was shown to belong to the Salmonella enterica species, namely S. Enteritidis. The reason to the large number of negative samples has to be contributed to the inexperience of myself as well as to the use of modified methods in a foreign laboratory environment. The positive sample was tested for antibiotic resistance showing resistance to four different antibiotics; ampicillin, streptomycin, sulfamethoxazol and tetracyclin. Farmer awareness was studied through a questionnaire and showed low knowledge of antibiotic resistance development and moreover that antibiotics are used as a prophylactic and dependent on availability rather than in regard to its effect on specific disease causing agents. Målet med studien var att undersöka prevalens och antibiotikaresistensmönster för Salmonella enterica i kyckling i Can Tho regionen i södra Vietnam. Utöver detta syftade studien till att undersöka kunskap och medvetenhet hos fjäderfäproducenterna i regionen avseende antibiotikaanvändning och resistensutveckling. Provtagningen utfördes på lokala gårdar och marknader under sex veckor i oktober och november 2008. Tolv marknader valdes ut och varje marknad provtogs en gång. Sammanlagt togs 96 prover av nackskinn från kyckling. Utöver detta valdes 20 gårdar ut, från vilka totalt 96 kloakprover från levande kyckling erhölls. Marknadsprover analyserades enligt NMKL-metoden, men modifierades efter lokala förutsättningar. Gårdsproverna följde ingen etablerad metod utan utgick från hur analysmetoder på prover från människa går till samt erfarenheter från SVA på liknande provtagning vid undersökning av andra bakterier. Således var resultatet av den senare analysen mer osäkert. Totalt 11 prover, nio gårdsprover och två marknadsprover, var positiva eller tveksamma vid agglutination med polyvalent antiserum och skickades till Sverige för slutgiltig konfirmering och serotypning. Förvånande nog tillhörde endast ett av elva skickade bakterieisolat Salmonella enterica, serotypat som S. Enteritidis. Orsaken till det stora antalet osäkra och vidare negativa proverna måste härledas till personlig brist på erfarenhet av bakteriologiska studier samt användandet av modifierade och relativt oprövade metoder i en främmande laboratoriemiljö. Det positiva provet testades för antibiotikaresistens och visade sig vara en multiresistent stam med höga MIC-värden mot följande antibiotika; ampicillin, streptomycin, sulfamethoxazol och tetracyklin. Producentmedvetenhet undersöktes med ett skriftligt frågeformulär och visade på en mycket liten kunskap om antibiotikaresistensutveckling samt att antibiotika används profylaktiskt och efter tillgång och kostnad snarare än med avseende på aktuell sjukdomsagens och passande antibiotikabehandling. 2009-09-04 Other NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/12340/1/nilsson_o_171018.pdf Nilsson, Oskar, 2009. Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam. UNSPECIFIED, Uppsala. Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-7995 eng |
| spellingShingle | Animal diseases Food contamination and toxicology Nilsson, Oskar Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title | Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title_full | Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title_fullStr | Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title_full_unstemmed | Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title_short | Prevalence and antibiotic resistance of Salmonella enterica in poultry and raw meat in the Can Tho province, Vietnam |
| title_sort | prevalence and antibiotic resistance of salmonella enterica in poultry and raw meat in the can tho province, vietnam |
| topic | Animal diseases Food contamination and toxicology |
| url | https://stud.epsilon.slu.se/12340/ https://stud.epsilon.slu.se/12340/ |