Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp.
The aim of this study was to isolate and identify Lactobacillus species indigenous to the gastrointestinal microflora of Swedish horses. These lactobacilli are to be used as probiotic candidates in research concerning antibiotic-associated diarrhoea in horses. Eight horses, age 6-10 years, were sel...
| Autor principal: | |
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| Formato: | L3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2006
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| Materias: |
| _version_ | 1855571907398598656 |
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| author | Bohlin, Maria |
| author_browse | Bohlin, Maria |
| author_facet | Bohlin, Maria |
| author_sort | Bohlin, Maria |
| collection | Epsilon Archive for Student Projects |
| description | The aim of this study was to isolate and identify Lactobacillus species indigenous to the gastrointestinal microflora of Swedish horses. These lactobacilli are to be used as probiotic candidates in research concerning antibiotic-associated diarrhoea in horses.
Eight horses, age 6-10 years, were selected from a riding school for collection of faeces samples. Man Rogosa Sharp medium and gram staining were used for the selection of supposed lactobacilli. We could identify 33 isolates of supposed lactobacilli. With the use of API tests 22 of these 33 isolates could be identified. Out of the remaining unidentified 11 isolates, five isolates were selected for identification by PCR and by sequencing of the 16S rRNA gene.
In this study we could identify L. acidophilus, L. plantarum, L. fermentum, L. equi, L. mucosae and a previously unknown Lactobacillus sp. in the indigenous gastrointestinal microflora of Swedish horses. We also could identify the lactic acid bacterium (LAB) Leuconostoc lactis. |
| format | L3 |
| id | RepoSLU11621 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2006 |
| publishDateSort | 2006 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU116212017-10-02T10:37:17Z Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. Bohlin, Maria Lactobacillus spp. häst PCR identifiering sekvensanalys The aim of this study was to isolate and identify Lactobacillus species indigenous to the gastrointestinal microflora of Swedish horses. These lactobacilli are to be used as probiotic candidates in research concerning antibiotic-associated diarrhoea in horses. Eight horses, age 6-10 years, were selected from a riding school for collection of faeces samples. Man Rogosa Sharp medium and gram staining were used for the selection of supposed lactobacilli. We could identify 33 isolates of supposed lactobacilli. With the use of API tests 22 of these 33 isolates could be identified. Out of the remaining unidentified 11 isolates, five isolates were selected for identification by PCR and by sequencing of the 16S rRNA gene. In this study we could identify L. acidophilus, L. plantarum, L. fermentum, L. equi, L. mucosae and a previously unknown Lactobacillus sp. in the indigenous gastrointestinal microflora of Swedish horses. We also could identify the lactic acid bacterium (LAB) Leuconostoc lactis. Syftet med detta examensarbete var att isolera och identifiera naturligt förekommande Lactobacillus spp. från faeces hos svenska hästar. Dessa laktobaciller skall sedan användas som probiotiska kandidater vid forskning kring antibiotikaassocierad diarré hos häst. Åtta stycken ridskolehästar i åldern 6-10 år valdes ut för faecesprovtagning. Vid urval av presumtiva Lactobacillus spp. användes utodling på selektivt medium (Man Rogosa Sharp agar), samt gramfärgning. Totalt kunde 33 stycken isolat av presumtiva Lactobacillus spp. identifieras. Av dessa 33 isolat kunde 22 stycken identifieras med API-test. Av de kvarvarande 11 stycken oidentifierade isolaten, valdes 5 stycken isolat ut för identifiering med PCR och sekvensering av 16S rRNA genen. Vid detta försök kunde L. acidophilus, L. plantarum , L. fermentum, L. equi, L. mucosae och en Lactobacillus sp, som inte har beskrivits i litteraturen, identifieras från normala tarmfloran hos friska svenska hästar. Dessutom kunde Leuconostoc lactis som också tillhör gruppen mjölksyrabakterier identifieras. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2006 L3 swe eng https://stud.epsilon.slu.se/11621/ |
| spellingShingle | Lactobacillus spp. häst PCR identifiering sekvensanalys Bohlin, Maria Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title | Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title_full | Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title_fullStr | Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title_full_unstemmed | Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title_short | Undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till Lactobacillus spp. |
| title_sort | undersökning av potentiella probiotiska organismer i tarmmikroflora hos häst med speciell hänsyn till lactobacillus spp. |
| topic | Lactobacillus spp. häst PCR identifiering sekvensanalys |