Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik
The concentration of acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two acute phase proteins that show the largest inc...
| Autor principal: | |
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| Formato: | Otro |
| Lenguaje: | sueco sueco |
| Publicado: |
2005
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| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/11861/ |
| _version_ | 1855571954864488448 |
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| author | Borg, Niklas |
| author_browse | Borg, Niklas |
| author_facet | Borg, Niklas |
| author_sort | Borg, Niklas |
| collection | Epsilon Archive for Student Projects |
| description | The concentration of acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two acute phase proteins that show the largest increase in serum concentration as a result of inflammatory processes. The haptoglobin concentration has been shown to correlate to clinical signs as well as inflammatory processes found at post mortem examination. This feature could be useful prognosis of the individual as well as for monitoring the health status of a herd or identifying carcasses that shold be examined closer at the slaughter-house. In this paper, a method for analysis of haptoglobin in milk has been modified for use with bovine sera. The method which uses optical biosensor technology is based on the haemoglobin binding properties of haptoglobin. It utilizes the interaction between analyte (haemoglobin) and biological ligand (haptogobin) which results in an optically detectable alteration in mass on the sensor surface. |
| format | Otro |
| id | RepoSLU11861 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish swe |
| publishDate | 2005 |
| publishDateSort | 2005 |
| record_format | eprints |
| spelling | RepoSLU118612017-10-13T09:26:10Z https://stud.epsilon.slu.se/11861/ Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik Borg, Niklas Veterinary science and hygiene - General aspects The concentration of acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two acute phase proteins that show the largest increase in serum concentration as a result of inflammatory processes. The haptoglobin concentration has been shown to correlate to clinical signs as well as inflammatory processes found at post mortem examination. This feature could be useful prognosis of the individual as well as for monitoring the health status of a herd or identifying carcasses that shold be examined closer at the slaughter-house. In this paper, a method for analysis of haptoglobin in milk has been modified for use with bovine sera. The method which uses optical biosensor technology is based on the haemoglobin binding properties of haptoglobin. It utilizes the interaction between analyte (haemoglobin) and biological ligand (haptogobin) which results in an optically detectable alteration in mass on the sensor surface. Serumkoncentrationen av akutfasproteiner förändras som svar på inflammation, infektion eller trauma, vilket gör dem till potentiella biomarkörer för ospecifika sjukdomstillstånd. Hos nötkreatur är haptoglobin och serumamyloid A de akutfasproteiner som visar kraftigast ökning vid inflammatoriska tillstånd. Haptoglobinkoncentrationen har också visats korrelera med kliniska symptom och inflammatoriska förändringar funna vid obduktion. Detta skulle kunna användas för att ställa prognos på individnivå men även för övervakning av hälsoläge i besättningar samt på slakteri för selektion av slaktkroppar som bör undersökas noggrannare. I detta arbete beskrivs hur en metod för analys av haptoglobin i mjölk anpassats för analys av haptoglobin i bovint serum. Metoden utnyttjar haptoglobinets hemoglobinbindande egenskaper och är utvecklad med hjälp av optisk biosensorteknik. Grunden för analysen är en interaktion mellan analyt (hemoglobin) och biologisk ligand (haptoglobin) vilken resulterar i en optiskt detekterbar förändrad massa på sensorytan. 2005-12-22 Other NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/11861/1/borg_n_171013.pdf Borg, Niklas, 2006. Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik. UNSPECIFIED, Uppsala. Uppsala: (VH) > Dept. of Clinical Sciences (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-715.html> urn:nbn:se:slu:epsilon-s-7812 swe |
| spellingShingle | Veterinary science and hygiene - General aspects Borg, Niklas Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title | Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title_full | Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title_fullStr | Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title_full_unstemmed | Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title_short | Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| title_sort | analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik |
| topic | Veterinary science and hygiene - General aspects |
| url | https://stud.epsilon.slu.se/11861/ https://stud.epsilon.slu.se/11861/ |