Reagerar grisar immunologiskt mot foderproteiner?
The aim of this study was to detect antibodies in swine serum against proteins in soy beans, wheat, oat and barley. Two methods were used: agar gel immunodiffusion test (AGID), and SDS-PAGE followed by immunoblotting. The sera examined originated (I) from piglets younger than one week (negative cont...
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| Formato: | Otro |
| Lenguaje: | sueco sueco |
| Publicado: |
2008
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| Acceso en línea: | https://stud.epsilon.slu.se/11743/ |
| _version_ | 1855571929849659392 |
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| author | Haggård, Lisa |
| author_browse | Haggård, Lisa |
| author_facet | Haggård, Lisa |
| author_sort | Haggård, Lisa |
| collection | Epsilon Archive for Student Projects |
| description | The aim of this study was to detect antibodies in swine serum against proteins in soy beans, wheat, oat and barley. Two methods were used: agar gel immunodiffusion test (AGID), and SDS-PAGE followed by immunoblotting. The sera examined originated (I) from piglets younger than one week (negative control), (II) pigs from a feed trial with soy bean in a herd with PMWS, and (III) pigs inoculated with Brachyspira hyodysenteriae and Escherichia coli after being fed with soy. The AGID-test was negative concerning all examined sera which were analysed against proteins in soy bean, wheat, oat and barley. In the experiment based on immunoblot antibodies were demonstrated within all three groups of examined pigs. The sizes of the protein fragments demonstrated were 20, 28, 45, 46, 47, 48 and 50 kDa. The pigs from the feed trial were divided into two groups. One group was fed conventional soy and the other group processed soy where the contents of antigenic substances had been minimized. The group of pigs fed with conventional soy formed twice as many bands in the blot as the group fed soy which had been processed. Two out of three piglets showed antibodies against soy which indicates that maternal antibodies against soy can be transferred by the colostrum. |
| format | Otro |
| id | RepoSLU11743 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish swe |
| publishDate | 2008 |
| publishDateSort | 2008 |
| record_format | eprints |
| spelling | RepoSLU117432017-11-16T11:48:16Z https://stud.epsilon.slu.se/11743/ Reagerar grisar immunologiskt mot foderproteiner? Haggård, Lisa Animal feeding The aim of this study was to detect antibodies in swine serum against proteins in soy beans, wheat, oat and barley. Two methods were used: agar gel immunodiffusion test (AGID), and SDS-PAGE followed by immunoblotting. The sera examined originated (I) from piglets younger than one week (negative control), (II) pigs from a feed trial with soy bean in a herd with PMWS, and (III) pigs inoculated with Brachyspira hyodysenteriae and Escherichia coli after being fed with soy. The AGID-test was negative concerning all examined sera which were analysed against proteins in soy bean, wheat, oat and barley. In the experiment based on immunoblot antibodies were demonstrated within all three groups of examined pigs. The sizes of the protein fragments demonstrated were 20, 28, 45, 46, 47, 48 and 50 kDa. The pigs from the feed trial were divided into two groups. One group was fed conventional soy and the other group processed soy where the contents of antigenic substances had been minimized. The group of pigs fed with conventional soy formed twice as many bands in the blot as the group fed soy which had been processed. Two out of three piglets showed antibodies against soy which indicates that maternal antibodies against soy can be transferred by the colostrum. Försöket avsåg att påvisa antikroppar hos grisar mot proteiner i soja, vete, havre och korn. Två metoder användes, dels en agar gel immunodiffuson test (AGID), och dels SDS-PAGE följt av immunoblott (western blot). Sera som undersöktes var tagna från grisar yngre än en vecka (kontrollgrisar), grisar från ett foderförsök med soja i en PMWS-besättning, och grisar som inokulerats med Brachyspira hyodysenteriae och Escherichia coli efter utfodring med soja. AGID-testen gav negativt resultat för alla undersökta serumprover analyserade för att påvisa antikroppar mot proteiner i soja, vete, havre och korn. Vid försök baserat på immunoblott undersöktes immunologiska reaktioner mot soja och här påvisades antikroppar hos alla undersökta grisgrupper. Proteinbanden som grisarna reagerade mot hade storlekarna 20, 28, 45, 46, 47, 48 och 50 kDa. Grisarna från foderförsöket var uppdelade i två grupper. Den ena gruppen utfodrades med konventionell soja och den andra gruppen fick processad soja där innehållet av antigena substanser minimerats. Gruppen som fått den konventionella sojan bildade dubbelt så många band i immunoblotten som den andra gruppen där sojan processats. Två av tre kontrollgrisar hade antikroppar mot soja och detta indikerar att maternala antikroppar mot soja överförs via råmjölken. 2008-01-14 Other NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/11743/1/haggard_l_171116.pdf Haggård, Lisa, 2008. Reagerar grisar immunologiskt mot foderproteiner?. UNSPECIFIED, Uppsala. Uppsala: (VH) > Dept. of Clinical Sciences (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-715.html> urn:nbn:se:slu:epsilon-s-8885 swe |
| spellingShingle | Animal feeding Haggård, Lisa Reagerar grisar immunologiskt mot foderproteiner? |
| title | Reagerar grisar immunologiskt mot foderproteiner? |
| title_full | Reagerar grisar immunologiskt mot foderproteiner? |
| title_fullStr | Reagerar grisar immunologiskt mot foderproteiner? |
| title_full_unstemmed | Reagerar grisar immunologiskt mot foderproteiner? |
| title_short | Reagerar grisar immunologiskt mot foderproteiner? |
| title_sort | reagerar grisar immunologiskt mot foderproteiner? |
| topic | Animal feeding |
| url | https://stud.epsilon.slu.se/11743/ https://stud.epsilon.slu.se/11743/ |